Abstract
PK23-2, a line of six-rowed barley (Hordeum vulgare L.) originating from Pakistan, has resistance to Japanese strains I and III of the barley yellow mosaic virus (BaYMV). To identify the source of resistance in this line, reciprocal crosses were made between the susceptible cultivar Daisen-gold and PK23-2. Genetic analyses in the F1 generation, F2 generation, and a doubled haploid population (DH45) derived from the F1 revealed that PK23-2 harbors one dominant and one recessive resistance genes. A linkage map was constructed using 61 lines of DH45 and 127 DNA markers; this map covered 1268.8 cM in 10 linkage groups. One QTL having a LOD score of 4.07 and explaining 26.8% of the phenotypic variance explained (PVE) for resistance to BaYMV was detected at DNA marker ABG070 on chromosome 3H. Another QTL having a LOD score of 3.53 and PVE of 27.2% was located at marker Bmag0490 on chromosome 4H. The resistance gene on chromosome 3H, here named Rym17, showed dominant inheritance, whereas the gene on chromosome 4H, here named rym18, showed recessive inheritance in F1 populations derived from crosses between several resistant lines of DH45 and Daisen-gold. The BaYMV recessive resistance genes rym1, rym3, and rym5, found in Japanese barley germplasm, were not allelic to rym18. These results revealed that PK23-2 harbors two previously unidentified resistance genes, Rym17 on 3H and rym18 on 4H; Rym17 is the first dominant BaYMV resistance gene to be identified in primary gene pool. These new genes, particularly dominant Rym17, represent a potentially valuable genetic resource against BaYMV disease.
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References
Bauer E, Weyen J, Schiemann A, Graner A, Ordon F (1997) Molecular mapping of novel resistance genes against barley mild mosaic virus (BaMMV). Theor Appl Genet 95:1263–1269
Blake TK, Kadyrzhanova D, Shepherd KW, Islam AKMR, Langridge PL, McDonald CL, Erpelding J, Larson S, Blake NK, Talbert LE (1996) STS-PCR markers appropriate for wheat–barley introgression. Theor Appl Genet 93:826–832
Chisholm ST, Mahajan SK, Whitham SA, Yamamoto ML, Carrington JC (2000) Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus. Proc Natl Acad Sci USA 97:489–494
Cosson P, Sofer L, Le QH, Léger V, Schurdi-Levraud V, Whitham SA, Yamamoto ML, Gopalan S, Gall OL, Candresse T, Carrington JC, Revers F (2010) RTM3, which controls long-distance movement of potyviruses, is a member of a new plant gene family encoding a meprin and TRAF homology domain-containing protein. Plant Physiol 154:222–232
Furusho M, Hamachi Y, Yoshida T (1990) Variety difference in crossability between Japanese two-rowed barley and Hordeum bulbosum L. Japan J Breed 40:411–417
Furusho M, Tanifuji K, Gamo T, Ahmad Z, Shahid Masood M (1997) Collaborative exploration of wheat, barley and their wild relatives in Pakistan, 1996. Annual Report on Exploration and Introduction of PGR 13:59-86
Furusho M, Kai H, Tsukazaki M (2002) The dominant resistance gene to barley yellow mosaic virus from barley germplasms collected in Pakistan. Breed Res 4(2):296
Graner A, Bauer E (1993) RFLP mapping of the ym4 virus resistance gene in barley. Theor Appl Genet 86:689–693
Graner A, Bauer E, Kellermann A, Proeseler G, Wenzel G, Ordon F (1995) RFLP analysis of resistance to the barley yellow mosaic virus complex. Agronomie 15:475–479
Graner A, Streng S, Kellermann A, Proeseler G, Schiemann A, Peterka H, Ordon F (1999) Molecular mapping of genes conferring resistance to soil-borne viruses in barley. An approach to promote understanding of host–pathogen interactions. J Plant Dis Protec 106:405–410
Iida Y, Ban T, Konishi T (1999) Linkage analysis of the rym6 resistance gene to Japanese strain II of barley yellow mosaic virus (BaYMV-II) in barley. Barley Genet Newsl 29:31–32
