Skip to main content

Advertisement

SpringerLink
Log in

Mycorrhization and phosphorus may be an alternative for increasing the production of metabolites in Myracrodruon urundeuva

  • Published:
Theoretical and Experimental Plant Physiology Aims and scope Submit manuscript

Abstract

‘Aroeira-do-sertão’ (Myracrodruon urundeuva Allemão; Anacardiaceae) is one of the most-used medicinal species by the population of northeastern Brazil and is on the list of plants threatened with extinction. In this context, alternatives that could contribute to reduced extractive use must be designed to establish crops of this species, which have high concentrations of metabolites. Inoculation with arbuscular mycorrhizal fungi (AMF) and the addition of phosphorus (P) to soil increases the production of primary and secondary metabolites in some plants. We tested the hypothesis that the effects of mycorrhization in ‘aroeira’ can be maximized by the application of P. The dry matter and the concentration of metabolites were evaluated. Mycorrhization increased the accumulation of phytomass, which was also favored at the highest dosage of P tested. The production of phenols and total proteins was maximized by mycorrhization only at an intermediate dosage of P. The inoculation did not favor the synthesis of flavonoids and soluble carbohydrates. The production of proanthocyanidins was influenced only by phosphate supplementation. It is concluded that the mycorrhization of seedlings of ‘aroeira-do-sertão’ associated with P fertilization maximizes the production of total phenols and total foliar proteins, making the phytomass more attractive to the herbal industry and resulting in the use of P fertilization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Albuquerque UP, Andrade LHC (2002) Uso de recursos vegetais da Caatinga: o caso do agreste do estado de Pernambuco (nordeste do Brasil). Interciência 27:336–346

    Google Scholar 

  • Alves PM, Queiroz LMG, Pereira JV, Pereira MSV (2009) Atividade antimicrobiana, antiaderente e antifúngica in vitro de plantas medicinais brasileiras sobre microrganismos do biofilme dental e cepas do gênero Candida. Rev Soc Bras Med Trop 42:222–224

    Article  PubMed  Google Scholar 

  • Araújo TAS, Alencar NL, Amorim ELC, Albuquerque UP (2008) A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. J Ethnopharmacol 120:72–80

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Brito HO, Noronha EP, França LM, Brito LMO, Prado SA (2008) Análise da composição fitoquímica do extrato etanólico das folhas de Annona squamosa (ATA). Rev Bras Farm 89:180–184

    CAS  Google Scholar 

  • Castellanos-Morales V, Villegas J, Wendelin S, Vierheilig H, Eder R, Cárdenas-Navarro R (2010) Root colonization by the arbuscular mycorrhizal fungus Glomus intraradices alters the quality of strawberry fruits (Fragaria x ananassa Duch.) at different nitrogen levels. J Sci Food Agric 90:1774–1782

    CAS  PubMed  Google Scholar 

  • Dubois M, Guiles A, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–355

    Article  CAS  Google Scholar 

  • Freitas MSM, Carvalho AJC, Carneiro RFV (2004) Crescimento e produção de fenóis totais em carqueja [Baccharis trimera (Less.) DC] em resposta a inoculação com fungos micorrízicos arbusculares, na presença e na ausência de adubação mineral. Rev Bras Plantas Med 6:30–34

    CAS  Google Scholar 

  • Geneva MP, Stancheva IV, Boychinova MN, Mincheva NH, Yonova PA (2010) Effects of foliar fertilization and arbuscular mycorrhizal colonization on Salvia officinalis L. growth, antioxidant capacity, and essential oil composition. J Sci Food Agric 90:696–702

    CAS  PubMed  Google Scholar 

  • Karagiannidis N, Thomidis T, Lazari D, Panou-Filotheou E, Karagiannidou C (2011) Effect of three Greek arbuscular mycorrhizal fungi in improving the growth, nutrient concentration, and production of essential oils of oregano and mint plants. Sci Hortic 129:329–334

    Article  CAS  Google Scholar 

  • Kill LHP, Lima PCF (2011) Plano de manejo para espécies da Caatinga ameaçadas de extinção na reserva legal do projeto Salitre. Embrapa Semiárido. Documentos: 243, 55pp

