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Ultra high performance liquid chromatographic-tandem mass spectrometric multi-analyte procedure for target screening and quantification in human blood plasma: validation and application for 31 neuroleptics, 28 benzodiazepines, and Z-drugs

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Abstract

For fast and reliable screening, identification, and quantification of as many analytes as possible, multi-analyte approaches are very useful in clinical and forensic toxicology. Using ultra high performance liquid chromatography-tandem mass spectrometry, such an approach has been developed for blood plasma analysis after simple liquid–liquid extraction. In the present paper, validation and application is described for 31 neuroleptics, 28 benzodiazepines, and Z-drugs (zaleplone, zolpidem, and zopiclone). The validation parameters included recovery, matrix effects, process efficiency, ion suppression/enhancement of co-eluting analytes, selectivity, crosstalk, accuracy and precision, stabilities, and limits of quantification and detection. The results showed that the approach was selective, sensitive, accurate, and precise for 24 neuroleptics and 21 benzodiazepines and Z-drugs. The remaining analytes were unstable and/or too low dosed. Cost- and time-saving one-point calibration was applicable only for half of the analytes. The applicability was successfully shown for most of the drugs by analyzing authentic plasma samples and external quality control samples.

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References

  1. Maurer HH (2007) Anal Bioanal Chem 388:1315–1325

    Article  CAS  Google Scholar 

  2. Maurer HH (2009) Anal Bioanal Chem 393:97–107

    Article  CAS  Google Scholar 

  3. Peters FT (2010) Clin Biochem 44:54–65

    Article  Google Scholar 

  4. Remane D, Meyer MR, Peters FT, Wissenbach DK, Maurer HH (2010) Anal Bioanal Chem 397:2303–2314

    Article  CAS  Google Scholar 

  5. Kirchherr H, Kuhn-Velten WN (2006) J Chromatogr B Analyt Technol Biomed Life Sci 843:100–113

    Article  CAS  Google Scholar 

  6. Bassan DM, Erdmann F, Krull R (2011) Anal Bioanal Chem 400:43–50

    Article  CAS  Google Scholar 

  7. Remane D, Meyer MR, Wissenbach DK, Maurer HH (2010) Rapid Commun Mass Spectrom 24:3103–3108

    Article  CAS  Google Scholar 

  8. Remane D, Wissenbach DK, Meyer MR, Maurer HH (2010) Rapid Commun Mass Spectrom 24:859–867

    Article  CAS  Google Scholar 

  9. Remane D, Meyer MR, Wissenbach DK, Maurer HH (2011) Anal Bioanal Chem 400:2093–2107

    Article  CAS  Google Scholar 

  10. Peters FT, Paul LD, Musshoff F, Aebi B, Auwaerter V, Kraemer T, Skopp G (2009) Toxichem Krimtech 76:185–208. Available at http://www.gtfch.org/cms/index.php/guidelines

    Google Scholar 

  11. Shah VP, Midha KK, Findlay JW, Hill HM, Hulse JD, McGilveray IJ, McKay G, Miller KJ, Patnaik RN, Powell ML, Tonelli A, Viswanathan CT, Yacobi A (2000) Pharm Res 17:1551–1557

    Article  CAS  Google Scholar 

  12. NCCLS (1999) Evaluation of precision performance of clinical chemistry devices; approved guideline. NCCLS, Wayne

    Google Scholar 

  13. Peters FT, Drummer OH, Musshoff F (2007) Forensic Sci Int 165:216–224

    Article  CAS  Google Scholar 

  14. Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030

    Article  CAS  Google Scholar 

  15. Baumann P, Hiemke C, Ulrich S, Eckermann G, Gaertner I, Gerlach M, Kuss HJ, Laux G, Muller-Oerlinghausen B, Rao ML, Riederer P, Zernig G (2004) Pharmacopsychiatry 37:243–265

    Article  CAS  Google Scholar 

  16. Schulz M, Schmoldt A (2003) Pharmazie 58:447–474

    CAS  Google Scholar 

  17. Brunner, E., Dassinger M, Grohmann I, Jung B, Kuhlmann A, Loewe M, Pfleger A, Schwinn A, Selz C, Sieprath S, Sy K (2010) FachInfo-Service. Rote Liste Service, Frankfurt. Available at http://www.fachinfo.de

  18. Peters FT, Paul LD, Musshoff F, Aebi B, Auwaerter V, Kraemer T, Skopp G (2009) Toxichem Krimtech 76:185–208. Available at https://www.gtfch.org/cms/files/GTFCh_Richtlinie_Anhang%20B_Validierung_Version%201.pdf

    Google Scholar 

Download references

Acknowledgements

The authors thank Andrea E. Schwaninger, Gabi Ulrich, Carsten Schröder, and Armin A. Weber for their helpful discussions as well as Kornelia Weidemann and Dr. Edeltraud Thiry, ThermoFisher Scientific, Scientific Instruments, Dreieich (Germany) for their support.

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Authors and Affiliations

  1. Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg, Saar, Germany

    Daniela Remane, Markus R. Meyer, Dirk K. Wissenbach & Hans H. Maurer

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  1. Daniela Remane
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  2. Markus R. Meyer
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  3. Dirk K. Wissenbach
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  4. Hans H. Maurer
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Correspondence to Hans H. Maurer.

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Remane, D., Meyer, M.R., Wissenbach, D.K. et al. Ultra high performance liquid chromatographic-tandem mass spectrometric multi-analyte procedure for target screening and quantification in human blood plasma: validation and application for 31 neuroleptics, 28 benzodiazepines, and Z-drugs. Anal Bioanal Chem 401, 1341–1352 (2011). https://doi.org/10.1007/s00216-011-5187-9

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  • DOI: https://doi.org/10.1007/s00216-011-5187-9

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