Abstract
A novel Gram-staining negative, strictly aerobic, rod-shaped, and non-motile bacterium, designated strain 9Alg 56T, was isolated from the red alga Tichocarpus crinitus. The phylogenetic analysis based on 16S rRNA gene sequences placed the novel strain within the family Rhodobacteraceae, the order Rhodobacterales, the class Alphaproteobacteria, the phylum Pseudomonadota. The nearest neighbors of the new strain were Pontivivens insulae KCTC 42458T, Oceanibium sediminis KCTC 62076T, Halovulum dunhuangense YYQ-30T and Monaibacterium marinum C7T with 16S rRNA gene sequence similarity of 94.7, 94.4%, 93.1 and 92.7%, respectively. The AAI/ANI/dDDH values between 9Alg 56T and the five species of the closest genera (Pontivivens, Oceanibium, Halovulum, Monaibacterium, and ‘Oceanomicrobium’) were 58.63–63.91%/ 75.91–77.37%/ 19.3–20.4%. The prevalent fatty acids of strain 9Alg 56T were C18:1 ω7c, C18:0 and C14:0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, phosphatidylcholine, and two unidentified lipids. The DNA G+C content of strain 9Alg 56T was 61.5 mol%. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel genus and species, for which the name Algicella marina gen. nov., sp. nov. is proposed. The type strain is 9Alg 56T (= KCTC 72005T = KMM 6775T).
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This research was funded by a grant from the Ministry of Science and Higher Education, Russian Federation 15.BRK.21.0004 (Contract No. 075-15-2021-1052).
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O.N., S-G.K. and M.I. planned the research and wrote the main manuscript text. O.N., S-G.K., A.K., and N.Z. carried out experiments. O.N., S-G.K., N.O., N.Z. and M.I. analysed the data and prepared figures. O.N. and M.I. supervised the research. All authors proofread the manuscript.
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The whole genome sequence and 16S rRNA of Algicella marina gen. nov., sp. nov. strain 9Alg 56T have been deposited into GenBank/EMBL/DDBJ under accession numbers CP046620 and MN813959, respectively.
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Nedashkovskaya, O.I., Otstavnykh, N.Y., Kim, SG. et al. Algicella marina gen. nov., sp. nov., a novel marine bacterium isolated from a Pacific red alga. Arch Microbiol 204, 487 (2022). https://doi.org/10.1007/s00203-022-03091-6
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DOI: https://doi.org/10.1007/s00203-022-03091-6