  • Lima CS, Campos MAS, Silva FSB (2015) Mycorhizal fungi (AMF) increase the content of biomolecules in leaves of Inga vera Willd. seedlings. Symbiosis 65:117–123

    Article  CAS  Google Scholar 

  • Mandal S, Upadhyay S, Wajid S, Ram M, Jain DC (2015) Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels. Mycorrhiza 25:345–357

    Article  CAS  PubMed  Google Scholar 

  • Middleton E Jr, Kandaswami C, Theoharides TC (2000) The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 52:673–751

    CAS  PubMed  Google Scholar 

  • Monteiro JM, Albuquerque UP, Araújo EL (2005) Taninos: uma abordagem da química à ecologia. Quím. Nova 28:892–896

    Article  CAS  Google Scholar 

  • Monteiro JM, Albuquerque UP, Lins Neto EMF, Araújo EL, Albuquerque MM, Amorim ELC (2006) The effects of seasonal climate changes in the Caatinga on tannin levels in Myracrodruon urundeuva (Engl.) Fr. All. and Anadenanthera colubrine (Vell.) Brenan. Braz J Pharmacogn 16:338–344

    Article  CAS  Google Scholar 

  • Monteiro JM, Araújo EL, Amorim ELC, Albuquerque UP (2012) Valuation of the Aroeira (Myracrodruon urundeuva Allemão): perspectives on conservation. Acta Bot Bras 26:125–132

    Article  Google Scholar 

  • Oliveira AS, Morais MLT, Buzti S (2002) Aspectos nutricionais da variação genética em progênies de Myracrodruon urundeuva Fr. All. sob diferentes condições de cultivo. Floresta 29:3–14

    Google Scholar 

  • Oliveira MS, Campos MAS, Albuquerque UP, Silva FSB (2013) Arbuscular mycorrhizal fungi (AMF) affects biomolecules content in Myracrodruon urundeuva seedlings. Ind Crop Prod 50:244–247

    Article  CAS  Google Scholar 

  • Oliveira MS, Campos MAS, Silva FSB (2015) Arbuscular mycorrhizal fungi and vermicompost to maximize the production of foliar biomolecules in Passiflora alata Curtis seedlings. J Sci Food Agric 95:522–528

    Article  CAS  PubMed  Google Scholar 

  • Pedone-Bonfim MVL, Lins MA, Coelho IR, Santana A, Silva FSB, Maia LC (2013) Mycorrhizal technology and phosphorus in the production of primary and secondary metabolites in cebil (Anadenanthera colubrina (Vell.) Brenan) seedlings. J Sci Food Agric 93:1479–1484

    Article  CAS  PubMed  Google Scholar 

  • Pedone-Bonfim MVL, Silva FSB, Maia LC (2015) Production of secondary metabolites by mycorrhizal plants with medicinal or nutritional potential. Acta Physiol Plantarum 37:27–33

    Article  CAS  Google Scholar 

  • Ponce MA, Bompadre MJ, Scervino JM, Ocampo JÁ, Chaneton EJ, Godeas AM (2009) Flavonoids, benzoic acids and cinnamic acids isolated from shoots and roots of Italian rye Grass (Lolium multiflorum Lam.) with and without endophyte association and arbuscular mycorrhizal fungus. Biochem Syst Ecol 37:245–253

    Article  CAS  Google Scholar 

  • Queiroz CRAA, Morais SAL, Nascimento EA (2002) Caracterização dos taninos da aroeira-preta (Myracrodruon urundeuva). Rev Árvore 26:485–492

    Article  CAS  Google Scholar 

  • Santos SC, Mello JCP (2007) Taninos. In: Schenkel CMO, Gosmann EP, Mell G, Mentz JCP, Petrovick LA, Simões PR (eds) Farmacognosia: da planta ao medicamento. UFRGS, Porto Alegre, pp 615–656

    Google Scholar 

  • Silva ACO, Albuquerque UP (2005) Woody medicinal plants of the caatinga in the state of Pernambuco (Northeast Brasil). Acta Bot Bras 19:17–26

