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Electrochemical investigation of the interaction between lysozyme-shelled microbubbles and vitamin C

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Abstract

We report loading of vitamin C (ascorbic acid) on to lysozyme-shelled microbubbles. The interaction between lysozyme-shelled microbubbles and vitamin C was studied by use of cyclic and differential pulse voltammetry, zeta potential measurements, and scanning electron microscopy. The effect of microbubbles on electrochemical measurement of ascorbic acid was evaluated. The linear range for ascorbic acid obtained for differential pulse measurement in the presence of 1 mg mL−1 microbubbles was 1–50 μmol L−1 (y = 0.067x + 0.130, r 2 = 0.995), with a detection limit of 0.5 μmol L−1. The experimental conditions, i.e., pH and ionic strength, were optimized to improve the interaction between ascorbic acid and lysozyme-shelled microbubbles. The results were satisfactory when the interaction was performed for 1 h in aqueous solution at pH 6. The amount of vitamin C loaded on the microbubbles (90 % of the analyte added, RSD inter-expt. = 3 %, n = 6) and the stability of microbubbles–ascorbic acid complex (until 72 h at 25 °C) were also evaluated by use of differential pulse voltammetry and zeta potential measurements.

Schematic figure of the interaction between LSμB (positively charged) and ascorbic acid (negatively charged). Ultrasound (US) assisted breaking of the LSμB's shell causes the release of drug located on the surface of the microbubbles

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Acknowledgments

This work was funded by the Marie Curie IRSES 7° FP European Project 2009-2010 and supported by the University of Melbourne IPRS fellowship and Vic Govt. Virs fellowship.

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Authors and Affiliations

  1. Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma “Tor Vergata”, Via della Ricerca Scientifica, 00173, Rome, Italy

    Francesca Cavalieri, Laura Micheli, Mariarosaria Tortora & Giuseppe Palleschi

  2. School of Chemistry, The University of Melbourne, Melbourne, Victoria, 3010, Australia

    Francesca Cavalieri, Meifang Zhou & Muthupandian Ashokkumar

  3. Consorzio Interuniversitario Biostrutture e Biosistemi “INBB”, Viale delle Medaglie d’Oro 305, 00136, Rome, Italy

    Laura Micheli & Giuseppe Palleschi

  4. Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Muthupandian Ashokkumar

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  1. Francesca Cavalieri
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  2. Laura Micheli
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  3. Meifang Zhou
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  4. Mariarosaria Tortora
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  5. Giuseppe Palleschi
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  6. Muthupandian Ashokkumar
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Correspondence to Laura Micheli or Muthupandian Ashokkumar.

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Cavalieri, F., Micheli, L., Zhou, M. et al. Electrochemical investigation of the interaction between lysozyme-shelled microbubbles and vitamin C. Anal Bioanal Chem 405, 5531–5538 (2013). https://doi.org/10.1007/s00216-013-6895-0

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  • DOI: https://doi.org/10.1007/s00216-013-6895-0

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