Abstract
An electroenzymatic glucose sensor based on a simple enzyme immobilization technique was constructed and tested. The glucose sensor measures glucose concentrations as changes of oxygen concentrations induced by enzymatic reactions. The immobilizing procedure was developed with the purpose of producing wearable biosensors for clinical use. Two types of biocompatible polymers, 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with dodecyl methacrylate (PMD) and MPC copolymerized with 2-ethylhexyl methacrylat, were compared as a sensitive membrane of biosensors. The PMD enzyme membrane had a better response time. Linearity, reproducibility, effect of the concentrations of immobilized enzyme and drifts of sensor characteristics in long-term tests were also investigated. The linear characteristics were confirmed with glucose concentration from 0.01 to 2.00 mmol/l, with a coefficient of determination of 0.9999. The average output current for 1 mmol/l and the standard deviation were 0.992 and 0.0283 μA. Significant changes in the sensor's characteristics were not observed for 2 weeks when it was kept in a refrigerator at 4 °C. Because of the simple procedure, the enzyme immobilization method is not only useful for wearable devices but also other devices such as micro total analysis systems.
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References
Pickup JC, Williams G (2003) Textbook of diabetes, 3rd edn. Blackwell, Oxford
Pickup JC, Hussain F, Evans ND, Sachedina N (2005) Biosens Bioelectron 20:1897–1902
Badugu R, Lakowicz JR, Geddes CD (2003) J Fluoresc 13:371–374
March WF, Rabinovitch B, Adams R, Wise JR, Melton M (1982) Trans Am Soc Artif Intern Organs 28:232–235
Rabinovitch B, March WF, Adams RL (1982) Diabetes Care 5:254–258
Schier GM, Moses RG, Gan ET, Blair SC (1988) Diabetes Res Clin Pract 4:177–181
Clarke W, Becker DJ, Cox D, Santiago JV, White NH, Betschart J, Eckenrode K, Levandoski LA, Prusinki EA, Simineiro LM, Snyder AL, Tideman AM, Yaegar T (1988) Diabetes Res Clin Pract 4:209–214
Meadows D, Schultz JS (1988) Talanta 35:145–150
Trettnak W, Wolfbeis OS (1989) Anal Chim Acta 221:195–203
Heise HM, Marbach R, Koschinsky TH, Gries FA (1994) Ann Occup Hyg 18:439–447
Tolosa L, Malak H, Rao G, Lakowicz JR (1997) Sens Actuators B 45:93–99
Tolosa L, Gryczynski I, Eichorn LR, Dattelbaum JD, Castellano FN, Rao G, Lakowicz JR (1999) Anal Biochem 267, 114–120
D’Auria S, Dicesare N, Gryczynski Z, Gryczynski I, Rossi M, Lakowicz JR (2000) Biochem Biophys Res Commun 274:727–731
Bailey TS, Zisser HC, Garg SK (2007) Diabetes Technol Ther 9:203–210
Garg SK, Schwartz S, Edelman SV (2007) Diabetes Care 27:734–738
Vishwanathan V (2002) J Indian Med Assoc 100:181–183
Wilson GS, Gifford R (2005) Biosens Bioelectron 20:2388–2403
Daum KM, Hill RM (1982) Invest Ophthalmol Vis Sci 22:509–515
Man SFP, Adams GK, Proctor DF (1979) J Appl Physiol Respir Environ Exerc Physiol 46:205–211
Mitsubayashi K, Suzuki M, Tamiya E, Karube I (1994) Anal Chim Acta 289:27–34
Romano A, Rolant F (1988) Metab Pediatr Syst Ophthalmol 11:78–85
Mitsubayashi K, Wakabayashi Y, Tanimoto S, Murotomi D (2003) Biosens Bioelectron 19:67–71
Iguchi S, Kudo H, Saito T, Ogawa M, Saito H, Otsuka K, Funakubo A, Mitsubayashi K (2007) Biomed Microdevices 9:603–609
Kudo H, Sawada T, Kazawa E, Yoshida H, Iwasaki Y, Mitsubayashi K (2006) Biosens Bioelectron 22:558–562
Chen R, Jin Z, Colon LA (1996) J Capillary Electrophor 3:243–248
Jin Z, Chen R, Colon LA (1997) Anal Chem 69:1326–1331
Sen DK, Sarin GS (1980) Br J Ophthalmol 64:693–699
March WF, Smith FE, Herbrechtsmeier P, Domschke A, Morris C (2001) Diabetes 50(Suppl.):A125
Ishihara K, Ueda T, Nakabayashi N (1990) Polym J 22:355–360
Ishihara K, Nomura H, Mihara T, Kurita K, Iwasaki Y, Nakabayashi N (1998) J Biomed Mater Res 39:323–330
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This work was partly supported by the Japan Society for the Promotion of Science (JSPS) and the Nakatani Foundation of Electronic Measuring Technology Advancement.
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Kudo, H., Yagi, T., Chu, M.X. et al. Glucose sensor using a phospholipid polymer-based enzyme immobilization method. Anal Bioanal Chem 391, 1269–1274 (2008). https://doi.org/10.1007/s00216-007-1824-8
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DOI: https://doi.org/10.1007/s00216-007-1824-8