Abstract.
A simple capillary zone electrophoresis method was developed for the quantitative enantiomeric analysis of piperazine antihistamines with teratogenic suspicion in animals. Enantioseparation of chlorcyclizine, hydroxyzine, and meclizine was performed in glycine buffer (0.6 mol L-1; pH 3.00) with sulfated β-cyclodextrin (5 mg mL-1) as a chiral selector; and the separated drugs were monitored by ultra-violet detector. The lower quantitation of the individual enantiomer is attainable at 10 µmol L-1, using an achiral piperazine drug (cyclizine) as internal standard. The method is simple and rapid with a short run time (<5 min) for the analysis of chlorcyclizine, hydroxyzine or meclizine enantiomers.
Similar content being viewed by others
References
Hardmann J G, Limbird L E, Molinoff P B, Ruddon R W, Gilmann A G (eds) (1996) Goodman & Gilman's the pharmacological basis of therapeutics. McGraw-Hill, New York
King C T G, Weaver S A, Narrod S A (1965) J Pharmacol Exp Ther 147: 391-398.
Delgado J N, Remers W A (eds) (1998) Textbook of organic medicinal and pharmaceutical chemistry. Lippincott-Raven, New York
Wang F, Khaledi M G (1996) Anal Chem 68:3460–3467
Wang F, Khaledi M G (1999) J Chromatogr B 731:187–197
D'Hulst A, Verbeke N (1996) J Chromatogr A 735:283–293
Okamoto Y, Aburatani R, Hatano K, Hatada K (1998) J Liq Chromatogr 11:2147–2163
Mao Y, Carr P W (2001) Anal Chem 73:4478–4485
Newton D W, Murray W J, Lovell N W (1982) J Pharm Sci 71:1363–1366
Niesink R J M, de Vries J, Hollinger M A (eds) (1996) Toxicology: principles and applications. CRC, New York
Acknowledgement.
The author H. L. Wu is grateful to the National Science Council (Taiwan) for financial support of this work (NSC 90–2745-P037–001 and NSC 90–2320-B037–049).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ho, Y.H., Wu, H.L., Wu, S.M. et al. Quantitative enantiomeric analysis of chlorcyclizine, hydroxyzine, and meclizine by capillary electrophoresis. Anal Bioanal Chem 376, 859–863 (2003). https://doi.org/10.1007/s00216-003-2015-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-003-2015-x