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Mapping Solanum chacoense mediated Colorado potato beetle (Leptinotarsa decemlineata) resistance in a self-compatible F2 diploid population

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A major QTL on chromosome 2 associated with leptine biosynthesis and Colorado potato beetle resistance was identified in a diploid S. chacoense F2 population using linkage mapping and bulk-segregant analysis.

Abstract

We examined the genetic features underlying leptine glycoalkaloid mediated Colorado potato beetle (Leptinotarsa decemlineata) host plant resistance in a diploid F2 mapping population of 233 individuals derived from Solanum chacoense lines USDA8380-1 and M6. The presence of foliar leptine glycoalkaloids in this population segregated as a single dominant gene and displayed continuous distribution of accumulated quantity in those individuals producing the compound. Using biparental linkage mapping, a major overlapping QTL region with partial dominance effects was identified on chromosome 2 explaining 49.3% and 34.1% of the variance in Colorado potato beetle field resistance and leptine accumulation, respectively. Association of this putative resistance region on chromosome 2 was further studied in an expanded F2 population in a subsequent field season. Loci significantly associated with leptine synthesis colocalized to chromosome 2. Significant correlation between increased leptine content and decreased Colorado potato beetle defoliation suggests a single QTL on chromosome 2. Additionally, a minor QTL with overdominance effects explaining 6.2% associated with Colorado potato beetle resistance donated by susceptible parent M6 was identified on chromosome 7. Bulk segregant whole genome sequencing of the same F2 population detected QTL associated with Colorado potato beetle resistance on chromosomes 2, 4, 6, 7, and 12. Weighted gene co-expression network analysis of parental lines and resistant and susceptible F2 individuals identified a tetratricopeptide repeat containing protein with a putative regulatory function and a previously uncharacterized acetyltransferase within the QTL region on chromosome 2, possibly under the control of a regulatory Tap46 subunit within the minor QTL on chromosome 12.

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Acknowledgements

The authors are grateful to Robin Buell and Gina Pham for advice on sequencing and bioinformatic analyses. We would also like to thank Anthony Schilmiller, Assistant Core Manager of the Michigan State University Mass Spectrometry and Metabolomics Core facility, for guidance on methods and Grant Billings for his technical assistance conducting Colorado potato beetle bioassays, extracting metabolite and DNA samples, and providing plant maintenance in the greenhouse.

Funding

This project was supported by Michigan State University AgBioResearch Project GREEEN (Generating Research and Extension to meet Economic and Environmental Needs) (Grant No. GR17-014) funding.

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Authors and Affiliations

  1. Department of Plant, Soil and Microbial Sciences, 1130 C Molecular Plant Sciences, Michigan State University, 1066 Bogue Street, East Lansing, MI, 48824, USA

    Natalie Kaiser, Joseph Coombs & David Douches

  2. Department of Entomology, Michigan State University, East Lansing, MI, 48824, USA

    Christina DiFonzo

  3. International Potato Center (CIP) CGIAR, Lima, Peru

    Norma Constanza Manrique-Carpintero

Authors
  1. Natalie Kaiser
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  2. Norma Constanza Manrique-Carpintero
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  3. Christina DiFonzo
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  4. Joseph Coombs
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  5. David Douches
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Contributions

NRK and DD conceived of this study and contributed to funding acquisition. NRK, CD, DD, NCMC, and JC designed the research. NRK executed the experiments. NRK and NCMC defined linkage mapping protocols. NRK, CD. and JC defined insect assay experimental design and protocols. NRK defined genomic analysis pipelines. NRK analyzed the data with input from all authors. NRK and NCMC wrote the manuscript with contributions from all coauthors.

Corresponding author

Correspondence to Natalie Kaiser.

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The authors declare that they have no conflict of interest.

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Communicated by Jeffrey Endelman.

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Kaiser, N., Manrique-Carpintero, N.C., DiFonzo, C. et al. Mapping Solanum chacoense mediated Colorado potato beetle (Leptinotarsa decemlineata) resistance in a self-compatible F2 diploid population. Theor Appl Genet 133, 2583–2603 (2020). https://doi.org/10.1007/s00122-020-03619-8

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  • DOI: https://doi.org/10.1007/s00122-020-03619-8

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