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Micropropagation of Dendrocalamus asper {Schult. & Schult. F.} Backer ex k. Heyne): an exotic edible bamboo

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Abstract

Effect of season, media type, carbon source, growth regulators and transplanting media on micropropagation of Dendrocalamus asper, an important bamboo species, was examined. The season of explant collection played an important role in axillary bud sprouting and spring (February–April) was found to be the best period for explant collection. Among the different media MS was found to be the best for micropropagation. Maximum numbers (4.83/explant) of shoots were initiated in MS + 15 μM BAP. For shoot multiplication, MS medium supplemented with 10 μM BAP and 75 μM Adenine sulfate was used. BAP was superior to KIN for both explant establishment, as well as, shoot multiplication. Optimal rooting was achieved in shoots cultured on ½ strength MS medium supplemented with 5 μM each of IBA and NAA. Regenerated plantlets were acclimatized and hardened in green house using dune sand and vermi-compost (3:1) with 92.34% success and transferred to the field with 100% survival rate. In the field, plants supplied with FYM along with urea showed better growth and development. Macroproliferation, plant multiplication by separating the rooted tillers of well established in vitro raised plantlets after 5 to 6 months of growth in the green house could double the multiplication rate. More than 25000 in vitro raised plants were successfully transferred to the field and no morphological variations in growth were observed, thus proving the potential of tissue culture for raising large scale plantations of D. asper.

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Abbreviations

AdS:

Adenine sulfate

BAP:

6-benzyl-amino-purine

IBA:

Indole-3-butyric acid

KIN:

Kinetin

MS:

Murashige and Skoog

NAA:

α-naphthalene acetic acid

FYM:

Farm yard manure

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Acknowledgements

Research grant from Department of Biotechnology, Ministry of Science and Technology, Govt. of India, New Delhi, under bamboo mission project no. BT/PR/5261/AGR/16/459/2004, is gratefully acknowledged. The assistance provided by the scientific and technical staff of CPB is also acknowledged. The Forest Department, Haryana, India is acknowledged for providing the land for plantation as well as for maintenance of plants in the field.

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  1. A. K. Dhawan

    Present address: CCS HAU Regional Research Station, Uchani, Karnal, 132001, India

Authors and Affiliations

  1. Centre for Plant Biotechnology, CCS HAU New Campus, Hisar, 125004, Haryana, India

    Sharbati R. Singh, Rohtas Singh, A. K. Dhawan & Rajwant K. Kalia

  2. Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India

    Sharbati R. Singh & Sunita Dalal

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  1. Sharbati R. Singh
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Correspondence to Rajwant K. Kalia.

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Singh, S.R., Dalal, S., Singh, R. et al. Micropropagation of Dendrocalamus asper {Schult. & Schult. F.} Backer ex k. Heyne): an exotic edible bamboo. J. Plant Biochem. Biotechnol. 21, 220–228 (2012). https://doi.org/10.1007/s13562-011-0095-9

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