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Direct surface plasmon resonance immunosensing of pyraclostrobin residues in untreated fruit juices

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Abstract

A surface plasmon resonance (SPR) immunoassay for on-line detection of the strobilurin fungicide pyraclostrobin in untreated fruit juices is presented. The analysis of pyraclostrobin residues is accomplished in apple, grape, and cranberry samples by monitoring the recognition events occurring separately in a two-channel home-made SPR biosensor. Covalent coupling of the analyte derivative results in a reversible method, enabling more than 80 measurements on the same sensor surface. Optimization of the immunoassay conditions provides limits of detection as low as 0.16 μg L−1. The selectivity and reproducibility of the analysis is ensured by studying both non-specific interactions with unrelated compounds and inter-assay coefficients of variation. Excellent recovery ranging from 98 to 103 % was achieved by a simple 1:5 dilution of fruit juice with assay buffer before the analysis. The lack of previous cleaning and homogenization procedures reduces the analysis time of a single food sample to only 25 min, including the regeneration cycle.

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Authors and Affiliations

  1. Institute of Food Science and Technology (ICTAL), University of León, 24007, León, Spain

    E. Mauriz & C. García-Fernández

  2. Institute of Agrochemistry and Food Technology, Spanish National Research Council, 46100, Burjassot, València, Spain

    J. V. Mercader & A. Abad-Fuentes

  3. IUMA, Institute for Applied Microelectronics, University of Las Palmas GC, Las Palmas GC, 35001, Spain

    A. M. Escuela

  4. Research Center on Nanoscience and Nanotechnology CIN2 (CSIC) and CIBER-BBN, Campus UAB, 08193, Bellaterra, Barcelona, Spain

    L. M. Lechuga

Authors
  1. E. Mauriz
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  2. C. García-Fernández
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  3. J. V. Mercader
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  4. A. Abad-Fuentes
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  5. A. M. Escuela
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  6. L. M. Lechuga
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Correspondence to E. Mauriz.

Additional information

Published in the special paper collection Optical Biochemical and Chemical Sensors with guest editor Laura M. Lechuga.

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Mauriz, E., García-Fernández, C., Mercader, J.V. et al. Direct surface plasmon resonance immunosensing of pyraclostrobin residues in untreated fruit juices. Anal Bioanal Chem 404, 2877–2886 (2012). https://doi.org/10.1007/s00216-012-6321-z

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  • DOI: https://doi.org/10.1007/s00216-012-6321-z

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