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Using molecular markers to assess the effect of introgression on quantitative attributes of common bean in the Andean gene pool

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Abstract

Progress in bean breeding programs requires the exploitation of genetic variation that is present among races or through introgression across gene pools of Phaseolus vulgaris L. Of the two major common bean gene pools, the Andean gene pool seems to have a narrow genetic base, with about 10% of the accessions in the CIAT core collection presenting evidence of introgression. The objective of this study was to quantify the degree of spontaneous introgression in a sample of common bean landraces from the Andean gene pool. The effects of introgression on morphological, economic and nutritional attributes were also investigated. Homogeneity analysis was performed on molecular marker data from 426 Andean-type accessions from the primary centres of origin of the CIAT common bean core collection and two check varieties. Quantitative attribute diversity for 15 traits was studied based on the groups found from the cluster analysis of marker prevalence indices computed for each accession. The two-group summary consisted of one group of 58 accessions (14%) with low prevalence indices and another group of 370 accessions (86%) with high prevalence indices. The smaller group occupied the outlying area of points displayed from homogeneity analysis, yet their geographic origin was widely distributed over the Andean region. This group was regarded as introgressed, since its accessions displayed traits that are associated with the Middle American gene pool: high resistance to Andean disease isolates but low resistance to Middle American disease isolates, low seed weight and high scores for all nutrient elements. Genotypes generated by spontaneous introgression can be helpful for breeders to overcome the difficulties in transferring traits between gene pools.

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Acknowledgements

This research was financially supported by a grant from the Australian Centre for International Agricultural Research (ACIAR), Canberra, ACT. The experiments and analyses reported in this paper comply with the current laws of both Colombia and Australia. Constructive comments from a reviewer and the Editor were very helpful.

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Authors and Affiliations

  1. Western Australian Institute for Medical Research, Australia, 6009, Nedlands, WA, Australia

    F. M. Amirul Islam

  2. Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia

    S. Beebe, M. Muñoz & J. Tohme

  3. Department of Natural Resources and Environment, Agriculture Victoria, 3401, Horsham, Vic, Australia

    R. J. Redden

  4. School of Land and Food Sciences, The University of Queensland, 4072, Brisbane, Qld, Australia

    K. E. Basford

Authors
  1. F. M. Amirul Islam
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  2. S. Beebe
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  3. M. Muñoz
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  4. J. Tohme
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  5. R. J. Redden
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  6. K. E. Basford
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Corresponding author

Correspondence to K. E. Basford.

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Communicated by H.C. Becker

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Amirul Islam, F.M., Beebe, S., Muñoz, M. et al. Using molecular markers to assess the effect of introgression on quantitative attributes of common bean in the Andean gene pool. Theor Appl Genet 108, 243–252 (2004). https://doi.org/10.1007/s00122-003-1437-3

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  • DOI: https://doi.org/10.1007/s00122-003-1437-3

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