Abstract
Concerning the potential application of the optically active isomer (R,R)-2,3-butanediol, and its production by a non-pathogenic bacterium Paenibacillus polymyxa ATCC 842, the present study evaluated the use of a commercial crude yeast extract Nucel®, as an organic nitrogen and vitamin source, at different medium composition and two airflows (0.2 or 0.5 vvm). The medium formulated (M4) with crude yeast extract carried out with the airflow of 0.2 vvm (experiment R6) allowed for a reduction in the cultivation time and kept the dissolved oxygen values at low levels until the total glucose consumption. Thus, the experiment R6 led to a fermentation yield of 41% superior when compared to the standard medium (experiment R1), which was conducted at airflow of 0.5 vvm. The maximum specific growth rate at R6 (0.42 h−1) was lower than R1 (0.60 h−1), however, the final cell concentration was not affected. Moreover, this condition (medium formulated—M4 and low airflow—0.2 vvm) was a great alternative to produce (R,R)-2,3-BD at fed-batch mode, resulting in 30 g.L−1 of the isomer at 24 h of cultivation, representing the main product in the broth (77%) and with a fermentation yield of 80%. These results showed that both medium composition and oxygen supply have an important role to produce 2,3-BD by P. polymyxa.
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Data generated during the current study are available from the corresponding author on reasonable request.
References
Adlakha N, Yazdani SS (2015) Efficient production of (R, R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008. J Ind Microbiol Biotechnol 42:21–28. https://doi.org/10.1007/s10295-014-1542-0
Adlakha N, Pfau T, Ebenhöh O, Yazdani SS (2015) Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008. Biotechnol Biofuels 8:1–10. https://doi.org/10.1186/s13068-015-0338-4
Berbert-Molina MA, Sato S, Silveira MM (2001) Ammonium phosphate as a sole nutritional supplement for the fermentative production of 2,3-butanediol from sugar cane juice. Z Naturforschung Sect C J Biosci. https://doi.org/10.1515/znc-2001-9-1017
Białkowska AM (2016) Strategies for efficient and economical 2,3-butanediol production: new trends in this field. World J Microbiol Biotechnol. https://doi.org/10.1007/s11274-016-2161-x
Celińska E, Grajek W (2009) Biotechnological production of 2,3-butanediol-Current state and prospects. Biotechnol Adv 27:715–725. https://doi.org/10.1016/j.biotechadv.2009.05.002
Dai JJ, Cheng JS, Liang YQ et al (2014) Regulation of extracellular oxidoreduction potential enhanced (R, R)-2,3-butanediol production by Paenibacillus polymyxa CJX518. Bioresour Technol 167:433–440. https://doi.org/10.1016/j.biortech.2014.06.044
Dai J, Wang Z, Long Z (2019) High production of optically pure (3R)-acetoin by a newly isolated marine strain of Bacillus subtilis CGMCC 13141. Bioprocess Biosyst Eng 42:475–483. https://doi.org/10.1007/s00449-018-2051-8
de Mas C, Jansen NB, Tsao GT (1988) Production of optically active 2,3-butanediol by Bacillus polymyxa. Biotechnol Bioeng 31:366–377
de Souza BC, Bossardi FF, Furlan GR et al (2021) Validated high-performance liquid chromatographic (HPLC) method for the simultaneous quantification of 2,3-butanediol, glycerol, acetoin, ethanol, and phosphate in microbial cultivations. Anal Lett. https://doi.org/10.1080/00032719.2020.1869754
Gao J, Jiang F, Cao C et al (2019) Effects of amino acids on the fermentation of inulin or glucose to produce R, R-2,3-butanediol using Paenibacillus polymyxa ZJ-9. Lett Appl Microbiol 69:424–430. https://doi.org/10.1111/lam.13234
