Abstract
A mapping population of 186 recombinant inbred lines developed from a cross between UC1110, an adapted California spring wheat, and PI610750, a synthetic derivative from CIMMYT’s Wide Cross Program, was evaluated for its response to current California races of stripe rust (Puccinia striiformis f. sp. tritici) in replicated field trials over four seasons (2007–2010) in the northern Sacramento Valley. A genetic map was constructed consisting of 1,494 polymorphic probes (SSRs, DArTs, and ESTs) mapped to 558 unique loci, and QTL analysis revealed the presence of four stripe rust resistance QTL segregating in this population, two from UC1110 (on chromosomes 3BS and 2BS) and two from PI610750 (5AL and 2AS). The two QTL of largest effects (on 3BS and 5AL) were validated in independent populations and their intervals narrowed to 2.5 and 5.3 cM, respectively. The 3BS QTL was shown, by allelism test and genotype, to carry a gene different from the Yr30/Sr2 complex. Mapped position also suggests that the 3BS QTL is associated with a gene different from either Yrns-B1 or YrRub, two stripe rust resistance genes mapped to this region in other studies. The 5AL QTL carries a previously unreported partial stripe rust resistance gene, designated here as Yr48. This paper discusses the individual contributions to resistance of these four QTL, their epistatic interactions, and their potential in durable resistance breeding strategies based on combinations of partial resistance genes.
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Notes
The reported fragment length in Carter et al. (2009) of 154 bp includes a 19-bp M13 tail, which was subtracted here for the sake of comparison.
Abbreviations
- AFLP:
-
Amplified fragment length polymorphism
- ANOVA:
-
Analysis of variance
- cM:
-
centiMorgan (in this population, Kosambi)
- DArT:
-
Diversity array technology
- EST:
-
Expressed sequence tag
- HTAP:
-
High-temperature adult plant
- LOD:
-
Log of the odds
- NIL:
-
Near-isogenic line
- PST:
-
Puccinia striiformis Westend. f. sp. tritici Eriks
- QTL:
-
Quantitative trait locus or quantitative trait loci, depending on context
- RIL:
-
Recombinant inbred line
- SSR:
-
Simple sequence repeat (microsatellite)
- 3BS:
-
The short arm of chromosome 3B
- 5AL:
-
The long arm of chromosome 5A
- 2BS:
-
The short arm of chromosome 2B
- 2AS:
-
The short arm of chromosome 2A
References
Bansal U, Hayden M, Gill M, Bariana H (2010) Chromosomal location of an uncharacterised stripe rust resistance gene in wheat. Euphytica 171:121–127
Bariana H, Parry N, Barclay I, Loughman R, McLean R, Shankar M, Wilson R, Willey N, Francki M (2006) Identification and characterization of stripe rust resistance gene Yr34 in common wheat. Theor Appl Genet 112:1143–1148
Basten CJ, Weir BS, Zeng Z-B (2004) QTL Cartographer Version 1.17. http://statgen.ncsu.edu/qtlcart
Börner A, Röder MS, Unger O, Meinel A (2000) The detection and molecular mapping of a major gene for non-specific adult-plant disease resistance against stripe rust (Puccinia striiformis) in wheat. Theor Appl Genet 100:1095–1099
Carter AH, Chen XM, Garland-Campbell K, Kidwell KK (2009) Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar ‘Louise’. Theor Appl Genet 119:1119–1128
Chen XM (2005) Epidemiology and control of stripe rust (Puccinia striiformis f. sp tritici) on wheat. Can J Plant Pathol 27:314–337
Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J Agr Res 58:648–655
Chen XM, Moore M, Milus EA, Long DL, Line RF, Marshall D, Jackson L (2002) Wheat stripe rust epidemics and races of Puccinia striiformis f. sp tritici in the United States in 2000. Plant Dis 86:39–46
Chen X, Soria MA, Yan G, Sun J, Dubcovsky J (2003) Development of user-friendly PCR markers for wheat stripe rust resistance gene Yr5. Crop Sci 43:2058–2064
Chen WQ, Wu LR, Liu TG, Xu SC, Jin SL, Peng YL, Wang BT (2009) Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101
