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Trace analysis of acetylcholinesterase inhibitors with antipsychotic drugs for Alzheimer’s disease by capillary electrophoresis with on column field-amplified sample injection

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Abstract

A simple and sensitive capillary zone electrophoresis (CZE) with UV detection (214 nm) was developed and validated for the simultaneous determination of the acetylcholinesterase inhibitors (AChEI), donepezil, and rivastigmine, with antipsychotic drugs in plasma. A sample pretreatment by liquid–liquid extraction and subsequent quantification by CZE with field-amplified sample injection (FASI) was used. The optimum separation for these analytes was achieved in <20 min at 25 °C with a fused-silica capillary column of 60.2 cm × 50 μm I.D. (effective length 50 cm) and a run buffer containing 120 mM phosphate (pH 4.0) with 0.1 % γ-cyclodextrin, 40 % methanol (MeOH), and 0.02 % polyvinyl alcohol as a dynamic coating to reduce analytes’ interaction with the capillary wall. Using phenformin as an internal standard (40.0 ng/mL), the linear ranges of the proposed method for the simultaneous determination of donepezil, rivastigmine, aripiprazole, quetiapine, risperidone, clozapine, ziprasidone, and trazodone were over the range 4.0–80.0 ng/mL, and olanzapine was over the range 1.0–20.0 ng/mL. The method was applied for concentrations monitoring of AChEIs and antipsychotic drugs in ten Alzheimer’s disease patients with behavioral and psychological symptoms of dementia after oral administration of the commercial products.

Electropherogram of tested chemicals in plasma determinations

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Acknowledgments

The authors are grateful to the National Science Council (NSC 99-2113-M-037-005-MY3) for financial support of the study.

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Authors and Affiliations

  1. School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan

    Yi-Rou Wang, Shun-Jin Lin & Su-Hwei Chen

  2. Department of Neurology, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan

    Yuan-Han Yang

  3. Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan

    Chi-Yu Lu

Authors
  1. Yi-Rou Wang
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  2. Yuan-Han Yang
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  3. Chi-Yu Lu
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  4. Shun-Jin Lin
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  5. Su-Hwei Chen
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Correspondence to Su-Hwei Chen.

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Wang, YR., Yang, YH., Lu, CY. et al. Trace analysis of acetylcholinesterase inhibitors with antipsychotic drugs for Alzheimer’s disease by capillary electrophoresis with on column field-amplified sample injection. Anal Bioanal Chem 405, 3233–3242 (2013). https://doi.org/10.1007/s00216-013-6767-7

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  • DOI: https://doi.org/10.1007/s00216-013-6767-7

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