Abstract
The development of in vitro methods for screening potentially harmful biological activities of new compounds is an extremely important way to increase not only their intrinsic environmental safety, but also the public perception of the safety standards associated with them. In this work we use two yeast systems to test the ability of different chemicals to bind and activate two vertebrate receptors which are intimately related to adverse biological effects of pollution in exposed fauna: the estrogen receptor (ER) and the aryl hydrocarbon receptor (AhR). The panel of compounds analysed here includes well-known pollutants, like PCBs, pp′-DDT and hexachlorobenzene, together with the less-known, emerging putative pollutants, such as Sea-Nine, Irgarol and diuron. Results show the ability of some of these compounds to interact with one or both receptors, provide hints about the relationship between structure and activity, and suggest mechanistic explanations for the biological activities already described in whole-animal experiments. In addition, we show that AhR may have an intrinsic ligand promiscuity comparable to that of ER, a feature not fully appreciated in the past due to the technical difficulties involved with testing highly lipophilic substances in yeast-based assays.
Similar content being viewed by others
References
Colborn T (1995) Environ Health Perspect 103:135–136
Mangelsdorf DJ, Thummel C, Beato M, Herrlich P, Schutz G, Umesono K, Blumberg B, Kastner P, Mark M, Chambon P, Evans RM (1995) Cell 83:835–839
Colborn T, vom Saal FS, Soto AM (1993) Environ Health Perspect 101:378–384
Coldham NG, Dave M, Sivapathasundaram S, McDonnell DP, Connor C, Sauer MJ (1997) Environ Health Perspect 105:734–742
Elsby R, Ashby J, Sumpter JP, Brooks AN, Pennie WD, Maggs JL, Lefevre PA, Odum J, Beresford N, Paton D, Park BK (2000) Biochem Pharmacol 60:1519–1530
Scippo ML, Argiris C, Van De Weerdt C, Muller M, Willemsen P, Martial J, Maghuin-Rogister G (2004) Anal Bioanal Chem 378:664–669
Céspedes R, Petrovic M, Raldúa D, Saura U, Piña B, Lacorte S, Viana P, Barceló D (2004) Anal Bioanal Chem 378:697–708
Solé M, Raldúa D, Barceló D, Porte C (2003) Ecotoxicol Environ Safe 56:373–380
Solé M, Raldúa D, Piferrer F, Barceló D, Porte C (2003) Comp Biochem Phys C 136:145–156
Wester PW, van der Ven LT, Vos JG (2004) Toxicology 205:27–32
Mills LJ, Chichester C (2005) Sci Total Environ 343:1–34
Safe S (2005) Trends Endocrinol Metab 16:139–144
Hahn ME, Karchner SI, Shapiro MA, Perera SA (1997) Proc Natl Acad Sci USA 94:13743–13748
Nebert DW, Puga A, Vasiliou V (1993) Ann N Y Acad Sci 685:624–640
Sutter TR, Tang YM, Hayes CL, Wo YY, Jabs EW, Li X, Yin H, Cody CW, Greenlee WF (1994) J Biol Chem 269:13092–13099
Gonzalez FJ, Fernandez-Salguero P (1998) Drug Metab Dispos 26:1194–1198
Shimizu Y, Nakatsuru Y, Ichinose M, Takahashi Y, Kume H, Mimura J, Fujii-Kuriyama Y, Ishikawa T (2000) Proc Natl Acad Sci USA 97:779–782
Abbott BD, Perdew GH, Birnbaum LS (1994) Toxicol Appl Pharm 126:16–25
Poland A, Knutson JC (1982) Annu Rev Pharmacol 22:517–554
Connor K, Ramamoorthy K, Moore M, Mustain M, Chen I, Safe S, Zacharewski T, Gillesby B, Joyeux A, Balaguer P (1997) Toxicol Appl Pharm 145(1):111–123
Garcia-Reyero N, Grau E, Castillo M, Lopez de Alda MJ, Barceló D, Piña B (2001) Environ Toxicol Chem 20:1152–1158
Andersen H, Andersson A, Arnold S, Autrup H, Barfoed M, Beresford N, Bjerregaard P, Christiansen L, Gissel B, Hummel R, Jorgensen E, Korsgaard B, LeGuevel R, Leffers H, McLachlan J, Moller A, Nielsen J, Olea N, Oles-Karasko A, Pakdel F, Pedersen K, Perez P, Skakkeboek N, Sonnenschein C, Soto A, Sumpter J, Thorpe S, Grandjean P (1999) Environ Health Perspect 107:89–108
