Abstract
Meconium is the earliest stool of newborns, and is a complex matrix that reflects the degree of exposure of the fetus to xenobiotics. To investigate fetal exposure to volatile organic compounds, an analytical method was developed to identify and quantify BTEX (benzene, toluene, ethylbenzene, and o,m,p-xylene) and two chlorinated solvents (trichloroethylene and tetrachloroethylene) in meconium. Headspace-solid-phase microextraction coupled with gas chromatography–mass spectrometry was selected because it is simple, sensitive, can be automated, and requires no extensive sample preparation. Several extraction variables were optimized (fiber type, incubation time, temperature of fiber, and use of salt). Because meconium is a complex matrix, quantification by SPME was considered carefully because of potential interference, for example competitive adsorption. Calibration in water was compared with calibration in meconium using external and internal methods (with isotope-labeled compounds). In meconium, limits of quantification were determined to be in the range 0.064–0.096 ng g−1 for the investigated compounds. All target compounds were determined in “real-case” meconium samples.
Similar content being viewed by others
References
Directive 2008/112/EC of the European Parliament and of the Council (2008) Off J Eur Union
Llop S, Ballester F, Estarlich M, Esplugues A, Rebagliato M, Iniguez C (2010) Environ Res 110:778–785
Lehmann I, Thoelke A, Rehwagen M, Rolle-Kampczyk U, Schlink U, Schulz R, Borte M, Diez U, Herbarth O (2002) Environ Toxicol 17:203–210
Lupo PJ, Symanski E, Waller DK, Chan W, Langlois PH, Canfield MA, Mitchell LE (2011) Environ Health Perspect 119:397–402
Garlantezec R, Multigner L, Labat L, Bonvallot N, Pulkkinen J, Dananche B, Monfort C, Rouget F, Cordier S (2012) Occup Environ Med 69:62–70
Whyatt RM, Camann D, Perera FP, Rauh VA, Tang D, Kinney PL, Garfinkel R, Andrews H, Hoepner L, Barr DB (2005) Toxicol Appl Pharmacol 206:246–254
Aguilera I, Guxens M, Garcia-Esteban R, Corbella T, Nieuwenhuijsen MJ, Foradada CM, Sunyer J (2009) Environ Health Perspect 117:1322–1327
Dowty BJ, Laseter JL, Storer J (1976) Pediatr Res 10:696–701
Bradman A, Barr DB, Henn BGC, Drumheller T, Curry C, Eskenazi B (2003) Environ Health Perspect 111:1779–1782
Barr DB, Wang RY, Needham LL (2005) Environ Health Perspect 113:1083–1091
Bearer CF (2003) Ambul Pediatr 3:40–43
Lozano J, Garcia-Algar O, Vall O, de la Torre R, Scaravelli G, Pichini S (2007) Ther Drug Monit 29:711–734
Ostrea EM (1999) Curr Probl Pediatr 29:37–56
Bar-Oz B, Klein J, Karaskov T, Koren G (2003) Arch Dis Child 88:F98–F100
Ostrea EM, Bielawski DM, Posecion NC, Corrion M, Villanueva-Uy E, Jin Y, Janisse JJ, Ager JW (2008) Environ Res 106:277–283
Ostrea EM, Parks PM, Brady MJ (1988) Clin Chem 34:2372–2373
Pichini S, Pacifici R, Pellegrini M, Marchei E, Lozano J, Murillo J, Vall O, Garcia-Algar O (2004) Anal Chem 76:2124–2132
Moore C, Negrusz A, Lewis D (1998) J Chromatogr B 713:137–146
Ostrea EM, Brady M, Gause S, Raymundo AL, Stevens M (1992) Pediatrics 89:107–113
Bearer CF, Lee S, Salvator AE, Minnes S, Swick A, Yamashita T, Singer LT (1999) Alcohol Clin Exp Res 23:487–493
Moore C, Jones J, Lewis D, Buchi K (2003) Clin Chem 49:133–136
Morini L, Marchei E, Vagnarelli F, Garcia-Algar O, Groppi A, Mastrobattista L, Pichini S (2010) Forensic Sci Int 196:74–77
Tsatsakis AM, Tzatzarakis MN, Koutroulakis D, Toutoudaki M, Sifakis S (2009) Xenobiotica 39:364–373
Ostrea EM, Morales V, Ngoumgna E, Prescilla R, Tan E, Hernandez E, Ramirez GB, Cifra HL, Manlapaz ML (2002) Neurotoxicology 23:329–339
Ostrea EM, Reyes A, Villanueva-Uy E, Pacifico R, Benitez B, Ramos E, Bernardo RC, Bielawski DM, Delaney-Black V, Chiodo L, Janisse JJ, Ager JW (2012) Neurotoxicology 33:669–675
Whyatt RM, Barr DB (2001) Environ Health Perspect 109:471–420
