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Long noncoding RNA CCAT2 correlates with smoking in esophageal squamous cell carcinoma

  • Research Article
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Tumor Biology

Abstract

Esophageal cancer is one of the leading causes of cancer-related mortality, and most esophageal squamous cell carcinoma (ESCC) cases are located in Asian area. Recent studies about long noncoding RNAs (lncRNA) have offered a new perspective for cancer research and provided new approaches to understand the complex regulation network in cancer. Our group has reported that the novel lncRNA colon cancer-associated transcript 2 (CCAT2) has important biological function and could be a potential biomarkers in lung cancer. Here, we performed in silico analysis and characterized the expression profile of CCAT2 in a cohort of esophageal squamous cell carcinoma (ESCC) patients and cell lines. In silico analysis showed that no CpG island is found in the chromosome region of CCAT2, indicating that the expression of CCAT2 is possibly not regulated by DNA methylation. Compared with paired adjacent normal esophageal tissues, CCAT2 was significantly overexpressed in ESCC tissues with an average fold of 7.18. In ESCC cell lines, CCAT2 was mostly upregulated in KYSE410 cell (24.7-fold upregulation) when normalized to normal esophageal epithelium cell line (HEEC) and most CCAT2 transcripts were located in nucleus (>95 %). Statistical analysis showed that CCAT2 expression level was significantly associated with smoking status (P = 0.036). Receiver operative curve and the area under curve were calculated to assess the diagnostic potential of CCAT2. Measured by area under curve (AUC), CCAT2 showed higher diagnostic performance than conventional serum biomarkers, like AFP, CA153, and NSE. In this study, we firstly characterized the expression profile of CCAT2 in ESCC and evaluated its potential diagnostic value as a biomarker.

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Acknowledgments

We thank Professor Xinchen Sun (Department of Radiology, the First Affiliated Hospital of Nanjing Medical University) for gifting ESCC cells. This study is funded by the Natural Science Foundation of China (81372321 to Lin Xu; 81201830 and 81472200 to Rong Yin), Natural Science Foundation for High Education of Jiangsu Province (13KJB320010 to Rong Yin), and Jiangsu Provincial Special Program of Medical Science (BL2012030 to Lin Xu).

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Authors and Affiliations

  1. Department of Thoracic Surgery, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Baiziting 42, Nanjing, 210009, China

    Jie Wang, Mantang Qiu, Youtao Xu, Ming Li, Gaochao Dong, Qixing Mao, Rong Yin & Lin Xu

  2. Department of Scientific Research, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, 210009, China

    Jie Wang & Gaochao Dong

  3. The Fourth Clinical College of Nanjing Medical University, Nanjing, 210000, China

    Mantang Qiu & Qixing Mao

  4. The First Clinical College of Nanjing Medical University, Nanjing, 210000, China

    Youtao Xu

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  1. Jie Wang
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  2. Mantang Qiu
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  3. Youtao Xu
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  4. Ming Li
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  6. Qixing Mao
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  7. Rong Yin
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  8. Lin Xu
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Corresponding authors

Correspondence to Rong Yin or Lin Xu.

Additional information

Jie Wang, Mantang Qiu, and Youtao Xu contributed equally to this work.

Electronic supplementary material

The association of rs6983267 polymorphism of CCAT2 and diseases: searching results from the LincSNP database. (XLS 11 kb)

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Wang, J., Qiu, M., Xu, Y. et al. Long noncoding RNA CCAT2 correlates with smoking in esophageal squamous cell carcinoma. Tumor Biol. 36, 5523–5528 (2015). https://doi.org/10.1007/s13277-015-3220-x

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  • DOI: https://doi.org/10.1007/s13277-015-3220-x

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