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Harenicola maris gen. nov., sp. nov. isolated from the Sea of Japan shallow sediments

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Abstract

A Gram-negative, non-motile bacterium КMM 3653T was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КMM 3653T was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2–94.7%) and Nioella (95.1–94.5%), respectively. According to the phylogenomic tree based on 400 conserved protein sequences, strain КMM 3653T was placed in the cluster comprising Vannielia litorea, Nioella nitratireducens, Litoreibacter albidus and Pseudoruegeria aquimaris as a separate lineage adjacent to V. litorea KCTC 32083T. The average nucleotide identity values between strain КMM 3653T and V. litorea KCTC 32083T, N. nitratireducens KCTC 32417T, L. albidus KMM 3851T, and P. aquimaris CECT 7680T were 71.1, 70.3, 69.6, and 71.0%, respectively. Strain КMM 3653T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid followed by C16:0. The polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified aminolipids, and five unidentified lipids. The DNA G+C content of 61.8% was calculated from the genome sequence. Based on the phylogenetic evidence and distinctive phenotypic characteristics, we proposed strain KMM 3653T (= KCTC 82575T) to be classified as a novel genus and species Harenicola maris gen. nov., sp. nov.

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Data and materials availability

The DDBJ/ENA/GenBank accession number for the 16S rRNA gene sequence and the complete genome sequence of the strain KММ 3653T are LC595119 and JADQAZ000000000, respectively. The version described in this paper is version JADQAZ010000000.

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia

    Lyudmila A. Romanenko, Valeriya V. Kurilenko, Nadezhda Y. Chernysheva & Marina P. Isaeva

  2. Innovative Technology Center, Far Eastern Federal University, 8 Suhanova St, Vladivostok, 690950, Russia

    Liudmila A. Tekutyeva

  3. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia

    Peter V. Velansky & Vassilii I. Svetashev

Authors
  1. Lyudmila A. Romanenko
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  2. Valeriya V. Kurilenko
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  3. Nadezhda Y. Chernysheva
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  6. Vassilii I. Svetashev
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Correspondence to Lyudmila A. Romanenko.

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Communicated by Erko Stackebrandt.

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Romanenko, L.A., Kurilenko, V.V., Chernysheva, N.Y. et al. Harenicola maris gen. nov., sp. nov. isolated from the Sea of Japan shallow sediments. Arch Microbiol 203, 3973–3979 (2021). https://doi.org/10.1007/s00203-021-02360-0

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