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Multiresidue analysis of atrazine, diuron and their degradation products in sewage sludge by liquid chromatography tandem mass spectrometry

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Abstract

A multiresidue method has been developed to analyze atrazine (ATZ), diuron (DIU), and their major degradation products, desethylatrazine (DEA), desisopropylatrazine (DIA), and dichlorophenylmethylurea in sewage sludge. Liquid chromatography coupled to electrospray tandem mass spectrometry (LC–ESI-MS–MS) allowed, in the multiple-reaction monitoring mode, the simultaneous analysis of these pesticides in only one run after their extraction with ethyl acetate–dichloromethane 90:10 (v/v) and a cleanup on a Florisil column. Stable isotopically labeled ATZ and DIU were used as internal standards to overcome matrix effects during the pesticide quantification. Using fortified samples, the method gave rise to 86–115% as mean recovery values depending on the analyte. Limits of detection (LODs) and of quantification (LOQs) ranging from 0.3 (DIA) to 1.5 (DEA) μg kg−1 dw and from 0.4 (DIA) to 2.0 (DEA) μg kg−1 dw, respectively, were sufficient to achieve the monitoring of these molecules in sludge from wastewater treatment plants of the Ile-de-France region.

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Acknowledgements

The authors are grateful to the French Agency for Environment and Energy Management (ADEME) and to the National Institute for Agricultural Research (INRA) for the financial support of Project 0275048 and the PhD fellowship of A.G. They also thank the WWTPs for providing sludge samples.

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Authors and Affiliations

  1. INRA, UR258 Phytopharmacie et Médiateurs Chimiques, Route de Saint-Cyr, 78026, Versailles Cedex, France

    Aline Ghanem, Philippe Bados, François Perreau, Rachid Benabdallah & Jacques Einhorn

  2. INRA, UR251 Physicochimie et Ecotoxicologie des Sols d’Agrosystèmes Contaminés, Route de Saint-Cyr, 78026, Versailles Cedex, France

    François Perreau & Rachid Benabdallah

  3. Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut des Sciences et Technologies de l’Environnement (ENAC ISTE GE), 1015, Lausanne, Switzerland

    Cécile Plagellat & Luiz Felippe de Alencastro

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  1. Aline Ghanem
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  2. Philippe Bados
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  3. François Perreau
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  4. Rachid Benabdallah
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  5. Cécile Plagellat
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  6. Luiz Felippe de Alencastro
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  7. Jacques Einhorn
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Corresponding author

Correspondence to Jacques Einhorn.

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Ghanem, A., Bados, P., Perreau, F. et al. Multiresidue analysis of atrazine, diuron and their degradation products in sewage sludge by liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 391, 345–352 (2008). https://doi.org/10.1007/s00216-008-2007-y

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  • DOI: https://doi.org/10.1007/s00216-008-2007-y

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