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Infectivity of cloned begomoviral DNAs: an appraisal

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Abstract

Infectivity of cloned begomoviral DNAs is an important criterion to establish the etiology of the disease it causes, to study viral gene functions and host-virus interactions. Three main methods have been employed to study infectivity; mechanical inoculation with cloned viral DNA using abrasives, Agrobacterium-mediated inoculation (agroinoculation) of cloned viral DNA and bombardment using microprojectiles coated with cloned viral DNA (biolistics). Each method has its own advantages and disadvantages and the adoption of one over the other for demonstrating infectivity depends on various factors. This review compares the various features associated with the above three methods.

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Acknowledgements

AKK acknowledges the research fellowship received from grant no. BCIL/NER-BPMC/2012/1549 from Department of Biotechnology, Government of India (Granted to ID). Research in the ID lab was supported from R&D Grant from University of Delhi and DU-DST PURSE. There are no conflicts of interest associated with this manuscript for any author.

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  1. Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Akhilesh Kumar Kushawaha & Indranil Dasgupta

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Correspondence to Indranil Dasgupta.

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Kushawaha, A.K., Dasgupta, I. Infectivity of cloned begomoviral DNAs: an appraisal. VirusDis. 30, 13–21 (2019). https://doi.org/10.1007/s13337-018-0453-5

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