Chen X, Penman L, Wan A, Cheng P (2010) Virulence races of Puccinia striiformis f. sp. tritici in 2006 and 2007 and development of wheat stripe rust and distributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States. Can J Plant Pathol 32:315–333
Cheng P and Chen X (2008) Molecular mapping of a gene for resistance to stripe rust in spring wheat cultivar IDO377s. APS Annual Meeting. Phyto 98:S38
Dimmock JPRE, Gooding MJ (2002) The influence of foliar diseases, and their control by fungicides, on the protein concentration in wheat grain: a review. J Agric Sci 138:349–366
Distelfeld A, Tranquilli G, Li C, Yan L, Dubcovsky J (2009) Genetic and molecular characterization of the VRN2 loci in tetraploid wheat. Plant Phys 149:245–257
Hodson D, Nazari K (2010) “Serious outbreaks of wheat stripe or yellow rust in Central and West Asia and North Africa.” Borlaug Global Rust Initiative. http://www.globalrust.org/traction?type=single&proj=Pathogen&sort=2&rec=206. Accessed June 2010
Hovmøller MS, Yahyaoui AH, Milus EA, Justesen AF (2008) Rapid global spread of two aggressive strains of a wheat rust fungus. Mol Ecol 17:3818–3826
Hovmøller MS, Walter S, Justesen AF (2010) Escalating threat of wheat rusts. Science 329:369
Jackson LF, Dubcovsky J, Gallagher LW, Chicaiza O, Stewart D, Gibbs LK, Prato-Mayo D, Kirby D, Carlson H, Canevari M, Marsh B, Meister H, Munier D, Orloff S, Roberts B, Schmierer J, Vargas R, Wilson R, Wright S (2003) 2003 Regional barley, common and durum wheat, triticale, and oat performance tests in California. Cal Coop Ext Agr Prog Rep 286. http://agric.ucdavis.edu/crops/cereals/2003/oct2003_apr286.htm. Accessed June 2010
Khlestkina E, Röder M, Unger O, Meinel A, Börner A (2007) More precise map position and origin of a durable non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) in wheat. Euphytica 153:1–10
Kota R, Spielmeyer W, McIntosh RA, Lagudah ES (2006) Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo-black chaff in common wheat (Triticum aestivum L.). Theor Appl Genet 112:492–499
Lincoln SE, Daly MJ, Lander ES (1993) MAPMAKER/EXP Version 3.0. http://linkage.rockefeller.edu/soft/mapmaker
Lowe I, Cantu D, Dubcovsky J (2011) Durable resistance to the wheat rusts: integrating systems biology and traditional phenotype-based research methods to guide the deployment of resistance genes. Euphytica doi:10.1007/s10681-010-0311-z
Luo PG, Ren ZL, Zhang HQ, Zhang HY (2005) Identification, chromosome location, and diagnostic markers for a new gene (YrCN19) for resistance to wheat stripe rust. Phytopathology 95:1266–1270
Mago R, Brown-Guedira G, Dreisigacker S, Breen J, Jin Y, Singh R, Appels R, Lagudah ES, Ellis J, Spielmeyer W (2011) An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat. Theor Appl Genet 122:735–744
Markell SG, Milus EA (2008) Emergence of a novel population of Puccinia striiformis f. sp. tritici in eastern United States. Phytopathology 98:632–639
McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2010) Catalogue Of Gene Symbols For Wheat: 2010 Supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2010.pdf. Accessed June 2010
Milus EA, Kristensen K, Hovmøller MS (2009) Evidence for increased aggressiveness in a recent widespread strain of Puccinia striiformis f. sp tritici causing stripe rust of wheat. Phytopathology 99:89–94
Mohler V, Lukman R, Ortiz-Islas S, William M, Worland A, van Beem J, Wenzel G (2004) Genetic and physical mapping of photoperiod insensitive gene Ppd-B1 in common wheat. Euphytica 138:33–40
Mujeeb-Kazi A, Gilchrist LI, Villareal RL, Delgado R (2000) Registration of ten wheat germplasm lines resistant to Septoria tritici leaf blotch. Crop Sci 40:590–591
Paux E, Sourdille P, Salse J, Saintenac C, Choulet F, Leroy P, Korol A, Michalak M, Kianian S, Spielmeyer W, Lagudah E, Somers D, Kilian A, Alaux M, Vautrin S, Berges H, Eversole K, Appels R, Safar J, Simkova H, Dolezel J, Bernard M, Feuillet C (2008) A physical map of the 1-gigabase bread wheat chromosome 3B. Science 322:101–104
