Abstract.
Hordeum bulbosum represents the secondary gene pool of barley and constitutes a potential source of various disease resistances in barley breeding. Interspecific crosses of H. vulgare × H. bulbosum resulted in recombinant diploid-barley progeny with immunity to BaMMV after mechanical inoculation. Tests on fields contaminated with different viruses demonstrated that resistance was effective against all European viruses of the soil-borne virus complex (BaMMV, BaYMV-1, -2). Genetic analysis revealed that resistance was dominantly inherited. Marker analysis in a F5 mapping family was performed to map the introgression in the barley genome and to estimate its size after several rounds of recombination. RFLP anchor-marker alleles indicative of an H. bulbosum introgression were found to cover an interval 2.9 cM in length on chromosome 6HS. The soil-borne virus resistance locus harboured by this introgressed segment was designated Rym14 Hb. For marker-assisted selection of Rym14 Hb carriers, a diagnostic codominant STS marker was derived from an AFLP fragment amplified from leaf cDNA of homozygous-resistant genotypes inoculated with BaMMV.
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Acknowledgements.
R. Pickering thanks the Foundation for Research Science and Technology (New Zealand) for supporting part of this research under the Public Good Science Fund, and Professor Ingo Schubert (Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany) for helpful advice on FISH and allowing this work to be performed in his laboratory.
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Ruge, B., Linz, A., Pickering, R. et al. Mapping of Rym14 Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley. Theor Appl Genet 107, 965–971 (2003). https://doi.org/10.1007/s00122-003-1339-4
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DOI: https://doi.org/10.1007/s00122-003-1339-4