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Development of Stable Isotope Dilution Quantification Liquid Chromatography–Mass Spectrometry Method for Estimation of Exposure Levels of Bisphenol A, 4-tert-Octylphenol, 4-Nonylphenol, Tetrabromobisphenol A, and Pentachlorophenol in Indoor Air

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Abstract

The potential risks to human health and reproduction of phenolic xenoestrogens, such as bisphenol A (BPA), 4-tert-octylphenol (OP), 4-nonylphenol (NP), tetrabromobisphenol A (TBBPA), and pentachlorophenol (PCP), have not been fully elucidated. It is possible that healthy humans are exposed to phenolic xenoestrogens in their daily lives. It is also possible that the source of human exposure to trace amounts of phenolic xenoestrogens is indoor air. Therefore, we have established an accurate, sensitive, and selective method for determining BPA, OP, NP, TBBPA, and PCP in air samples by using stable isotope dilution techniques using 13C12-BPA, 4-(1-methyl) octylphenol-d5 (m-OP-d5), 13C12-TBBPA, 13C6-PCP, and liquid chromatography–mass spectrometry (LC-MS). Air sampling (71/min, 24 hours), using glass filters (GB-100R, 47 mm) and Empore SDB-XD filters (47 mm/0.5 mm, 3M, Tokyo, Japan) and subsequent extraction by washing were carried out. Then the extracted sample solution was subjected to LC-MS. The percentage recovery of these analytes ranged from 87.0% to 101.9% (SD 0.2% to 4.6%, n = 3) with correction using the stable isotope dilution quantification method. The quantification limit (signal-to-noise ratio, S/N>10) in the air samples was 0.1 ng/m3. Our findings suggest that the LC-MS method can detect low levels of phenolic xenoestrogens in air samples and that these trace levels of analytes exist in indoor air.

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References

  • Brock JW, Yoshimura Y, Barr JR, Maggio VL, Sam R, Nakazawa H, et al. (2001) Measurement of bisphenol A levels in human urine. J Expo Anal Environ Epidemiol 11:323–328

    Article  CAS  Google Scholar 

  • Calafat AM, Kuklenyik Z, Reidy JA, Caudill SP, Ekong J, Needham LL (2005) Urinary concentrations of bisphenol A and 4-nonylphenol in a human reference population. Environ Health Perspect 113:391–395

    Article  CAS  Google Scholar 

  • Damstra T, Barlow S, Bergman A, Kavlock R, Kraak GVD. International programme on chemical safety: Global assessment of the state-of-the-science of endocrine disruptors. Available at: http://www.ehp.niehs.nih.gov/who/ . Accessed: 1 July 2006.

  • Guenther K, Heinke V, Thiele B, Kleist E, Prast H, Raecker T (2003) Endocrine disrupting nonylphenols are ubiquitous in food. Environ Sci Technol 36:1676–1680

    Article  CAS  Google Scholar 

  • Hirayama K, Tanaka H, Kawana K, Tani T, Nakazawa H (2001) Analysis of plasticizers in cap-sealing resins for bottled foods. Food Addit Contam 18:357–362

    Article  CAS  Google Scholar 

  • Horie M, Yoshida T, Ishii R, Kobayashi S, Nakazawa H (1999) Determination of bisphenol A in canned drinks by LC/MS. Bunseki Kagaku 48:579–588

    CAS  Google Scholar 

  • Inoue K, Yoshimura Y, Makino T, Nakazawa H (2000a) Determination of 4-nonylphenol and 4-octylphenol in human blood samples by high-performance liquid chromatography with multi-electrode electrochemical coulometric-array detection. Analyst 125:1959–1961

    Article  CAS  Google Scholar 

  • Inoue K, Kato K, Yoshimura Y, Makino T, Nakazawa H (2000b) Determination of bisphenol A in human serum by high-performance liquid chromatography with multi-electrode electrochemical detection. J Chromatogr B 749:17–23