Hakizimana O, Matabaro E, Lee BH (2020) The current strategies and parameters for the enhanced microbial production of 2,3-butanediol. Biotechnol Rep 25:e00397. https://doi.org/10.1016/j.btre.2019.e00397
Häßler T, Schieder D, Pfaller R et al (2012) Enhanced fed-batch fermentation of 2,3-butanediol by Paenibacillus polymyxa DSM 365. Bioresour Technol 124:237–244. https://doi.org/10.1016/j.biortech.2012.08.047
Hazeena SH, Sindhu R, Pandey A, Binod P (2020) Lignocellulosic bio-refinery approach for microbial 2,3-butanediol production. Bioresour Technol. https://doi.org/10.1016/j.biortech.2020.122873
He Y, Chen F, Sun M et al (2018) Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis. Molecules 23:691. https://doi.org/10.3390/molecules23030691
Jansen NB, Flickinger MC, Tsao GT (1984) Production of 2,3-butanediol fromD-xylose byKlebsiella oxytoca ATCC 8724. Biotechnol Bioeng 26:362–369. https://doi.org/10.1002/bit.260260411
Ji XJ, Huang H, Ouyang PK (2011) Microbial 2,3-butanediol production: A state-of-the-art review. Biotechnol Adv 29:351–364. https://doi.org/10.1016/j.biotechadv.2011.01.007
Ma C, Wang A, Qin J et al (2009) Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM. Appl Microbiol Biotechnol 82:49–57. https://doi.org/10.1007/s00253-008-1732-7
Ma K, He M, You H et al (2018) Improvement of (R, R)-2,3-butanediol production from corn stover hydrolysate by cell recycling continuous fermentation. Chem Eng J 332:361–369. https://doi.org/10.1016/j.cej.2017.09.097
Maina S, Mallouchos A, Nychas GJE et al (2019) Bioprocess development for (2R,3R)-butanediol and acetoin production using very high polarity cane sugar and sugarcane molasses by a Bacillus amyloliquefaciens strain. J Chem Technol Biotechnol 94:2167–2177. https://doi.org/10.1002/jctb.5997
Marwoto B, Nakashimada Y, Kakizono T, Nishio N (2002) Enhancement of (R, R)-2,3-butanediol production from xylose by Paenibacillus polymyxa at elevated temperatures. Biotechnol Lett 24(2):109–114
Mitsui R, Yamada R, Matsumoto T, Ogino H (2022) Bioengineering for the industrial production of 2,3-butanediol by the yeast Saccharomyces cerevisiae. World J Microbiol Biotechno. https://doi.org/10.1007/s11274-021-03224-x
Nakashimada Y, Kanai Y, Nishio N (1998) Optimization of dilution rate, pH and oxygen supply on optical purity of 2,3-butanediol produced by Paenibacillus polymyxa in chemostat culture. Biotechnol Lett 20:1133–1138
Okonkwo C, Ujor V, Ezeji T (2017a) Investigation of relationship between 2,3-butanediol toxicity and production during growth of Paenibacillus polymyxa. N Biotechnol 34:23–31. https://doi.org/10.1016/j.nbt.2016.10.006
Okonkwo C, Ujor V, Mishra P, Ezeji T (2017b) Process development for enhanced 2,3-butanediol production by Paenibacillus polymyxa DSM 365. Fermentation 3:18. https://doi.org/10.3390/fermentation3020018
Parate RD, Rode C, v., Dharne MS, (2018) 2,3-Butanediol production from biodiesel derived glycerol. Curr Environ Eng 5:4–12. https://doi.org/10.2174/2212717805666180112162517
Petrov K, Petrova P (2010) Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations. Appl Microbiol Biotechnol 87:943–949. https://doi.org/10.1007/s00253-010-2545-z
Pirt SJ, Callow DS (1958) Exocellular product formation by microorganisms in continuous culture. I. production of 2: 3-butanediol by aerobacter aerogenes in a single stage process. J Appl Bacteriol 21:188–205
