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Overexpression of long non-coding RNA TUG1 predicts poor prognosis and promotes cancer cell proliferation and migration in high-grade muscle-invasive bladder cancer

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Tumor Biology

Abstract

Long non-coding RNA TUG1 is involved in the development and progression of a variety of tumors. Little is known about TUG1 function in high-grade muscle-invasive bladder cancer (MIBC). The aims of our study were to determine expression levels of long non-coding RNA TUG1 in tumor tissue, to evaluate its relationship with clinico-pathological features of high-grade MIBC, and to describe its function in MIBC cells in vitro. TUG1 expression levels were determined in paired tumor and adjacent non-tumor bladder tissues of 47 patients with high-grade MIBC using real-time PCR. Cell line T-24 and siRNA silencing were used to study the TUG1 function in vitro. We observed significantly increased levels of TUG1 in tumor tissue in comparison to adjacent non-tumor bladder tissue (P < 0.0001). TUG1 levels were significantly increased in metastatic tumors (P = 0.0147) and were associated with shorter overall survival of MIBC patients (P = 0.0241). TUG1 silencing in vitro led to 34 % decrease in cancer cell proliferation (P = 0.0004) and 23 % reduction in migration capacity of cancer cells (P < 0.0001). We did not observe any significant effects of TUG1 silencing on cell cycle distribution and number of apoptotic cells. Our study confirmed overexpression of TUG1 in MIBC tumor tissue and described its association with worse overall survival in high-grade MIBC patients. Together with in vitro observations, these data suggest an oncogenic role of TUG1 and its potential usage as biomarker or therapeutic target in MIBC.

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Acknowledgments

This work has been financially supported by the Czech Ministry of Health, grant no. 31071A, project MZ CR-RVO (MOU, 00209805) and the Ministry of Education, Youth and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601). We would like to thank Jon B. Smith for proofreading the manuscript.

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Authors and Affiliations

  1. Central European Institute of Technology (CEITEC), Masaryk University, University Campus Bohunice, Building A35, Room 217, Kamenice 5, 625 00, Brno, Czech Republic

    Robert Iliev, Renata Kleinova, Jaroslav Juracek, Zuzana Ozanova, Marek Svoboda & Ondrej Slaby

  2. Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic

    Jaroslav Juracek, Marek Svoboda & Ondrej Slaby

  3. Department of Urologic Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53, Brno, Czech Republic

    Jan Dolezel & Michal Stanik

  4. Department of Urology, University Hospital Brno and Masaryk University Brno, Jihlavska 20, 62500, Brno, Czech Republic

    Michal Fedorko & Dalibor Pacik

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  1. Robert Iliev
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  2. Renata Kleinova
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  3. Jaroslav Juracek
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  4. Jan Dolezel
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  5. Zuzana Ozanova
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  6. Michal Fedorko
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  7. Dalibor Pacik
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  8. Marek Svoboda
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  9. Michal Stanik
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  10. Ondrej Slaby
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Correspondence to Michal Stanik or Ondrej Slaby.

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Iliev, R., Kleinova, R., Juracek, J. et al. Overexpression of long non-coding RNA TUG1 predicts poor prognosis and promotes cancer cell proliferation and migration in high-grade muscle-invasive bladder cancer. Tumor Biol. 37, 13385–13390 (2016). https://doi.org/10.1007/s13277-016-5177-9

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  • DOI: https://doi.org/10.1007/s13277-016-5177-9

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