Pu ZJ, Chen GY, Wei YM, Yang WY, Yan ZH, Zheng YL (2010) Identification and molecular tagging of a stripe rust resistance gene in wheat line P81. Plant Breed 129:53–57
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Sears ER (1954) The aneuploids of common wheat. Univ Mo Agric Exp Stn Res Bull 572:1–58
Sears ER, Steinitz-Sears LM (1978) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proceedings of the 5th International Wheat Genetics Symposium, New Delhi, 23–28 February 1988. Indian Society of Genetics and Plant Breeding, New Delhi, India. pp 389–407
Sherman JD, Weaver DK, Hofland ML, Sing SE, Buteler M, Lanning SP, Naruoka Y, Crutcher F, Blake NK, Martin JM, Lamb PF, Carlson GR, Talbert LE (2010) Identification of novel QTL for sawfly resistance in wheat. Crop Sci 50:73–86
Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155
Smith RCG, Heritage AD, Stapper M, Barrs HD (1986) Effect of stripe rust (Puccinia striiformis West.) and irrigation on the yield and foliage temperature of wheat. Field Crop Res 14:39–51
Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336
Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890
Sui XX, Wang MN, Chen XM (2009) Molecular mapping of a stripe rust resistance gene in spring wheat cultivar Zak. Phytopathology 99:1209–1215
Sun G, Fahima T, Korol A, Turpeinen T, Grama A, Ronin Y, Nevo E (1997) Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat Triticum dicoccoides. Theor Appl Genet 94:622–628
Wilhelm EP, Turner AS, Laurie DA (2009) Photoperiod insensitive Ppd-A1a mutations in tetraploid wheat (Triticum durum Desf.). Theor Appl Genet 118:285–294
Xue S, Zhang Z, Lin F, Kong Z, Cao Y, Li C, Yi H, Mei M, Zhu H, Wu J, Xu H, Zhao D, Tian D, Zhang C, Ma Z (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117:181–189
Yan L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen JL, Echenique V, Dubcovsky J (2004) The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science 303:1640–1644
Acknowledgments
This project was supported by the National Research Initiative Competitive Grants 2009-65300-05640 and 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. I. Lowe received valuable support through the Graduate Student Research Fellowship program of the UC Davis Department of Plant Sciences. L. Jankuloski received fellowship support from the International Atomic Energy Agency. The authors thank O. Chicaiza, Z. Abate, A. Distelfeld, D. Fu, F. Paraiso, C. Uauy, A. Wan, D. Feltus, M. Osenga, V. Talbott, and especially X. Zhang for excellent technical assistance.
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Communicated by P. Langridge.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Online Resource 1.
Detailed description, with images, of the parental phenotypes and the 11-point reaction type disease evaluation scale used in this study (PDF 1922 kb)
Online Resource 2.
Complete genetic map for the UC1110/PI610750 mapping population, including detailed notes and summary QTL information (PDF 222 kb)
Online Resource 3.
Searchable spreadsheet of the complete UC1110/PI610750 genetic map, including all genotypic and phenotypic data used in this study; also GSTR assignments of deposited lines (XLS 1.54 mb)
Online Resource 4.
Supplementary figures (S1-S6): Correlation scatterplots among replications within each year, correlation scatterplots between years, LOD plots of the sub-threshold 3D and 7AL QTL regions, QTL interaction plots (11-point reaction type), bar charts of effects of isolated QTL regions (reaction type), and bar chart of effects of 3BS and 5AL QTL regions by year (PDF 526 kb)
Online Resource 5.
Supplementary tables (S1-S3) summarizing validation and dissection data for the 3BS, 5AL, and 2BS QTL regions (PDF 740 kb)
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Lowe, I., Jankuloski, L., Chao, S. et al. Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theor Appl Genet 123, 143–157 (2011). https://doi.org/10.1007/s00122-011-1573-0
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DOI: https://doi.org/10.1007/s00122-011-1573-0