Dunemann L, Hajimiragha H (1993) Anal Chim Acta 283:199–206
Rosell M, Lacorte S, Ginebreda A, Barcelo D (2003) J Chromatogr A 995:171–184
Rosell M, Lacorte S, Barcelo D (2006) J Chromatogr A 1132:28–38
Johns DO, Dills RL, Morgan MS (2005) J Chromatogr B 817:255–261
Angerer J, Scherer G, Schaller KH, Muller J (1991) Fresenius J Anal Chem 339:740–742
Arambarri I, Lasa M, Garcia R, Millan E (2004) J Chromatogr A 1033:193–203
Ezquerro O, Ortiz G, Pons B, Tena MT (2004) J Chromatogr A 1035:17–22
Pawliszyn J (2009) Handbook of solid phase microextraction. Chemical Industry Press, Beijing
Ouyang G, Pawliszyn J (2006) Anal Bioanal Chem 386:1059–1073
Zhang ZY, Pawliszyn J (1993) J High Res Chromatog 16:689–692
Lestremau F, Andersson FAT, Desauziers V, Fanlo JL (2003) Anal Chem 75:2626–2632
Tuduri L, Desauziers V, Fanlo JL (2003) Analyst 128:1028–1032
Tang B, Isacsson U (2006) J Chromatogr A 1137:15–21
Mills GA, Walker V (2000) J Chromatogr A 902:267–287
Kusano M, Mendez E, Furton KG (2011) Anal Bioanal Chem 400:1817–1826
Garner CE, Ewer AK, Elasouad K, Power F, Greenwood R, Ratcliffe NM, Costello BDL, Probert CS (2009) J Pediatr Gastroenterol Nutr 49:559–565
Mills GA, Walker V, Mughal H (1999) J Chromatogr B 730:113–122
Macgillivray B, Pawliszyn J, Fowlie P, Sagara C (1994) J Chromatogr Sci 32:317–322
Murat C, Gourrat K, Jerosch H, Cayot N (2012) Food Chem 135:913–920
Hutson JR, Aleksa K, Pragst F, Koren G (2009) J Chromatogr B 877:8–12
Hutson JR, Rao C, Fulga N, Aleksa K, Koren G (2011) Alcohol 45:193–199
Bakdash A, Burger P, Goecke TW, Fasching PA, Reulbach U, Bleich S, Hastedt M, Rothe M, Beckmann MW, Pragst F, Kornhuber J (2010) Anal Bioanal Chem 396:2469–2477
Roehsig M, de Paula DM, Moura S, Diniz EM, Yonamine M (2010) J Sep Sci 33:2115–2122
Arthur CL, Pawliszyn J (1990) Anal Chem 62:2145–2148
Arthur CL, Killam LM, Buchholz KD, Pawliszyn J, Berg JR (1992) Anal Chem 64:1960–1966
Zhang ZY, Pawliszyn J (1993) Anal Chem 65:1843–1852
Lord H, Pawliszyn J (2000) J Chromatogr A 885:153–193
Zygmunt B, Jastrzebska A, Namiesnik J (2001) Crit Rev Anal Chem 31:1–18
SPME–adsorption versus absorption: which fiber is best for your application? (1999) Supelco, Bellefonte
Lee MR, Chang CM, Dou J (2007) Chemosphere 69:1381–1387
Gorecki T, Yu XM, Pawliszyn J (1999) Analyst 124:643–649
Fustinoni S, Giampiccolo R, Pulvirenti S, Buratti M, Colombi A (1999) J Chromatogr B 723:105–115
Pawliszyn J (2009) Handbook of solid phase microextraction. Chemical Industry Press, Beijing
Vichi S, Pizzale L, Conte LS, Buxaderas S, Lopez-Tamames E (2005) J Chromatogr A 1090:146–154
Lestremau F, Desauziers V, Fanlo JL (2004) Anal Bioanal Chem 378:190–196
Khaled A, Pawliszyn J (2000) J Chromatogr A 892:455–467
Lestremau F, Desauziers V, Roux JC, Fanlo JL (2003) J Chromatogr A 999:71–80
Lord H, Pawliszyn J (2000) J Chromatogr A 902:17–63
Acknowledgments
The authors thank Dr Loget of the anatomical pathology service of Rennes University Hospital for providing meconium samples. This work was supported by the national Programme Hospitalier de Recherche Clinique (PHRC) 2010 accorded to Rennes University Hospital, and was performed in collaboration with the Brittany Registry of Congenital Malformations, the INSERM-IRSET (Institut de Recherche en Santé, Environnement et Travail) Unities 1085 and 991, the Centre de Ressources Biologiques (CRB) of the Centre d’Investigations Cliniques (CIC) of Rennes University Hospital.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(PDF 374 kb)
Rights and permissions
About this article
Cite this article
Meyer-Monath, M., Beaumont, J., Morel, I. et al. Analysis of BTEX and chlorinated solvents in meconium by headspace-solid-phase microextraction gas chromatography coupled with mass spectrometry. Anal Bioanal Chem 406, 4481–4490 (2014). https://doi.org/10.1007/s00216-014-7836-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-014-7836-2