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Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery

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Abstract

Introduction and hypothesis

Mesh-augmented reconstructive surgery for pelvic organ prolapse (POP) does not meet clinical expectations. A tissue-engineered fascia equivalent needs to be developed.

Methods

Human vaginal fibroblasts (HVFs) from 10 patients were characterized in vitro. Eligible HVFs and a biodegradable scaffold were used to fabricate a fascia equivalent, which was then transplanted in vivo.

Results

The cultured HVFs were divided into high (n = 6) or low (n = 4) collagen I/III ratio groups. Cells of the high-ratio group exhibited significantly higher proliferation potential than those of the low-ratio group (P < 0.05). A fascia equivalent was made with HVFs of the high-ratio group. In the subsequent animal study, a well-organized neo-fascia formation containing HVFs could be traced up to 12 weeks after transplantation.

Conclusions

Our results suggest that a tissue-engineered fascia could be developed from HVFs in vitro and in vivo, which might be an effective treatment for POP in the future.

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Abbreviations

POP:

Pelvic organ prolapse

SUI:

Stress urinary incontinence

HVFs:

Human vaginal fibroblasts

PBS:

Phosphate-buffered saline

DMEM:

Dulbecco’s modified Eagle medium

FBS:

Fetal bovine serum

FITC:

Fluorescein isothiocyanate

DAPI:

4,6-Diamidino-2-phenylindole dihydrochloride

HRP:

Horseradish peroxidase

ECL:

Enhanced chemiluminescence

PLGA:

Poly-dl-lactico-glycolic acid

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Acknowledgments

We thank MY Lee and YF Lin for their technical assistance. This study was supported by Taichung Veterans General Hospital and Tunghai University (TCVGH-T) grant no. 947808, 957802, and 967803.

Conflicts of interest

None.

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, Taichung, Taiwan

    Man-Jung Hung, Chia-Nung Hung & Esther Shih-Chu Ho

  2. Department of Pathology, Taichung Veterans General Hospital, Taichung, Taiwan

    Mei-Chin Wen

  3. Department of Obstetrics and Gynecology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung, Taiwan

    Man-Jung Hung, Esther Shih-Chu Ho & Gin-Den Chen

  4. Department of Life Science, Tunghai University, No. 181, Section 3, Taichung Port Road, Taichung, Taiwan

    Man-Jung Hung, Chia-Nung Hung & Vivian Cheng Yang

  5. Department of Nutrition, Providence University, Taichung, Taiwan

    Man-Jung Hung

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  1. Man-Jung Hung
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  2. Mei-Chin Wen
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  3. Chia-Nung Hung
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  4. Esther Shih-Chu Ho
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  5. Gin-Den Chen
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  6. Vivian Cheng Yang
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Correspondence to Vivian Cheng Yang.

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Hung, MJ., Wen, MC., Hung, CN. et al. Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery. Int Urogynecol J 21, 1085–1093 (2010). https://doi.org/10.1007/s00192-010-1168-3

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  • DOI: https://doi.org/10.1007/s00192-010-1168-3

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