    Article  Google Scholar 

  • Silva FA, Silva FSB (2017) Is the application of arbuscular mycorrhizal fungi an alternative to increase foliar phenolic compounds in seedlings of Mimosa tenuiflora Wild) Poir., Mimosoideae? Braz J Bot 40:361–365

    Article  Google Scholar 

  • Silva FA, Ferreira MRA, Soares LAL, Sampaio EVSB, Silva FSB, Maia LC (2014a) Arbuscular mycorrhizal fungi increase gallic acid production in leaves of field grown Libidibia ferrea (Mart. ex. Tul.) L. P. Queiroz. J Med Pl Res 8:1110–1115

    Article  CAS  Google Scholar 

  • Silva LG, Martins LMV, Silva FSB (2014b) Arbuscular mycorrhizal symbiosis in the maximization of the concentration of foliar biomolecules in pomegranate (Punica granatum L.) seedlings. J Med Pl Res 8:953–957

    Article  CAS  Google Scholar 

  • Souza SMC, Aquino LCM, Milach AC Jr, Bandeira MAM, Nobre MEP, Viana GSB (2007) Antiinflammatory and antiulcer properties of tannins from Myracrodruon urundeuva Allemão (Anacardiaceae) in rodents. Phytother Res 21:220–225

    Article  CAS  PubMed  Google Scholar 

  • Tawaraya K, Hirose R, Wagatsuma T (2012) Inoculation of arbuscular mycorrhizal fungi can substancially reduce phoshate fertilizer application to Allium fistulosum L. and achieve marketable yield under field conditions. Biol Fertil Soils 48:839–843

    Article  Google Scholar 

  • Toussaint JP, Smith FA, Smith SE (2007) Arbuscular mycorrhizal fungi can induce the production of phytochemicals in sweet basil irrespective of phosphorus nutrition. Mycorrhiza 17:291–297

    Article  CAS  Google Scholar 

  • Viana GSB, Bandeira MAM, Matos FJA (2003) Analgesic and antiinflammatory effects of chalcones isolated from Myracrodruon urundeuva Allemão. Phytomedicine 10:189–195

    Article  CAS  PubMed  Google Scholar 

  • Zimare SB, Borde MY, Jite PK, Malpathak NP (2013) Effect of AM fungi (Gf, Gm) on biomass and gymnemic content of Gymnema sylvestre (Retz.) R. Br. ex Sm. Proc Natl Acad Sci India Sect B: Biol Sci 83:439–445

    Article  CAS  Google Scholar 

  • Zubek S, Mielcarek S, Turnau K (2012) Hypericin and pseudohypericin concentrations of a valuable medicinal plant Hypericum perforatum L. are enhanced by arbuscular mycorrhizal fungi. Mycorrhiza 22:149–156

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support (Edital Universal, Proc. No. 473779/2011-0) and for productivity research grants (Proc. Nos. 307749/2015-0 and 307129/2015-2).

Author information

Authors and Affiliations

  1. Laboratório de Análises, Pesquisas e Estudos em Micorrizas, Centro de Pesquisas do Instituto de Ciências Biológicas/Universidade de Pernambuco, Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada, Instituto de Ciências Biológicas – ICB/UPE, Rua Arnóbio Marques, 310, Santo Amaro, Recife, PE, 50100-130, Brazil

    Fábio Sérgio Barbosa da Silva

  2. Departamento de Micologia, Programa de Pós-Graduação em Biologia de Fungos, Universidade Federal de Pernambuco, Rua Nelson Chaves, s/n, Recife, PE, 50670-420, Brazil

    Leonor Costa Maia

Authors
  1. Fábio Sérgio Barbosa da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leonor Costa Maia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fábio Sérgio Barbosa da Silva.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

da Silva, F.S.B., Maia, L.C. Mycorrhization and phosphorus may be an alternative for increasing the production of metabolites in Myracrodruon urundeuva. Theor. Exp. Plant Physiol. 30, 297–302 (2018). https://doi.org/10.1007/s40626-018-0123-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40626-018-0123-4

Keywords

Search

Navigation