Abstract
The transmitting-tissue cells of the style of flowering plants secrete a complex extracellular matrix through which pollen tubes grow to the ovary to effect fertilisation. This matrix is particularly rich in a class of proteoglycans, the arabinogalactan-proteins (AGPs). AGPs from the ovary of Nicotiana alata were found to be developmentally regulated, as the different charge classes of AGPs altered during floral development. The AGPs from the mature ovary had charge characteristics that were distinct from those previously reported for the stigma and style. However, the concentration of AGP (0.6 μg/ml fresh weight) in the ovary did not change during development, or in response to either compatible or incompatible pollination. The AGPs of the ovary are mainly associated with the epidermis of the placenta.
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Anderson MA, Cornish EC, Mau S-L, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roche PJ, Haley JD, Penschow JD, Niall HD, Tregear G, Coghlan JP, Crawford RJ, Clarke AE (1986) Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38–44
Chen C-G, Cornish EC, Clarke AE (1992) Specific expression of an extensin-like gene in the style of Nicotiana alata. Plant Cell 4:1053–1062
Chen C-G, Pu Z-Y, Moritz RL, Simpson RJ, Bacic A, Clarke AE, Mau S-L (1994) Molecular cloning of a gene encoding an arabinogalactan-protein from pear (Pyrus communis) cell suspension culture. Proc Natl Acad Sci USA 91:10305–10309
Clarke AE, Gleeson PA, Jermyn MA, Knox RB (1978) Characterization and localization of β-lectins in lower and higher plants. Aust J Plant Physiol 5:707–722
Du H, Simpson RJ, Moritz RL, Clarke AE, Bacic A (1994) Isolation of the protein backbone of an arabinogalactan-protein from the styles of Nicotiana alata and characterisation of a corresponding cDNA. Plant Cell 6:1643–1653
Fincher GB, Stone BA, Clarke AE (1983) Arabinogalactan-proteins: structure, biosynthesis and function. Ann Rev Plant Physiol 34:47–70
Gane AM (1994) The extracellular matrix and cell walls of pistils of Nicotiana alata. PhD thesis, University of Melbourne, Melbourne, Australia
Gane A, Craik D, Munro SLA, Hewlett GJ, Clarke AE, Bacic A (1995) Structural analysis of (the carbohydrate moiety of) arabinogalacton-proteins from stigmas and styles of Nicotiana alata. Carbohydr Res
Gell AC, Bacic A, Clarke AE (1986) Arabinogalactan-proteins of the female sexual tissue of Nicotiana alata. I. Changing during flower development and pollintion. Plant Physiol 82:885–889
Gleeson PA, Clarke AE (1979) Structural studies on the major component of Gladiolus style mucilage, an arabinogalactanprotein. Biochem J 181:607–621
Gleeson PA, Clarke AE (1980) Arabinogalactans of sexual and somatic tissues of Gladiolus and Lilium. Phytochemistry 19:1777–1782
Hoggart RM, Clarke AE (1984) Arabinogalactans are common components of Angiosperm styles. Phytochemistry 23:1571–1574
Holst G-J van, Clarke AE (1985) Quantification of arabinogalactan-protein in plant extracts by single radial gel diffusion. Anal Biochem 148:446–450
Hoist G-J van, Clarke AE (1986) Organ-specific arabinogalactanproteins of Lycopersicon peruvianum (Mill.) demonstrated by crossed electrophoresis. Plant Physiol 80:786–789
Jermyn MA (1978) Comparative specificity of concanavalin A and β-lectins. Aust J Plant Physiol 5:687–696
Knox JP (1992) Cell adhesion, cell separation and plant morphogenesis. Plant J 2:137–141
Knox RB (1984) Pollen-pistil interactions. In: Linskens HE, Heslop-Harrison J (eds) Cellular interactions (Encyclopaedia of plant physiology, NS, vol 17) Springer, Berlin Heidelberg New York, pp 508–608
Labarca C, Loewus FA (1972) The nutritional role of pistil exudate in pollen tube wall formation in Lilium longiflorum. I. Utilization of injected stigma exudate. Plant Physiol 50:7–14
Labarca C, Loewus FA (1973) The nutritional role of pistil exudate in pollen tube wall formation in Lilium longiflorum. II. Production and utilization of exudate from the stigma and style canal. Plant Physiol 52:87–92
Lind JL, Bacic A, Clarke AE, Anderson MA (1994) A style-specific hydroxyprolinerich glycoprotein has properties of both extensin and arabinogalactan proteins. Plant J 6:491–502
Penneil RI (1992) Cell surface arabinogalactan proteins and arabinogalactans and plant development. In: Callow JA, Green JR (eds) Perspectives in plant cell recognition (Society for Experimental Biology Seminar Series 48) Cambridge University Press, Cambridge UK, pp 105–121
Pennell RI, Janniche L, Kjellbom P, Scofield GN, Peart JM, Roberts K (1991) Development regulation of a plasma membrane arabinogalactan protein epitope in oilseed rape flowers. Plant Cell 3:1317–1326
Sanders LC, Lord EM (1989) Directed movement of latex particles in the gynoecia of three species of flowering plants. Science 243:1606–1608
Sanders LC, Lord EM (1992) A dynamic role for the stylar matrix in pollen tube extension. Int Rev Cytol 140:297–318
Sanders LC, Wang C-O, Walling LL, Lord EM (1991) A homolog of the substrate adhesion molecule vitronectin in four species of flowering plants. Plant Cell 3:629–635
Sedgley M, Clarke AE (1986) Immuno-gold localisation of arabinogalactan protein in the developing style of Nicotiana alata. Nord J Bot 6:591–598
Sedgley M, Blesing MA, Bonig I, Anderson MA, Clarke AE (1985) Arabinogalactanproteins are localised extracellularly in the transmitting tissue of Nicotiana alata Link and Otto, an ornamental tobacco. Micron Microsc Acta 16:247–254
Sokal RR, Rohlf FJ (1969) Biometry. Freeman, San Francisco
Webb MC, Williams EG (1988a) Effects of temperature, light, nutrients and carbon dioxide on the strength of the self-incompatibility response in detached flowers of Lycopersicon peruvianum. Ann Bot 61:395–404
Webb MC, Williams EG (1988b) The pollen tube pathway in the pistil of Lycopersicon peruvianum. Ann Bot 61:415–423
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Gane, A.M., Clarke, A.E. & Bacic, A. Localisation and expression of arabinogalactan-proteins in the ovaries of Nicotiana alata Link and Otto. Sexual Plant Reprod 8, 278–282 (1995). https://doi.org/10.1007/BF00229384
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DOI: https://doi.org/10.1007/BF00229384