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Quantitative enantiomeric analysis of chlorcyclizine, hydroxyzine, and meclizine by capillary electrophoresis

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Abstract.

A simple capillary zone electrophoresis method was developed for the quantitative enantiomeric analysis of piperazine antihistamines with teratogenic suspicion in animals. Enantioseparation of chlorcyclizine, hydroxyzine, and meclizine was performed in glycine buffer (0.6 mol L-1; pH 3.00) with sulfated β-cyclodextrin (5 mg mL-1) as a chiral selector; and the separated drugs were monitored by ultra-violet detector. The lower quantitation of the individual enantiomer is attainable at 10 µmol L-1, using an achiral piperazine drug (cyclizine) as internal standard. The method is simple and rapid with a short run time (<5 min) for the analysis of chlorcyclizine, hydroxyzine or meclizine enantiomers.

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Acknowledgement.

The author H. L. Wu is grateful to the National Science Council (Taiwan) for financial support of this work (NSC 90–2745-P037–001 and NSC 90–2320-B037–049).

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Correspondence to Hsin- Lung Wu.

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Ho, Y.H., Wu, H.L., Wu, S.M. et al. Quantitative enantiomeric analysis of chlorcyclizine, hydroxyzine, and meclizine by capillary electrophoresis. Anal Bioanal Chem 376, 859–863 (2003). https://doi.org/10.1007/s00216-003-2015-x

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  • DOI: https://doi.org/10.1007/s00216-003-2015-x

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