Abstract
Body burdens from exposures to pesticides may be estimated from urinary analyses of pesticide parent/metabolite concentrations. Pesticide applicators and others are often exposed to numerous unrelated pesticides, either sequentially or simultaneously. Classically, body burdens of pesticides are analyzed using chemical/instrumental analysis (CIM) or enzyme immunoassays (EIAs). Both of these technologies can usually be used to quantitate one analyte (or closely related groups of analytes) per analysis. Alternatively, multiple analytes can be measured simultaneously using a multiplexed fluorescence covalent microbead immunoassay (FCMIA). We developed a multiplexed FCMIA to simultaneously measure glyphosate (Gly), atrazine (Atz), and metolachlor mercapturate (MM) in water and urine. The assay had least detectable doses (LDDs) in water/diluted urine of 0.11/0.09 ng/ml (Gly, water/urine LDD), 0.10/0.07 ng/ml (Atz), and 0.09/0.03 ng/ml (MM). The sensitivity for the measurement of Gly was enhanced by derivatization. All assays gave linear responses from the LDDs for each respective pesticide to 300 ng/ml. There was no cross-reactivity between the three analytes. Using a 96-well microplate and an autosampler, as many as 288 separate analyses can be completed in ~120 min with precision, sensitivity, and specificity equivalent to, if not better, than that found when these same analytes are measured by CIM or EIA.
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Bresnahan GA, Manthey FA, Howatt KA and Chakraborty M (2003) J Agr Food Chem 51:4004
Hines CJ, Deddens JA, Striley CA, Biagini RE, Shoemaker DA, Brown KK, Mackenzie BA, Hull RD (2003) Ann Occup Hyg 47:503
Biagini RE, Murphy DM, Sammons DL, Smith JP, Striley CAF, MacKenzie BA (2002) Bull Environ Contam Toxicol 68:470
Striley CAF, Biagini RE, Mastin JP, MacKenzie BA, Robertson SK (1999) Anal Chim Acta 399:109
Ashton F (1991) In: Ashton F, Monaco T, Barrett M (eds) Weed science: principles and practice. Wiley, New York
Hines CJ, Deddens JA, Striley CA, Biagini RE, Shoemaker DA, Brown KK, Mackenzie BA, Hull RD (2003) Ann Occup Hyg 47:503
Sanderson WT, Ringenburg V, Biagini R (1995) Am Ind Hyg Assoc J 56:890
Sanderson WT, Biagini R, Tolos W, Henningsen G, MacKenzie B (1995) Am Ind Hyg Assoc J 56:883
Fenske RA, Lu C (1994) Am Ind Hyg Assoc J 55:425
Lu C, Fenske RA (1999) Environ Health Persp 107:463
Mastin JP, Striley CAF, Biagini RE, Hines CJ, Hull RD, MacKenzie BA, Robertson SK (1998) Anal Chim Acta 376:119
Hryhorczuk DO, Moomey M, Burton A, Runkle K, Chen E, Saxer T, Slightom J, Dimos J, McCann K, Barr D (2002) Environ Health Persp 110 (Suppl 6):1041
Smith PA, Thompson MJ, Edwards JW (2002) J Chromatogr B 778:113
Lyubimov AV, Garry VF, Carlson RE, Barr DB, Baker SE (2000) J Lab Clin Med 136:116
Jaeger LL, Jones AD, Hammock BD (1998) Chem Res Toxicol 11:342
Biagini RE, Tolos W, Sanderson WT, Henningsen GM, MacKenzie B (1995) Bull Environ Contam Toxicol 54:245
Biagini RE, Schlottmann SA, Sammons DL, Smith JP, Snawder JC, Striley CA, MacKenzie BA, Weissman DN (2003) Clin Diagn Lab Immunol 10:744
Biagini RE, Sammons DL, Smith JP, MacKenzie BA, Striley CA, Semenova V, Steward-Clark E, Stamey K, Freeman AE, Quinn CP, Snawder JE (2004) Clin Diagn Lab Immunol 11:50
Rubio F, Veldhuis LJ, Clegg BS, Fleeker JR, Hall JC (2003) J Agr Food Chem 51:691
Vignali DA (2000) J Immunol Methods 243:243
Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr (1997) Clin Chem 43:1749
Blondell J (1997) Occup Med 12:209
Richter ED, Chlamtac N (2002) Int J Occup Environ Health 8:63
Baker SE, Barr DB, Driskell WJ, Beeson MD, Needham LL (2000) J Expo Anal Environ Epidemiol 10:789
Driskell WJ, Hill RH Jr (1997) Bull Environ Contam Toxicol 58:929
Pozzebon JM, Vilegas W, Jardim IC (2003) J Chromatogr A 987:375
Oliver K, Kettman J, Fulton R (1998) Clin Chem 44:2057
Nix B, Wild D (2001) In: Wild D (ed) The immunoassay handbook. Nature, New York
Barr DB, Barr JR, Maggio VL, Whitehead RD Jr, Sadowski MA, Whyatt RM, Needham LL (2002) J Chromatogr B 778:99
Carabias-Martýnez R, Garcýa-Hermida C, Rodrýguez-Gonzalo E, Soriano-Bravo F, Hernandez-Mendez J (2003) J Chromatogr A 1002:1
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Mention of a product or company name does not constitute endorsement by NIOSH. This work was supported in part by an interagency agreement between NIOSH and NIEHS (Y02ES10189).
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Biagini, R.E., Smith, J.P., Sammons, D.L. et al. Development of a sensitivity enhanced multiplexed fluorescence covalent microbead immunosorbent assay (FCMIA) for the measurement of glyphosate, atrazine and metolachlor mercapturate in water and urine. Anal Bioanal Chem 379, 368–374 (2004). https://doi.org/10.1007/s00216-004-2628-8
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DOI: https://doi.org/10.1007/s00216-004-2628-8