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Macromolecular crowding-assisted fabrication of liquid-crystalline imprinted polymers

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Abstract

A macromolecular crowding-assisted liquid-crystalline molecularly imprinted monolith (LC-MIM) was prepared successfully for the first time. The imprinted stationary phase was synthesized with polymethyl methacrylate (PMMA) or polystyrene (PS) as the crowding agent, 4-cyanophenyl dicyclohexyl propylene (CPCE) as the liquid-crystal monomer, and hydroquinidine as the pseudo-template for the chiral separation of cinchona alkaloids in HPLC. A low level of cross-linker (26 %) has been found to be sufficient to achieve molecular recognition on the crowding-assisted LC-MIM due to the physical cross-linking of mesogenic groups in place of chemical cross-linking, and baseline separation of quinidine and quinine could be achieved with good resolution (R s = 2.96), selectivity factor (α = 2.16), and column efficiency (N = 2650 plates/m). In contrast, the LC-MIM prepared without crowding agents displayed the smallest diastereoselectivity (α = 1.90), while the crowding-assisted MIM with high level of cross-linker (80 %) obtained the greatest selectivity factor (α = 7.65), but the lowest column efficiency (N = 177 plates/m).

Schematic representation of crowding-assisted liquid crystalline imprinted polymer

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 21375096 and U1303202).

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Authors and Affiliations

  1. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China

    Chen Zhang, Jing Zhang, Yan-Ping Huang & Zhao-Sheng Liu

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  1. Chen Zhang
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  2. Jing Zhang
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  3. Yan-Ping Huang
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  4. Zhao-Sheng Liu
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Correspondence to Yan-Ping Huang or Zhao-Sheng Liu.

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Zhang, C., Zhang, J., Huang, YP. et al. Macromolecular crowding-assisted fabrication of liquid-crystalline imprinted polymers. Anal Bioanal Chem 407, 2923–2931 (2015). https://doi.org/10.1007/s00216-015-8510-z

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  • DOI: https://doi.org/10.1007/s00216-015-8510-z

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