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The pH-dependence of preferential solvation as studied by intermolecular homo- and heteronuclear NOE measurements of adenosine in water–trifluoroethanol mixtures

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Abstract

A study of the state of solvation of the adenine ring in adenosine and adenosine 5′-monophosphate disodium salt in water and in a (7.5:2.5) water–TFE mixture has been carried out by measurement of homo- and heteronuclear intermolecular NOE enhancements between water or TFE and the aromatic protons of these compounds. The results give evidence of site specificity in solute–solvent interaction for both solvent systems and preferential solvation of the solute by TFE in the water–TFE mixture. Significant pH dependence of these interactions has been discovered.

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Acknowledgment

This work was funded by the Deutsche Forschungsgemeinschaft (SFB-610 “Variation in Protein Conformation: Cellbiological and Pathological Relevance”, Project A2) and the Fonds der Chemischen Industrie. M.A. is grateful to Vicerrectorado de Investigación of the Universidad de Sevilla for a short-stay grant.

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  1. Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, 04103, Leipzig, Germany

    Manuel Angulo & Stefan Berger

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  1. Manuel Angulo
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  2. Stefan Berger
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Correspondence to Stefan Berger.

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Angulo, M., Berger, S. The pH-dependence of preferential solvation as studied by intermolecular homo- and heteronuclear NOE measurements of adenosine in water–trifluoroethanol mixtures. Anal Bioanal Chem 378, 1555–1560 (2004). https://doi.org/10.1007/s00216-003-2308-0

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  • DOI: https://doi.org/10.1007/s00216-003-2308-0

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