Kashiwazaki S, Ogawa K, Usugi T, Omura T, Tsuchizaki T (1989) Characterization of several strains of barley yellow mosaic virus. Ann Phytopath Soc Jpn 55:16–25
Konishi T, Ban T, Iida Y, Yoshimi R (1997) Genetic analysis of disease resistance to all strains of BaYMV in a Chinese barley landrace, Mokusekko 3. Theor Appl Genet 94:871–877
Le Gouis J, Devaux P, Werner K, Hariri D, Bahrman N, Béghin D, Ordon F (2004) rym15 from the Japanese cultivar Chikurin Ibaraki 1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on chromosome 6H. Theor Appl Genet 108:1521–1525
Mano Y, Sayed-Tabatabaei B, Graner A, Blake T, Takaiwa F, Oka S, Komatsuda T (1999) Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.). Theor Appl Genet 98:937–946
Ordon F, Friedt W (1993) Mode of inheritance and genetic diversity of BaMMV resistance of exotic barley germplasms carrying genes different from ‘ym4’. Theor Appl Genet 86:229–233
Ordon F, Friedt W, Scheurer K, Pellio B, Werner K, Neuhaus G, Huth W, Habekuss A, Graner A (2004) Molecular markers in breeding for virus resistance in barley. J Appl Genet 45:145–159
Ramsay L, Macaulay M, Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards K, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005
Ruge B, Linz A, Pickering R, Proeseler G, Greif P, Wehling P (2003) Mapping of Rym14 HB, a gene introgressed from Hordeum bulbosum and conferring resistance to BaYMV in barley. Theor Appl Genet 107:965–971
Ruge-Wehling B, Linz A, Habekuß A, Wehling P (2006) Mapping of Rym16 HB, the second soil-borne virus-resistance gene introgressed from Hordeum bulbosum. Theor Appl Genet 113:867–873
Sotome T, Kawada N, Kato T, Sekiwa T, Nishigawa H, Natsuaki T, Kimura K, Meoka Y, Nagamine T, Kobayashi S, Wada Y, Yoshida T (2010) The current and new strains of barley yellow mosaic virus (BaYMV) in Tochigi prefecture. Jpn J Crop Sci 79:29–36
Stein N, Perovic D, Kumlehn J, Pellio B, Stracke S, Streng S, Ordon F, Graner A (2005) The eukaryotic translation initiation factor 4E confers multiallelic recessive Bymovirus resistance in Hordeum vulgare (L.). Plant J 42:912–922
Toyama A, Kusaba T (1970) Transmission of soil-borne barley yellow mosaic virus. 2. Polymyxa graminis Led. as vector. Ann Phytopath Soc Jpn 36:223–229
Ukai Y, Yamashita A (1980) Induced mutation for resistance to barley yellow mosaic virus. Jpn J Breed 30:125–130
Ukai Y, Ohsawa R, Saito A, Hayashi T (1995) MAPL: a package of computer programs for construction of DNA polymorphism linkage map and analysis of QTL. Breed Sci 45:139–142
Usugi T, Kuwabara T, Tsuchizaki T (1984) Serological detection of barley yellow mosaic virus, wheat yellow mosaic virus and soil-borne wheat mosaic virus by ELISA. Ann Phytopath Soc Jpn 50:63–68
Werner K, Rönicke S, Gouis J, Friedt W, Ordon F (2003) Mapping of a new BaMMV-resistance gene derived from the variety ‘Taihoku A’. J Plant Dis Protec 110:304–311
Whitham SA, Anderberg RJ, Chisholm ST, Carrington JC (2000) Arabidopsis RTM2 gene is necessary for specific restriction of tobacco etch virus and encodes an unusual small heat shock-like protein. Plant Cell 12:569–582
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This work was conducted by the malting barley breeding program in Fukuoka Agricultural Research Center, and supported by a Grant-in-Aid from the Ministry of Agriculture, Forestry and Fisheries of Japan.
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Communicated by A. Graner.
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Kai, H., Takata, K., Tsukazaki, M. et al. Molecular mapping of Rym17, a dominant and rym18 a recessive barley yellow mosaic virus (BaYMV) resistance genes derived from Hordeum vulgare L.. Theor Appl Genet 124, 577–583 (2012). https://doi.org/10.1007/s00122-011-1730-5
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DOI: https://doi.org/10.1007/s00122-011-1730-5