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In Vitro Determination of 6PGD Enzyme Activity Purified from Lake Van Fish (Chalcalburnus tarichii Pallas, 1811) Liver Exposed to Pesticides

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Abstract

In the present study, the effect of methidathion, cypermethrin, and deltamethrin pesticides on Lake Van fish (Chalcalburnus tarichii Pallas, 1811) liver 6-phosphogluconate dehydrogenase enzyme activity was investigated due to the fact that these pesticides are extensively used to improve agricultural productivity in the Van region. 2′,5′-ADP Sepharose 4B affinity chromatography was used to purify 6-phosphogluconate dehydrogenase enzyme from fish liver and SDS-PAGE technique was used to control the purity of this enzyme. The in vitro effect of methidathion, cypermethrin, and deltamethrin pesticides on the enzyme activity was investigated. The enzyme was purified 1,050-fold with specific activity of 27.04 EU/mg protein. Moreover, Ki constants of methidathion, cypermethrin, and deltamethrin were to be 3.294 ± 0.215, 0.718 ± 0.095, and 0.084 ± 0.009 mM respectively. The IC50 value were estimated as 9.95 × 10−5 ± 0.1844 × 10−5 mM for methidathion, 1.01 × 10−4 ± 0.01413 × 10−4 mM for cypermethrin, and 4.43 × 10−6 ± 0.05653 × 10−6 mM for deltamethrin. In conclusion, deltamethrin inhibits the enzyme activity more than methidathion and cypermethrin.

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Acknowledgments

Authors would like to thank the administrative units of the scientific research projects of Van Yuzuncu Yil University for the financial support.

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Authors and Affiliations

  1. Department of Chemistry, Yuzuncu Yıl University, 65080, Van, Turkey

    Muhammet Guler, M. Riza Kivanc, Vedat Turkoglu & Zehra Basi

  2. Chemical Engineering Department, Selcuk University, 42031, Konya, Turkey

    Hilal Kivrak

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  1. Muhammet Guler
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  2. M. Riza Kivanc
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  3. Vedat Turkoglu
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  4. Zehra Basi
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  5. Hilal Kivrak
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Correspondence to Muhammet Guler.

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Guler, M., Kivanc, M.R., Turkoglu, V. et al. In Vitro Determination of 6PGD Enzyme Activity Purified from Lake Van Fish (Chalcalburnus tarichii Pallas, 1811) Liver Exposed to Pesticides. Bull Environ Contam Toxicol 91, 560–564 (2013). https://doi.org/10.1007/s00128-013-1096-2

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  • DOI: https://doi.org/10.1007/s00128-013-1096-2

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