Priya A, Dureja P, Talukdar P et al (2016) Microbial production of 2,3-butanediol through a two-stage pH and agitation strategy in 150l bioreactor. Biochem Eng J 105:159–167. https://doi.org/10.1016/j.bej.2015.09.016
Qin J, Xiao Z, Ma C et al (2006) Production of 2,3-butanediol by Klebsiella pneumoniae using glucose and ammonium phosphate. Chin J Chem Eng. https://doi.org/10.1016/S1004-9541(06)60050-5
Silveira MM, Berbert-Molina M, Prata AMR, Schmidell W (1998) Production of 2,3-butanediol from sucrose by Klebsiella pneumoniae NRRL B199 in batch and fed-batch reactors. Braz Arch Biol Technol 41:329–334. https://doi.org/10.1590/S1516-89131998000300009
Song CW, Park JM, Chung SC et al (2019) Microbial production of 2,3-butanediol for industrial applications. J Ind Microbiol Biotechnol 46:1583–1601. https://doi.org/10.1007/s10295-019-02231-0
Syu MJ (2001) Biological production of 2,3-butanediol. Appl Microbiol Biotechnol 55:10–18. https://doi.org/10.1007/s002530000486
Tian Y, Fan Y, Liu J et al (2016) Effect of nitrogen, carbon sources and agitation speed on acetoin production of Bacillus subtilis SF4-3. Electron J Biotechnol 19:41–49. https://doi.org/10.1016/j.ejbt.2015.11.005
Tinôco D, de Castro AM, Seldin L, Freire DMG (2021a) Production of (2R,3R)-butanediol by Paenibacillus polymyxa PM 3605 from crude glycerol supplemented with sugarcane molasses. Process Biochem. https://doi.org/10.1016/j.procbio.2021.03.030
Tinôco D, Pateraki C, Koutinas AA, Freire DMG (2021) Bioprocess development for 2,3-butanediol production by Paenibacillus Strains. ChemBioEng Rev. https://doi.org/10.1002/cben.202000022
Walter R, Morri JG, Kell DB (1987) The roles of osmotic stress and water activity in the inhibition of the growth, glycolysis and glucose phosphotransferase system of Clostridium pasteurianum. Microbiology 133(2):259–66
Whitman WB (2015) Bergey’s Manual of Systematics of Archaea and Bacteria, Bergey’s M
Xie N-Z, Chen X-R, Wang Q-Y et al (2017) Microbial routes to (2R,3R)-2,3-butanediol: recent advances and future prospects. Curr Top Med Chem 17:2433–2439. https://doi.org/10.2174/1568026617666170504101646
Yu B, Sun J, Bommareddy RR et al (2011) Novel (2R,3R)-2,3-butanediol dehydrogenase from potential industrial strain Paenibacillus polymyxa ATCC 12321. Appl Environ Microbiol 77:4230–4233. https://doi.org/10.1128/AEM.02998-10
Yu D, Hair JO, Poe N et al (2022) Conversion of food waste into 2,3-Butanediol via thermophilic fermentation: effects of carbohydrate content and nutrient supplementation. Food 11(2):169
Acknowledgements
The authors are grateful to Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Brazil) and the Universidade de Caxias do Sul (UCS) for the financial support to this work.
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ABF designed the experiments, analyzed the data, and drafted the manuscript. BCS contributed analytical tools. CR contributed with the investigation. SC analyzed the data and guided the study. MMS, EM and AJPD designed and guided the study, and edited the manuscript. All authors read and approved the final manuscript.
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Folle, A.B., de Souza, B.C., Reginatto, C. et al. Medium composition and aeration to high (R,R)-2,3-butanediol and acetoin production by Paenibacillus polymyxa in fed-batch mode. Arch Microbiol 205, 171 (2023). https://doi.org/10.1007/s00203-023-03521-z
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DOI: https://doi.org/10.1007/s00203-023-03521-z