Bovee T, Heskamp H, Hamers A, Hoogenboom R, Nielen M (2005) Anal Chem Acta 529:57–64
Feldman D, Krishnan A (1995) Environ Health Perspect 105:129–133
Thomas K, Hurst M, Matthiessen P, Waldock M (2001) Environm Toxicol Chem 20:2165–2170
Quirós L, Céspedes R, Lacorte S, Viana P, Raldúa D, Barceló D, Piña B (2005) Environ Toxicol Chem 24:389–395
Gaido KW, Leonard LS, Lovell S, Gould JC, Babai D, Portier CJ, McDonnell DP (1997) Toxicol Appl Pharm 143:205–212
Nishihara T, Nishikawa J, Kanayama T, Dakeyama F, Saito K, Imagawa M, Takatori S, Kitagawa Y, Hori S, Utsumi H (2000) J Health Sci 46:282–298
Garcia-Reyero N, Requena V, Petrovic M, Fischer B, Hansen P, Barceló D, Piña B (2004) Environ Toxicol Chem 23:705–711
Arulmozhiraja S, Shiraishi F, Okumura T, Iida M, Takigami H, Edmonds J, Morita M (2005) Toxicol Sci 84:49–62
Green S, Chambon P (1991) In: Parker MG (ed) Nuclear hormone receptors. Academic, London pp 15–38
Schneider JC, Guarente L (1991) Methods Enzymol 194:373–388
Miller C (1999) Toxicol Appl Pharm 160:297–303
Noguerol T, Boronat S, Jarque S, Barceló D, Piña B (2006) Talanta 69:358–359
Vezina C, Walker N, Olson R (2004) Environ Health Perspect 112:1636–1644
Bemis JC, Nazarenko DA, Gasiewicz TA (2005) Toxicol Sci 87:529–536
Matthews J, Zacharewski T (2000) Toxicol Sci 53:326–339
Hestermann EV, Stegeman JJ, Hahn ME (2000) Toxicol Appl Pharmacol 168:160–172
Brown MM, Schneider UA, Petrulis JR, Bunce NJ (1994) Toxicol Appl Pharmacol 129:243–251
Denison MS, Nagy SR (2003) Annu Rev Pharmacol Toxicol 43:309–334
Garcia-Reyero N, Piña B, Grimalt JO, Fernandez P, Fonts R, Polvillo O, Martrat B (2005) Environ Sci Technol 39:1427–1435
Aoki Y (2001) Environ Res 86:2–11
Navas JM, Segner H (2000) Aquat Toxicol 51:79–92
Jacobs MN, Dickins M, Lewis DF (2003) J Steroid Biochem Mol Biol 84:117–132
Pike AC, Brzozowski AM, Hubbard RE (2000) J Steroid Biochem Mol Biol 74:261–268
Shibata H, Spencer TE, Oñate SA, Jenster G, Tsai SY, Tsai MJ, O’Malley BW (1997) Recent Prog Horm Res 14:141–164
Mak HY, Hoare S, Henttu PM, Parker MG (1999) Mol Cell Biol 19:3895–3903
Edwards DP (2000) J Mammary Gland Biol 5:307–324
Shiraishi F, Okumura T, Nomachi M, Serizawa S, Nishikawa J, Edmonds JS, Shiraishi H, Morita M (2003) Chemosphere 52:33–42
Layton AC, Sanseverino J, Gregory BW, Easter JP, Sayler GS, Schultz TW (2002) Toxicol Appl Pharmacol 180:157–163
Frydenvang K, Greenwood J, Vogensen S, Brehm L (2002) Struct Chem 13:479–490
Kelce WR, Stone CR, Laws SC, Gray LE, Kemppainen JA, Wilson EM (1995) Nature 375:581–585
Schriks M, Vrabie CM, Gutleb AC, Faassen EJ, Rietjens IM, Murk AJ (2006) Toxicol In Vitro 20:490–498
DeVito M, Biegel L, Brouwer A, Brown S, Brucker-Davis F, Cheek AO, Christensen R, Colborn T, Cooke P, Crissman J, Crofton K, Doerge D, Gray E, Hauser P, Hurley P, Kohn M, Lazar J, McMaster S, McClain M, McConnell E, Meier C, Miller R, Tietge J, Tyl R (1999) Environ Health Perspect 107:407–415
Acknowledgements
This work has been supported by the Spanish Ministry for Science and Technology (SAF2002-00371), the Spanish Ministry of Science and Education (BIO2005-00840, CTM2005-24255-E) and the European Union (SWIFT-WFD, SSPI-CT-2003-502492). This work reflects only the authors’ views and the European Community is not liable for any use that may be made of the information contained therein.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Noguerol, TN., Boronat, S., Casado, M. et al. Evaluating the interactions of vertebrate receptors with persistent pollutants and antifouling pesticides using recombinant yeast assays. Anal Bioanal Chem 385, 1012–1019 (2006). https://doi.org/10.1007/s00216-006-0476-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-006-0476-4