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Management of root-knot nematode in cucumber through seed treatment with multifarious beneficial microbes in polyhouse under protected cultivation

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Abstract

The plants of cucumber, Cucumis sativus cv. Green Long grown in earthen pots filled with nematode infected soil (1500 juveniles of Meloidogyne incognita/kg soil) without any treatment under polyhouse conditions showed stunted growth with mild chlorosis of the leaves and excessive galling on the roots. Seed treatment with Pseudomonas fluorescens ITCC-B0034, Bacillus subtilis RP24, Trichoderma harzianum ITCC-6796, T. longibrachiatum ITCC-7437, T. viride ITCC-6889, and nematicide (carbofuran) decreased the galling (16–68%), egg mass production (12–62%), and the nematode soil population (29–87%) over untreated control. Maximum decline in the galls/root system was recorded with carbofuran (68%), T. viride (66%) and T. harzianum (61%). The above treatments caused around 55–62% suppression in the egg mass count. The root zone population of M. incognita was decreased by 87% (carbofuran) and 79–84% (T. viride and T harzianum) over control. Treatments with carbofuran, T. viride and T. harzianum significantly enhanced the plant growth of nematode infected cucumber plants (15–28%), followed by P. fluorescens (13–20%) over inoculated control. Treatment with P. fluorescens also significantly enhanced the growth of uninoculated plants. Nematode inoculation decreased the plant biomass (28%), leaf chlorophyll a (24%), chlorophyll b (20%), total chlorophyll (25%), carotenoids (27%), and photosynthesis rate (23%), and increased the transpiration rate (18%) over control. The above biochemical and physiological parameters increased by 5–17% in nematode infected plants receiving T. viride, T. harzianum or carbofuran treatments.

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  1. Department of Plant Protection, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002, India

    Rafeeq Kasim Abdu Mohammed & Mujeebur Rahman Khan

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  1. Rafeeq Kasim Abdu Mohammed
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  2. Mujeebur Rahman Khan
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Correspondence to Mujeebur Rahman Khan.

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Mohammed, R.K.A., Khan, M.R. Management of root-knot nematode in cucumber through seed treatment with multifarious beneficial microbes in polyhouse under protected cultivation. Indian Phytopathology 74, 1035–1043 (2021). https://doi.org/10.1007/s42360-021-00422-3

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