    CAS  Google Scholar 

  • Inoue K, Kondo S, Yoshie Y, Kato K, Yoshimura Y, Horie M, et al (2001a) Migration of 4-nonylphenol from polyvinyl chloride food packaging films into food simulants and foods. Food Addit Contam 18:157–164

    Article  CAS  Google Scholar 

  • Inoue K, Yamaguchi A, Wada M, Yoshimura Y, Makino T, Nakazawa H (2001b) Quantitative detection of bisphenol A and bisphenol A diglycidyl ether metabolites in human plasma by liquid chromatography–electrospray mass spectrometry. J Chromatogr B 765(2):121–126

    CAS  Google Scholar 

  • Inoue K, Kobayashi N, Yoshimura Y, Horie M, Nakazawa H (2002a) Estimation of estrogenic activity of polyvinyl chloride films for food wrapping by estrogen receptor binding assay. Jpn J Food Chem 9:46–49

    CAS  Google Scholar 

  • Inoue K, Okumura H, Higuchi T, Oka H, Yoshimura Y, Nakazawa H (2002b) Characterization of estrogenic compounds in medical polyvinyl chloride tubing by gas chromatography–mass spectrometry and estrogen receptor binding assay. Clin Chim Acta 325:157–163

    Article  CAS  Google Scholar 

  • Inoue K, Yoshie Y, Kondo S, Yoshimura Y, Nakazawa H (2002c) Determination of phenolic xenoestrogens in water by liquid chromatography with coulometric-array detection. J Chromatogr A 946:291–294

    Article  CAS  Google Scholar 

  • Inoue K, Wada M, Higuchi T, Oshio S, Umeda T, Yoshimura Y, et al. (2002d) Application of liquid chromatography–mass spectrometry to the quantification of bisphenol A in human semen. J Chromatogr B 773:97–102

    Google Scholar 

  • Inoue K, Murayama S, Takeba K, Yoshimura Y, Nakazawa H (2003a) Contamination of xenoestrogens bisphenol A and F in honey: Safety assessment and analytical method of these compounds in honey. J Food Compost Anal 16:497–506

    Article  CAS  Google Scholar 

  • Inoue K, Kawaguchi M, Okada F, Takai N, Yoshimura Y, Horie M, et al. (2003b) Measurement of 4-nonylphenol and 4-tert-octylphenol in human urine by column-switching liquid chromatography–mass spectrometry. Anal Chim Acta 486:41–50

    Article  CAS  Google Scholar 

  • Inoue K, Kawaguchi M, Funakoshi Y, Nakazawa H (2003c) Size-exclusion flow extraction of bisphenol A in human urine for liquid chromatography–mass spectrometry. J Chromatogr B 798:17–23

    Article  CAS  Google Scholar 

  • Jeannot R, Sabik H, Sauvard E, Dagnac T, Dohrendorf K (2002) Determination of endocrine-disrupting compounds in environmental samples using gas and liquid chromatography with mass spectrometry. J Chromatogr A 974:143–159

    Article  CAS  Google Scholar 

  • Kamuura T, Tajima Y, Nakahara T (1997) Determination of bisphenol A in air by GC/MS. J Environ Chem Jpn 7:275–280

    Google Scholar 

  • Kawaguchi M, Inoue K, Sakui N, Ito R, Izumi S, Makino T, et al. (2004) Stir bar sorptive extraction and thermal desorption–gas chromatography–mass spectrometry for the measurement of 4-nonylphenol and 4-tert-octylphenol in human biological samples. J Chromatogr B 799:119–125

    Article  CAS  Google Scholar 

  • Kuroda N, Kinoshita Y, Sun Y, Wada M, Kishikawa N, Nakashima K, et al. (2003) Measurement of bisphenol A levels in human blood serum and ascitic fluid by HPLC using a fluorescent labeling reagent. J Pharm Biomed Anal 30:1743–1749

