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Effect of Bacillus subtilis Strain CKT1 as Inoculum on Growth of Tomato Seedlings Under Net House Conditions

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The use of plant growth promoting rhizobacteria (PGPR) is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides, and supplements. The authors have isolated and characterized different PGPRs from the rhizosphere soils/roots of tomato with multiple plant growth promoting activities. Subsequently, to investigate the effects of PGPR isolates on the growth of tomato, a pot culture experiment was conducted under net house. Most of the isolates resulted in a significant increase in shoot length, root length and dry matter production of shoot and root of tomato seedlings. Among eleven isolates, N11 exhibited concomitant production of all plant growth promoting activities viz., phosphate solubilization, siderophore production, indole-acetic acid production including hydrogen cyanide production. Remarkable increase was observed in seed germination (36.08 %), shoot length (5.22 %), root length (21.12 %), shoot dry weight (63.50 %) and root dry weight (54.08 %), nitrogen (18.75 %), potassium (57.69 %) and phosphorus (22.22 %). Morphological, biochemical and 16S rRNA gene analysis identified strain N11 as Bacillus subtilis strain CKT1. The present study, therefore, suggests that the use of single strain inoculum of CKT1 with multiple plant growth promoting activities offers a recent concept to address multiple mode of action by combined use of multi-strain inoculum of PGPR with each having specific capability and function.

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Authors and Affiliations

  1. Microbiology Section, Department of Basic Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, 173230, HP, India

    Abhishek Walia & Anjali Chauhan

  2. Department of Biotechnology, National Institute of Plant Genomic Research, New Delhi, 110001, India

    Preeti Mehta

  3. Department of Basic Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, 173230, HP, India

    C. K. Shirkot

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  1. Abhishek Walia
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  2. Preeti Mehta
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  3. Anjali Chauhan
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  4. C. K. Shirkot
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Correspondence to C. K. Shirkot.

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Walia, A., Mehta, P., Chauhan, A. et al. Effect of Bacillus subtilis Strain CKT1 as Inoculum on Growth of Tomato Seedlings Under Net House Conditions. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 145–155 (2014). https://doi.org/10.1007/s40011-013-0189-3

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  • DOI: https://doi.org/10.1007/s40011-013-0189-3

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