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The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar

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Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici is a major disease of wheat (Triticum aestivum L.) that can be controlled by resistance breeding. The CIMMYT bread wheat line Saar is known for its good level of partial and race non-specific resistance, and the aim of this study was to map QTLs for resistance to powdery mildew in a population of 113 recombinant inbred lines from a cross between Saar and the susceptible line Avocet. The population was tested over 2 years in field trials at two locations in southeastern Norway and once in Beijing, China. SSR markers were screened for association with powdery mildew resistance in a bulked segregant analysis, and linkage maps were created based on selected SSR markers and supplemented with DArT genotyping. The most important QTLs for powdery mildew resistance derived from Saar were located on chromosomes 7DS and 1BL and corresponded to the adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29. A major QTL was also located on 4BL with resistance contributed by Avocet. Additional QTLs were detected at 3AS and 5AL in the Norwegian testing environments and at 5BS in Beijing. The population was also tested for leaf rust (caused by Puccinia triticina) and stripe rust (caused by P. striiformis f. sp. tritici) resistance and leaf tip necrosis in Mexico. QTLs for these traits were detected on 7DS and 1BL at the same positions as the QTLs for powdery mildew resistance, and confirmed the presence of Lr34/Yr18 and Lr46/Yr29 in Saar. The powdery mildew resistance gene at the Lr34/Yr18 locus has recently been named Pm38. The powdery mildew resistance gene at the Lr46/Yr29 locus is designated as Pm39.

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Acknowledgments

This work was supported by grants from the Norwegian Research Council, The International Collaboration Project from Ministry of Agriculture of China (2006-G2), and GRDC, Australia. The helpful assistance of Anne Guri Marøy with molecular marker genotyping and Yalew Tarkegne for the field trials is greatly acknowledged. We also thank Alireza Navabi for providing the seed of the Avocet × Saar population that was used to produce the F6 lines used in the study, and Martin Ganal at TraitGenetics Inc., Gatersleben, Germany and Atushi Torada at Hokuren Agricultural Research Institute, Hokkaido, Japan for providing unpublished primer sequences.

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432 , Ås, Norway

    M. Lillemo, B. Asalf & Å. Bjørnstad

  2. CIMMYT, Apdo. Postal 6-641, 06600, México, D.F., Mexico

    R. P. Singh

  3. Campo Experimental Valle de Mexico-INIFAP, Apdo. Postal 10, 56230, Chapingo, Edo. de Mexico, Mexico

    J. Huerta-Espino

  4. Institute of Crop Sciences/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Zhongguancun South Street 12, 100081, Beijing, People’s Republic of China

    X. M. Chen & Z. H. He

  5. CIMMYT China Office, c/o Chinese Academy of Agricultural Sciences, Zhongguancun South Street 12, 100081, Beijing, People’s Republic of China

    Z. H. He

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  1. M. Lillemo
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  2. B. Asalf
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Correspondence to M. Lillemo.

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Communicated by D. Mather.

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Lillemo, M., Asalf, B., Singh, R.P. et al. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116, 1155–1166 (2008). https://doi.org/10.1007/s00122-008-0743-1

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  • DOI: https://doi.org/10.1007/s00122-008-0743-1

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