Abstract
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This study elucidates the influence of indehiscent mutations on rapeseed silique shatter resistance. A phenotype with enlarged replum-valve joint area and altered cell dimensions in the dehiscence zone is described.
Abstract
Silique shattering is a major factor reducing the yield stability of oilseed rape (Brassica napus). Attempts to improve shatter resistance often include the use of mutations in target genes identified from Arabidopsis (Arabidopsis thaliana). A variety of phenotyping methods assessing the level of shatter resistance were previously described. However, a comparative and comprehensive evaluation of the methods has not yet been undertaken. We verified the increase of shatter resistance in indehiscent double knock-down mutants obtained by TILLING with a systematic approach comparing three independent phenotyping methods. A positive correlation of silique length and shatter resistance was observed and accounted for in the analyses. Microscopic studies ruled out the influence of different lignification patterns. Instead, we propose a model to explain increased shattering resistance of indehiscent rapeseed mutants by altered cell shapes and sizes within the contact surfaces of replum and valves.
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Acknowledgements
This study was financed by the Stiftung Schleswig-Holsteinische Landschaft under Grant no. 2013/69. We thank Monika Bruisch, Hilke Jensen, Lara Wostupatsch, and Niloufar Nezaratizadeh for technical assistance; Mario Hasler for support on statistical analyses; Maria Mulisch and Cay Kruse for assistance with light microscopy of cryosections; the Institute of Clinical Molecular Biology in Kiel for Sanger sequencing; the breeding company Norddeutsche Pflanzenzucht Hans-Georg Lembke for collaboration regarding the EMS mutant population.
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Braatz, J., Harloff, HJ., Emrani, N. et al. The effect of INDEHISCENT point mutations on silique shatter resistance in oilseed rape (Brassica napus). Theor Appl Genet 131, 959–971 (2018). https://doi.org/10.1007/s00122-018-3051-4
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DOI: https://doi.org/10.1007/s00122-018-3051-4