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Human urine certified reference material CZ 6010: creatinine and toluene metabolites (hippuric acid and o-cresol) and a benzene metabolite (phenol)

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Abstract

A reference material for the biological monitoring of occupational exposure to toluene, benzene and phenol was prepared. O-cresol and hippuric acid (metabolites of toluene) are used for the biological monitoring of occupational exposure to toluene. Phenol, a metabolite of benzene, is used for the biological monitoring of exposure to benzene, but phenol can of course also be used as an indicator of exposure to phenol as well. The reference material (RM) used for the determination of these metabolites was prepared by freeze-drying pooled urine samples obtained from healthy persons occupationally exposed to toluene and those taking part in an inhalation experiment. Tests for homogeneity and stability were performed by determining urine concentrations of o-cresol, hippuric acid, creatinine and phenol. To investigate the stability of the RM, the urinary concentrations of o-cresol and phenol were monitored for eighteen months using GC and HPLC, while those of hippuric acid and creatinine were followed for five and six years, respectively, using HPLC. Analysis of variance showed that the concentrations did not change. The certified concentration values (and their uncertainties) of the substances in this reference material (phenol concentration c=6.46±0.58 mg l−1; o-cresol concentration c=1.17±0.15 mg l−1; hippuric acid concentration c=1328±30 mg l−1; creatinine concentration c=0.82±0.10 g l−1) were evaluated via the interactive statistical programme IPECA.

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References

  1. Pelclová D, Černá M, Pastorková A, Procházka B, Vrbíková V, Hurychová D, Dlasková Z, Hornychová M (2000) Arch Environ Health 55:268–273

    Article  Google Scholar 

  2. Hammer KD (2002) Mutat Res 519:171–177

    CAS  Google Scholar 

  3. MZ (2006) Register of Czech National Health Information System, Register of Work Categorization (KaPr), actualization 28.3.2006. Czech Ministry of Health (MZ), Prague (see http://www.Healthcareregisters.cz, last accessed 15th August 2006)

  4. Angerer J (1979) Int Arch Occup Environ Health 43:63–67

    Article  CAS  Google Scholar 

  5. Pierce CH, Chen YL, Hurtle W, Morgan M (2004) J Toxicol Environ Health A 67:1131–1158

    Google Scholar 

  6. Angerer J (1985) Int Arch Occup Environ Health 56:323–328

    Article  CAS  Google Scholar 

  7. Pierce CH, Chen Y, Dills R, Kalman DA, Morgan M (2002) Toxicol Lett 129:65–76

    Google Scholar 

  8. Truchon G, Tardif R, Brodeur J (1999) Appl Occup Environ Hyg 14:677–681

    Article  CAS  Google Scholar 

  9. Fujii T, Kawabe S, Horike T, Taguchi T, Ogata Mx (1999) J Chromatogr B 730:41–47

    CAS  Google Scholar 

  10. Laurens JB, Mbianda XY, Spies JH, Ubbink JB, Vermaak WJH (2002) J Chromatogr B 774:173–185

    Google Scholar 

  11. Angerer J, Schildbach M, Kramer A (1998) Arch Toxicol 72:119–123

    Article  CAS  Google Scholar 

  12. Inoue O, Kanno E, Yusa T, Kakizaki M, Ukai H, Okamoto S, Higashikawa K, Ikeda M (2002) Int Arch Occup Environ Health 75:341–347

    Article  CAS  Google Scholar 

  13. ACGIH (2002) Threshold limit values for chemical substances and physical agents and biological exposure indices. ACGIH, Cincinnati, OH, p 93

  14. Society of Occupational Medicine (2006) Online documents. Society of Occupational Medicine of the Czech Medical Association of Jan Evangelista Purkyne, Prague (documents available from http://www.pracovni-lekarstvi.cz/dokumenty.php, last accessed 15th August 2006)

  15. MZ (2003) Decree of the Ministry of Health No. 432/2003 Coll. Czech Ministry of Health (MZ), Prague

  16. Stokes P, O’Connor GJ (2003) J Chromatogr B 794:125–136

    Google Scholar 

  17. Šperlingová I, Dabrowská L, Kučera J, Tichý M (1995) Fresenius J Anal Chem 352:87–91

    Article  Google Scholar 

  18. ČMI (2002) Methodology 017 MP-C001-02. Czech Metrological Institute, Brno

  19. ISO (2006) Guide 35. ISO/REMCO, Geneva

  20. ISO (2003) Guide 34. ISO/REMCO, Geneva

  21. Schneiderka P, Pacáková V, Štulík K, Jelínková K (1993) J Chromatogr 614:221

    CAS  Google Scholar 

  22. Jaffe M (1886) Z Physiol Chem 10:391

    Google Scholar 

  23. Šperlingová I, Dabrowská L, Stránský V, Tichý M (2004) Anal Bioanal Chem 378:536–543

    Article  CAS  Google Scholar 

  24. Šperlingová I, Dabrowská L, Stránský V, Kučera J, Tichý M (2006) Accred Qual Assur 11:231–235

    Article  CAS  Google Scholar 

  25. Kučera J, Faltejsek J (1995) Fresenius J Anal Chem 352:80–86

    Article  Google Scholar 

Download references

Acknowledgement

This work was supported by the Internal Grant Agency of Ministry of Health of the Czech Republic (Grant NR/7831-3).

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Authors and Affiliations

  1. Department of Toxicological Analysis, National Institute of Public Health, Šrobárova 48, 100 42, Praha, Czech Republic

    I. Šperlingová, L. Dabrowská, V. Stránský & M. Tichý

  2. Academy of Science of Czech Republic, Nuclear Physics Institute, 250 68, Řež near Praha, Czech Republic

    J. Kučera

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  1. I. Šperlingová
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  2. L. Dabrowská
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  3. V. Stránský
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  4. J. Kučera
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  5. M. Tichý
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Correspondence to I. Šperlingová.

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Šperlingová, I., Dabrowská, L., Stránský, V. et al. Human urine certified reference material CZ 6010: creatinine and toluene metabolites (hippuric acid and o-cresol) and a benzene metabolite (phenol). Anal Bioanal Chem 387, 2419–2424 (2007). https://doi.org/10.1007/s00216-006-0708-7

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  • DOI: https://doi.org/10.1007/s00216-006-0708-7

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