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Chemiluminescence determination of melatonin and some of its derivatives using potassium permanganate and formaldehyde system

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Abstract.

It was found that melatonin and its derivatives, such as N-acetyl- 5-methoxytryptamine (MT), N-acetyl-5-hydroxytryptamine (NAS), 5-Methoxytrypt- amine (5-MT), 5-Methoxyindolyl acetic acid (5-MIAA) and N-acetyl-5-methoxy- 6-hydroxytryptamine (6-HMT) would give chemiluminescence in the acidic potassium permanganate solution, and formaldehyde would enhance this chemiluminescent reaction greatly. The optimum conditions for this chemiluminescent reaction were studied in detail by a flow injection system. A new simple rapid method has been developed under the optimum conditions for determination of melatonin. This method has the advantages of high sensitivity, wide range of linear response and low detection limit. On the basis of investigation of chemiluminescent, fluorescent and UV spectra of melatonin in acidic solution containing potassium permanganate and formaldehyde, a possible mechanism of this reaction was proposed.

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Acknowledgements.

This project was financially supported by the National Nature Sciences Funding of China (grant No. 20175005) and the Sciences Foundation of the State Education Department of China. Dr Z. F. Zhao thanks to the Monash University Malaysia Research Grants 2000.

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Authors and Affiliations

  1. Department of Chemistry, Fuzhou University, 350002, Fuzhou, Fujian, China

    Guo Nan Chen, Fu Xin Huang & Xiao Ping Wu

  2. School of Engineering and Science, Monash University Malaysia, Bandar Sunway, 46150, Petaling Java, Selangor, Malaysia

    Zheng Feng Zhao

  3. Analytical and Testing Center, Fuzhou University, 350002, Fuzhou, Fujian , China

    Jian Ping Duan

Authors
  1. Guo Nan Chen
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  2. Fu Xin Huang
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  3. Xiao Ping Wu
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Correspondence to Guo Nan Chen.

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Chen, G.N., Huang, F.X., Wu, X.P. et al. Chemiluminescence determination of melatonin and some of its derivatives using potassium permanganate and formaldehyde system. Anal Bioanal Chem 376, 873–878 (2003). https://doi.org/10.1007/s00216-003-1993-z

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  • DOI: https://doi.org/10.1007/s00216-003-1993-z

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