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Quantification of moxifloxacin in urine using surface-enhanced Raman spectroscopy (SERS) and multivariate curve resolution on a nanostructured gold surface

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Abstract

A simple procedure is proposed for the determination of the antibiotic moxifloxacin in urine using nanostructured gold as surface-enhanced Raman scattering signal enhancer. The standard addition method in conjunction to multivariate curve resolution-alternating least squares was applied to eliminate the matrix effect and to isolate the spectral contribution of the analyte. Even in the presence of unexpected interferences in the urinary media, it was possible to extract and quantify the analyte response, reaching, in this way, the so-called second-order advantage from first-order data. Moreover, although a saturation phenomenon of the metallic surface was observed, the results of the proposed methodology presented important advantages such as high sensitivity and simpler experimental procedures. The moxifloxacin was determined at levels of 0.70 and 1.50 μg mL−1 in urine diluted to 1.0 % (corresponding to 70.0 and 150 μg mL−1 in the original samples) with relative errors of 4.23 and 8.70 %, respectively. The limit of detection (0.085 μg mL−1) and limit of quantification (0.26 μg mL−1) values indicated that the quantification can be accomplished in urine up to 24 h after the administration of a single 400-mg dose.

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Acknowledgments

The authors acknowledge the Instituto Nacional de Ciência e Tecnologia de Bioanalítica (INCTBio), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil, Brazil) for fellowships and financial support and to LME/LNNano/CNPEM for the technical support during the electron microscopy work.

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Authors and Affiliations

  1. Institute of Chemistry, State University of Campinas-UNICAMP, P.O. Box 6154, 13084-971, Campinas, SP, Brazil

    Mónica B. Mamián-López & Ronei J. Poppi

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  1. Mónica B. Mamián-López
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  2. Ronei J. Poppi
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Correspondence to Ronei J. Poppi.

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Published in the topical collection (Bio)Analytical Research in Latin America with guest editors Marco A. Zezzi Arruda and Lauro Kubota.

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Mamián-López, M.B., Poppi, R.J. Quantification of moxifloxacin in urine using surface-enhanced Raman spectroscopy (SERS) and multivariate curve resolution on a nanostructured gold surface. Anal Bioanal Chem 405, 7671–7677 (2013). https://doi.org/10.1007/s00216-013-7200-y

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  • DOI: https://doi.org/10.1007/s00216-013-7200-y

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