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Determination of eugenol in fish and shrimp muscle tissue by stable isotope dilution assay and solid-phase extraction coupled gas chromatography–triple quadrupole mass spectrometry

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Abstract

In this study, we developed a new method for the accurate quantification of eugenol in fish samples based on stable isotope dilution assay (SIDA) and solid-phase extraction (SPE) coupled gas chromatography–triple quadrupole mass spectrometry (SIDA-SPE-GC-MS/MS). Due to the difference of matrix effect (ME), it was difficult to determine accurately the level of eugenol residue in different fish and shrimp samples based on external standard calibration method. SIDA was applied to compensate matrix effect (ME) that eugenol-d3 was used as internal standard (IS). Freshwater fish (carp, channel catfish), marine fish (turbot), and shrimp (Penaeus vannawei) were used for the method validation. The average recoveries of eugenol were in the range of 94.7 to 109.78 % when the spiking levels were 10, 50, and 200 μg kg−1. The inter-day and intra-day precisions were in the range of 1.15–8.19 and 0.71–8.45 %. The limit of detection (LOD) and the limit of quantification (LOQ) were approximately 2.5 and 5.0 μg kg−1. This method was applied to the real fish samples assay obtained from aquaculture markets in Beijing, China. Eugenol residue was found in two fish samples with the levels at 6.2 and 7.7 μg kg−1, respectively.

Determination of eugenol in fish and shrimp muscle tissue

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Acknowledgments

This research was supported by the Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2016ZD1104).

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Authors and Affiliations

  1. Chinese Academy of Fishery Sciences, No. 150 Qingta, Yongding Road, Beijing, 100141, China

    Jincheng Li, Huan Liu, Chaoying Wang & Lidong Wu

  2. Beijing University of Agriculture, Beinong Road 7, Huilongguan, Changping District, Beijing, 102206, China

    Dan Liu

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  1. Jincheng Li
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  2. Huan Liu
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  3. Chaoying Wang
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  4. Lidong Wu
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  5. Dan Liu
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Correspondence to Jincheng Li.

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All animal procedures were approved and conducted under the guidelines of the Chinese Academy of Fishery Sciences (Beijing, China).

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The authors declare that they have no competing interests.

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Li, J., Liu, H., Wang, C. et al. Determination of eugenol in fish and shrimp muscle tissue by stable isotope dilution assay and solid-phase extraction coupled gas chromatography–triple quadrupole mass spectrometry. Anal Bioanal Chem 408, 6537–6544 (2016). https://doi.org/10.1007/s00216-016-9850-z

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  • DOI: https://doi.org/10.1007/s00216-016-9850-z

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