Skip to main content
SpringerLink
Log in

PowerSlicing to determine fluorescence lifetimes of water-soluble organic matter derived from soils, plant biomass, and animal manures

  • Technical Notes
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Time-resolved fluorescence spectroscopy was used to characterize water-soluble organic matter (WSOM) which plays an important role in soil ecosystem processes. WSOM was extracted from plant biomass, animal manures, and soils from controlled cropping systems studies with known histories of organic amendments. Lifetime constants were derived using the multi-way PowerSlicing method which provides a non-iterative, multi-exponential fitting of decay profiles. The lifetimes obtained by PowerSlicing were not significantly different from those obtained using the traditional discrete components analysis. The three components attributed to WSOM had lifetimes of 0.38 ± 0.14, 2.11 ± 0.72, and 7.08 ± 1.18 ns which are in agreement with previous lifetimes reported for humic substances. This study provides further support for the new paradigm for the structure of soil organic matter where the organic matter is composed of low-molecular-weight components held together by hydrogen bonding and hydrophobic interactions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Zsolnay A (1996) In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier, Amsterdam

    Google Scholar 

  2. Morel F, Hering JG (1993) Principles and applications of aquatic chemistry. John Wiley & Sons, New York

    Google Scholar 

  3. Stevenson FJ (1994) Humus chemistry: genesis, composition, reactions. John Wiley & Sons, New York

    Google Scholar 

  4. Senesi N (1990) Anal Chim Acta 232:77–106

    Article  CAS  Google Scholar 

  5. Clark CD, Jimenez-Morais J, Jones G, Zanardi-Lamardo E, Moore CA, Zika RG (2002) Marine Chem 78:121–135

    Article  CAS  Google Scholar 

  6. Cook RL, Langford CH (1995) Anal Chem 67:174–180

    Article  CAS  Google Scholar 

  7. Kumke MU, Tiseanu C, Abbt-Braun G, Frimmel FH (1998) J Fluorescence 8:309–318

    Article  CAS  Google Scholar 

  8. McGown LB, Hemmingsen SL, Shaver JM, Geng L (1995) Appl Spectrosc 49:60–66

    Article  CAS  Google Scholar 

  9. Engelsen SB, Bro R (2003) J Magn Reson 163:192–197

    Article  CAS  Google Scholar 

  10. Ohno T, Bro R (2006) Soil Sci Soc Am J 70:2028–2037

    Article  CAS  Google Scholar 

  11. Zhou LX, Wong JWC (2000) J Environ Qual 29:1852–1856

    Article  CAS  Google Scholar 

  12. Ainsworth CC, Friedrich DM, Gassman PL, Wang Z, Joly AG (1998) Geochimca 62:595–612

    CAS  Google Scholar 

  13. Holtom GR (1990) SPIE 1204:2–12

    Article  Google Scholar 

  14. Sidiropoulos ND, Bro R (2000) J Chemometr 14:229–239

    Article  CAS  Google Scholar 

  15. Harshman RA (1970) UCLA Working Papers in Phonetics 16:1–84

    Google Scholar 

  16. Bro R (1997) Chemometr Intell Lab 38:149–171

    Article  CAS  Google Scholar 

  17. Stedmon CA, Markager S, Bro R (2003) Marine Chem 82:239–254

    Article  CAS  Google Scholar 

  18. Sutton R, Sposito G (2005) Environ Sci Technol 39:9009–9015

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This project was supported by National Research Initiative Competitive Grant no. 2003–35107–13628 from the USDA Cooperative State Research, Education, and Extension Service, and has also been supported by Hatch funds provided by the Maine Agricultural and Forest Experiment Station. Part of this research was performed at the W.R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility at PNNL managed by the Department of Energy’s Office of Biological and Environmental Research. Pacific Northwest National Laboratory is operated for the US Department of Energy by Battelle under Contract DE-AC06–76RLO 1830. This is journal number 2995 of the Maine Agricultural and Forest Experiment Station.

Author information

Authors and Affiliations

  1. Department of Plant, Soil, and Environmental Sciences, University of Maine, 5722 Deering Hall, Orono, ME, 04469–5722, USA

    Tsutomu Ohno

  2. Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, 902 Battelle Blvd, Mail Stop K8–96, Richland, WA, 99352, USA

    Zheming Wang

  3. Faculty of Life Sciences, Department of Food Science, University of Copenhagen, Rolighedsvej 30, 1958, Frederiksberg C, Denmark

    Rasmus Bro

Authors
  1. Tsutomu Ohno
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zheming Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rasmus Bro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tsutomu Ohno.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohno, T., Wang, Z. & Bro, R. PowerSlicing to determine fluorescence lifetimes of water-soluble organic matter derived from soils, plant biomass, and animal manures. Anal Bioanal Chem 390, 2189–2194 (2008). https://doi.org/10.1007/s00216-008-1963-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-008-1963-6

Keywords

Search

Navigation