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Approaches for the detection of harmful algal blooms using oligonucleotide interactions

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Abstract

Blooms of microscopic algae in our waterways are becoming an increasingly important environmental concern. Many are sources of harmful biotoxins that can lead to death in humans, marine life and birds. Additionally, their biomass can cause damage to ecosystems such as oxygen depletion, displacement of species and habitat alteration. Globally, the number and frequency of harmful algal blooms has increased over the last few decades, and monitoring and detection strategies have become essential for managing these events. This review discusses developments in the use of oligonucleotide-based ‘molecular probes’ for the selective monitoring of algal cell numbers. Specifically, hybridisation techniques will be a focus.

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Authors and Affiliations

  1. School of Chemical and Physical Sciences, Flinders University, Sturt Road, Bedford Park, Adelaide, SA, 5042, Australia

    Karen L. Bruce & Claire E. Lenehan

  2. School of Biological Sciences, Flinders University, Sturt Road, Bedford Park, Adelaide, SA, 5042, Australia

    Sophie C. Leterme

  3. Flinders Centre for Nanoscale Science and Technology, Flinders University, Sturt Road, Bedford Park, Adelaide, SA, 5042, Australia

    Amanda V. Ellis

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  2. Sophie C. Leterme
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  3. Amanda V. Ellis
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  4. Claire E. Lenehan
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Correspondence to Claire E. Lenehan.

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Bruce, K.L., Leterme, S.C., Ellis, A.V. et al. Approaches for the detection of harmful algal blooms using oligonucleotide interactions. Anal Bioanal Chem 407, 95–116 (2015). https://doi.org/10.1007/s00216-014-8193-x

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  • DOI: https://doi.org/10.1007/s00216-014-8193-x

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