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Detection of V-type nerve agent degradation products at electrodes modified by PPy/PQQ using CaCl2 as supporting electrolyte

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Abstract

Electrochemical detection without derivatization was used to detect thiol-containing degradation products of V-type nerve agents. Electropolymerization of pyrrole was used for entrapment of the biocatalyst PQQ to produce a sensor. Various parameters which affect the detection processes such as the type of the supporting electrolyte used during electrodeposition and the thickness of the polypyrrole film were examined and optimized. Electocatalytic oxidation of thiols by the PPy/PQQ electrode was strongly affected by the presence of Ca2+ cations during electrodeposition of the PPy/PQQ. Cyclic voltammetry, linear sweep voltammetry and amperometry have been used for electrode characterization. Amperometric detection of the V-type nerve agent thiol degradation products 2-(dimethylamino)ethanethiol (DMAET) and 2-(diethylamino)ethanethiol (DEAET) was performed at 0.38 V. Linear calibration plots were observed for these compounds. The detection limits of 4.5 and 3 μM were obtained for DMAET and DEAET respectively, with sensitivities of 1.18 and 1.37 nA μM−1 cm−2.

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Abbreviations

CE:

capillary electrophoresis

CV:

cyclic voltammetry

CWA:

chemical warfare agents

DEAET:

2-(diethylamino)ethanethiol

DMAET:

2-(dimethylamino)ethanethiol

EC:

electrochemical

GC:

glassy carbon

GC-MS:

gas chromatography with mass spectrometry detection

HPLC:

high performance liquid chromatography

LSV:

linear sweep voltammetry

OP:

organophosphate

PQQ:

pyrroloquinoline quinone

PPy:

polypyrrole

SEM:

scanning electron microscopy

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Authors and Affiliations

  1. Department of Chemistry, University of Montana, Missoula, MT, 59812, USA

    Olga V. Shulga & Christopher Palmer

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  1. Olga V. Shulga
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  2. Christopher Palmer
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Correspondence to Olga V. Shulga.

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Shulga, O.V., Palmer, C. Detection of V-type nerve agent degradation products at electrodes modified by PPy/PQQ using CaCl2 as supporting electrolyte. Anal Bioanal Chem 385, 1116–1123 (2006). https://doi.org/10.1007/s00216-006-0531-1

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  • DOI: https://doi.org/10.1007/s00216-006-0531-1

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