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Molecular identification of clinical Candida isolates by simple and randomly amplified polymorphic DNA-PCR

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Abstract

Thirty yeast isolates were obtained from specimens taken from different patients (sputum, vaginal and oral swabs). The isolates were identified by phenotyping and molecular methods. Six of the isolates were Candida albicans, identified by germ tube and CHROMagar. Three isolates were identified by CHROMagar as C. krusei. The remaining (21) clinical yeast isolates included C. sphaerica, C. guilliermondii C. kefyr, C. famata, C. glabrata, C. parapsilosis and C. norvegensis identified by Vitek2 and further confirmed by PCR. RAPD-PCR was also used in an attempt to identify DNA “fingerprints” for specific Candida spp. The DNA fingerprints of all Candida spp. except for C. sphaerica were determined.

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Authors and Affiliations

  1. Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq

    Afnan R. A. Al-Tekreeti & Abdulkareem Jasim Hashim

  2. Department of Medical and Molecular Biotechnology, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq

    Mohammad M. F. Al-Halbosiy & Farooq Ibrahem Mohammad

  3. Department of Biology, College of Education, Iraqia University, Baghdad, Iraq

    Batol Imran Dheeb

  4. Department of Biotechnology, College of Science, University of Diyala, Diyala, Iraq

    Anaam Fuad Hussain Al-Zuhairi

Authors
  1. Afnan R. A. Al-Tekreeti
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  2. Mohammad M. F. Al-Halbosiy
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  3. Batol Imran Dheeb
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  4. Abdulkareem Jasim Hashim
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  5. Anaam Fuad Hussain Al-Zuhairi
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  6. Farooq Ibrahem Mohammad
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Correspondence to Mohammad M. F. Al-Halbosiy.

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Al-Tekreeti, A.R.A., Al-Halbosiy, M.M.F., Dheeb, B.I. et al. Molecular identification of clinical Candida isolates by simple and randomly amplified polymorphic DNA-PCR. Arab J Sci Eng 43, 163–170 (2018). https://doi.org/10.1007/s13369-017-2762-1

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  • DOI: https://doi.org/10.1007/s13369-017-2762-1

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