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Isolation and characterization of rat intestinal bacteria involved in biotransformation of (−)-epigallocatechin

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Abstract

Two intestinal bacterial strains MT4s-5 and MT42 involved in the degradation of (−)-epigallocatechin (EGC) were isolated from rat feces. Strain MT4s-5 was tentatively identified as Adlercreutzia equolifaciens. This strain converted EGC into not only 1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol (1), but also 1-(3, 5-dihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol (2), and 4′-dehydroxylated EGC (7). Type strain (JCM 9979) of Eggerthella lenta was also found to convert EGC into 1. Strain MT42 was identified as Flavonifractor plautii and converted 1 into 4-hydroxy-5-(3, 4, 5-trihydroxyphenyl)valeric acid (3) and 5-(3, 4, 5-trihydroxyphenyl)-γ-valerolactone (4) simultaneously. Strain MT42 also converted 2 into 4-hydroxy-5-(3, 5-dihydroxyphenyl)valeric acid (5), and 5-(3, 5-dihydroxyphenyl)-γ-valerolactone (6). Furthermore, F. plautii strains ATCC 29863 and ATCC 49531 were found to catalyze the same reactions as strain MT42. Interestingly, formation of 2 from EGC by strain MT4s-5 occurred rapidly in the presence of hydrogen supplied by syntrophic bacteria. Strain JCM 9979 also formed 2 in the presence of the hydrogen or formate. Strain MT4s-5 converted 1, 3, and 4 to 2, 5, and 6, respectively, and the conversion was stimulated by hydrogen, whereas strain JCM 9979 could catalyze the conversion only in the presence of hydrogen or formate. On the basis of the above results together with previous reports, the principal metabolic pathway of EGC and EGCg by catechin-degrading bacteria in gut tract is proposed.

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Acknowledgments

We thank Matsumoto K. in our laboratories for analysis of 16S rRNA gene sequences of bacterial isolates. And we acknowledge the assistance of Andrea K. Suzuki (Mitsui Norin Co. Ltd.) in the review of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Food Research Laboratories, Mitsui Norin. Co., Ltd., 223-1 Miyabara, Fujieda-shi, Shizuoka, 426-0133, Japan

    Akiko Takagaki, Yuko Kato & Fumio Nanjo

Authors
  1. Akiko Takagaki
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  2. Yuko Kato
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  3. Fumio Nanjo
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Corresponding author

Correspondence to Akiko Takagaki.

Additional information

Communicated by Erko Stackebrandt.

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Takagaki, A., Kato, Y. & Nanjo, F. Isolation and characterization of rat intestinal bacteria involved in biotransformation of (−)-epigallocatechin. Arch Microbiol 196, 681–695 (2014). https://doi.org/10.1007/s00203-014-1006-y

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  • DOI: https://doi.org/10.1007/s00203-014-1006-y

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