Abstract
Heavy metal toxicity is one of the major abiotic stresses caused by physiological and biochemical changes. Plants have evolved various phytochemical defense mechanisms to cope with this abiotic stress conditions. Phenolic compounds are one of the stress responses and have multiple roles in respect to adaptation of plants to the environment. In the present study, we aimed to evaluate the differential accumulation of various phenolics with HPLC in the leaves of corn exposed to increasing heavy metal doses in the plant growth medium. The application of Cd, Cu, and Pb increased the total phenolics in all treatments compared to control groups. Chlorogenic acid and rutin were the main phenolic compounds in respect to quantifying. However, the contents of caffeic acid, ferulic acid, and vanillic acid were comparatively lower than chlorogenic acid and rutin in all samples. The content of chlorogenic acid significantly increased and rutin slightly increased in the treatment of the heavy metals. The levels of caffeic acid and ferulic acid significantly decreased in all exposures of heavy metals compared to control groups. The content of vanillic acid changed according to heavy metal types and doses in the leaves of corn, and the low doses of Pb and Cd increased the level of vanillic acid. We show that there is a positive correlation with the total phenolic content and chlorogenic acid when the corn is exposed to Pb. Moreover, there are negative correlations between total phenolic compound and caffeic acid, ferulic acid in the application of Cu and Cd.
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References
Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99:191–203
Bubna GA, Lima RB, Zanardo DYL, Santos WD, Ferrarese MLL, Ferrarese-Filho O (2011) Exogenous caffeic acid inhibits the growth and enhances the lignification of the roots of soybean (Glycine max). J Plant Physiol 168:1627–1633
Cai Y, Lua Q, Sun M, Corke H (2004) Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 74:2157–2184
Calixto-Campos C, Carvalho TT, Hohmann MSN, Pinho-Ribeiro FA, Fattori V, Manchope MF, Zarpelon AC, Baracat MM, Georgetti SR, Casagrande R, Verri WA (2015) Vanillic acid inhibits inflammatory pain by inhibiting neutrophil recruitment, oxidative stress, cytokine production, and NFκB activation in mice. J Nat Prod 78:1799–1808
Cervilla LM, Blasco B, Rios JJ, Rosales MA, Sanchez-Rodriguez E, Rubio-Wilhelmi MM, Romero L, Ruiz JM (2012) Parameters symptomatic for boron toxicity in leaves of tomato plants. J Bot. doi:10.1155/2012/726206
Cheynier V (2012) Phenolic compounds: from plants to foods. Phytochem Rev 11:153–177
Chung IM, Kim JJ, Lim JD, Yu CY, Kim SH, Hahn SJ (2006) Comparison of resveratrol, SOD activity, phenolic compounds and free amino acids in Rehmannia glutinosa under temperature and water stress. Environ Exp Bot 56:44–53
Cseke LJ, Kirakosyan A, Kaufman PB, Warber SL, Duke JA, Brielmann HL (2006) Natural products from plants. Taylor and Francis, New York
Demirezen D, Aksoy A (2006) Heavy metal levels in vegetables in Turkey are within safe limits for Cu, Zn, Ni and exceeded for Cd and Pb. J Food Chem 29:252–265
Elguera JCT, Barrientos EY, Wrobel K, Wrobel K (2013) Effect of cadmium (Cd(II)), selenium (Se(IV)) and their mixtures on phenolic compounds and antioxidant capacity in Lepidium sativum. Acta Physiol Plant 35:431–441
Erenler R, Telci I, Ulutas M, Demirtas I, Gul F, Elmastas M, Kayır O (2015) Chemical constituents, quantıtatıve analysıs and antioxidant activities of echinacea purpurea (L.) moench and Echinacea pallıda (Nutt.) Nutt. J Food Biochem 39:622–630
Garcia-Sanchez M, Garrido I, Casimiro IJ, Casero PJ, Espinosa F, Garcia-Romera I, Aranda E (2012) Defence response of tomato seedlings to oxidative stress induced by phenolic compounds from dry olive mill residue. Chemosphere 89:708–716
Gonçalves JF, Becker AG, Cargnelutti D, Tabaldi L, Pereira LB, Battisti V, Spanevello RM, Morsch VM, Nicoloso FT, Schetinger MRC (2007) Cadmium toxicity causes oxidative stress and induces response of the antioxidant system in cucumber seedlings. Braz J Plant Physiol 19(3):223–232
Gratao PL, Polle A, Lea PL, Azevedo RA (2005) Making the life of heavy metal-stressed plants a little easier. Funct Plant Biol 32:481–494
Hassan M, Mansoor S (2014) Oxidative stress and defense mechanism in mung bean seedlings after lead and cadmium treatments. Turk J Agric For 38:55–61
Kovacik J, Gruz J, Backor M, Tomko J, Strnad M, Repcak M (2008) Phenolic compounds composition and physiological attributes of Matricaria chamomilla grown in copper excess. Environ Exp Bot 62:145–152