    Article  CAS  Google Scholar 

  • Loos R, Hanke G, Eisenreich SJ (2003) Multi-component analysis of polar water pollutants using sequential solid-phase extraction followed by LC-ESI-MS. J Environ Monit 5:384–394

    Article  CAS  Google Scholar 

  • Müller S, Schmid P, Schlatter C (1998) Pharmacokinetic behavior of 4-nonylphenol in humans. Environ Toxicol Pharm 5:257–265

    Article  Google Scholar 

  • Peltonen K, Pukkila J (1988) Determination of bisphenol A in air by high-performance liquid chromatography with electrochemical detection. J Chromatogr 439:375–380

    Article  CAS  Google Scholar 

  • Petrovic M, Eljarrat E, Lopez de Alda MJ, Barcelo D (2002a) Recent advances in the mass spectrometric analysis related to endocrine disrupting compounds in aquatic environmental samples. J Chromatogr A 974:23–51

    Article  CAS  Google Scholar 

  • Petrovic M, Tavazzi S, Barcelo D (2002b) Column-switching system with restricted access pre-column packing for an integrated sample cleanup and liquid chromatographic–mass spectrometric analysis of alkylphenolic compounds and steroid sex hormones in sediment. J Chromatogr A 971:37–45

    Article  CAS  Google Scholar 

  • Rudel RA, Brody JG, Spengler JD, Vallarino J, Geno PW, Sun G, et al. (2001) Identification of selected hormonally active agents and animal mammary carcinogens in commercial and residential air and dust samples. J Air Waste Manag Assoc 51:499–513

    CAS  Google Scholar 

  • Rudel RA, Camann DE, Spengler JD, Korn LR, Brody JG (2003) Phthalates, alkylphenols, pesticides, polybrominated diphenyl ethers, and other endocrine-disrupting compounds in indoor air and dust. Environ Sci Technol 37:4543–4553

    Article  CAS  Google Scholar 

  • Sun Y, Wada M, Al-Dirbashi O, Kuroda N, Nakazawa H, Nakashima K (2000) High-performance liquid chromatography with peroxyoxalate chemiluminescence detection of bisphenol A migrated from polycarbonate baby bottles using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a label. J Chromatogr B 749:49–56

    CAS  Google Scholar 

  • The Environment Agency of Japan, SPEED ’98/JEA (1998) Strategic programs on environmental endocrine disrupters ’98. Available at: http://www.env.go.jp/en/pol/speed98/sp98.html . Accessed: 1 July 2006

  • The Ministry of Health, Labour and Welfare of Japan. Information of endocrine disruptors, Japanese. Available at: http://www.nihs.go.jp/edc/edc.html . Accessed: 1 July 2006

  • Yoshida T, Horie M, Hoshino Y, Nakazawa H (2001) Determination of bisphenol A in canned vegetables and fruit by high performance liquid chromatography. Food Addit Contam 18:69–75

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Analytic Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan

    K. Inoue, S. Yoshida, R. Ito, N. Okanouchi & H. Nakazawa

  2. Department of Environmental Science, Japan Environmental Sanitation Center, Kawasaki City, Kanagawa, Japan

    S. Nakayama

Authors
  1. K. Inoue
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  2. S. Yoshida
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  3. S. Nakayama
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  4. R. Ito
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  5. N. Okanouchi
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  6. H. Nakazawa
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Corresponding author

Correspondence to H. Nakazawa.

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Inoue, K., Yoshida, S., Nakayama, S. et al. Development of Stable Isotope Dilution Quantification Liquid Chromatography–Mass Spectrometry Method for Estimation of Exposure Levels of Bisphenol A, 4-tert-Octylphenol, 4-Nonylphenol, Tetrabromobisphenol A, and Pentachlorophenol in Indoor Air. Arch Environ Contam Toxicol 51, 503–508 (2006). https://doi.org/10.1007/s00244-005-0236-z

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  • DOI: https://doi.org/10.1007/s00244-005-0236-z

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