Kovacik J, Klejdus B, Hedbayny J, Stork F, Backor M (2009) Comparison of cadmium and copper effect on phenolic metabolism, mineral nutrients and stress-related parameters in Matricaria chamomilla plants. Plant Soil 320:231–242
Kovacık J, Klejdus B, Backor M (2009a) Phenolic metabolism of Matricaria chamomilla plants exposed to nickel. J Plant Physiol 166:1460–1464
Kovacık J, Klejdus B, Hedbavny J, Backor M (2009b) Salicylic acid alleviates NaCl-induced changes in the metabolism of Matricaria chamomilla plants. Ecotoxicology 18:544–554
Kumar N, Pruthi V (2014) Potential applications of ferulic acid from natural sources. Biotechnol Rep 4:86–93
Lequeux H, Hermans C, Lutss S, Verbruggen N (2010) Response to copper excess in Arabidopsis thaliana: impact on the root system architecture, hormone distribution, lignin accumulation and mineral profile. Plant Physiol Biochem 48:673–682
Marguez-Garcia B, Fernandez-Angeles M, Cordoba F (2009) Phenolics composition in Erica sp. differentially exposed to metal pollution in the Iberian Southwestern Pyritic Belt. Bioresour Technol 100(1):446–451
Marguez-Garcia B, Fernandez-Recamales MA, Cordoba F (2012) Effects of cadmium on phenolic composition and antioxidant activities of Erica andevalensis. J Bot. doi:10.1155/2012/936950
Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 15:523–530
Musallam I, Duwayri M, Shibli R, Alali F (2012) Investigation of rutin content indifferent plant parts of wild cape (Capparis spinosa L.) populations from Jordan. Res J Med Plant 6(1):27–36
Nazar R, Iqbal N, Masood A, Khan MIR, Syeed S, Khan NA (2012) Cadmium toxicity in plants and role of mineral nutrients in its alleviation. Am J Plant Sci 3:1476–1489
Ncube EN, Mhlongo M, Piater LA, Steenkamp PA, Dubery IA, Madala NE (2014) Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. Chem Cent J 8:66
Petridis A, Therios I, Samouris G, Koundoursa S (2012) Effect of water deficit on leaf phenolic composition, gas exchange, oxidative damage and antioxidant activity of four Greek olive (Olea europaea L.) cultivars. Plant Physiol Biochem 60:1–11
Racchi ML (2013) Antioxidant defenses in plants with attention to Prunus and Citrus spp. Antioxidants 2:340–369
Ramakrishna AR, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 6(11):1720–1731
Ramos-Escudero F, Munoz AM, Alvarado-Ortiz C, Alvarado A, Yanez JA (2012) Purple corn (Zea mays L.) phenolic compounds profile and its assessment as an agent against oxidative stress in isolated mouse organs. J Med Food 15:206–215
Rastgoo L, Alemzadeh A (2011) Biochemical responses of Gouan (Aeluropus littoralis) to heavy metals stress. Aust J Crop Sci 5(4):375–383
Rauha JP, Remes S, Heinonen M, Hopia A, Kahkönen M, Kujala T, Pihlaja K, Vuorela H, Vuorela P (2000) Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int J Food Microbiol 56:3–22
Rellan-Alvarez R, Ortega-Villasante C, Alvarez-Fernandez A (2006) Stress responses of Zea mays to cadmium and mercury. Plant Soil 279:41–50
Schützendübel A, Polle A (2002) Plant responses to abiotic stress: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351–1365
Sharma P, Dubey RS (2004) Lead toxicity in plants. Braz Plant Physiol 17:35–52
Singleton VL, Slinkard K (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 28:49–55
Tattini M, Galardi C, Pinelli P, Massai R, Remorini D, Agati G (2004) Differential accumulation of flavonoids and hydroxycinnamates in leaves of Ligustrum vulgare under excess light and drought stress. New Phytol 163:547–561
Tomas-Barberan FA, Espin JC (2001) Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J Sci Food Agric 81:853–876
Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655
Vermerris W, Nicholson R (2006) Phenolic compound biochemistry. Springer, Dordrecht
Wojdylo A, Oszmianski J, Czemerys R (2007) Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 105:940–949
Yadav SK (2010) Heavy metals toxicity in plants: an overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. S Afr J Bot 76:167–179
Zheng W, Wang SY (2001) Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 49:5165–5170
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The authors are most grateful to the Plant Research Laboratory (BALAB) of Chemistry Department at the Gaziosmanpasa University, which provided us with the HPLC unit and had been accommodating us during our experimentation.
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Kısa, D., Elmastaş, M., Öztürk, L. et al. Responses of the phenolic compounds of Zea mays under heavy metal stress. Appl Biol Chem 59, 813–820 (2016). https://doi.org/10.1007/s13765-016-0229-9
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DOI: https://doi.org/10.1007/s13765-016-0229-9