Skip to main content

Advertisement

SpringerLink
Log in

Comparison of Toxicities of Metal Pyrithiones Including Their Degradation Compounds and Organotin Antifouling Biocides to the Japanese Killifish Oryzias latipes

  • Special Issue: Indicators of Ocean Pollution
  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Japanese killifish Oryzias latipes were exposed to three levels (0, 1, and 10 µg l−1) of copper pyrithione (CuPT2), zinc pyrithione (ZnPT2), six of their degradation products, and the organotin compounds tributyltin (TBT) and triphenyltin (TPT) for 48 h at 20 °C. All individual fish exposed to 1 and 10 µg l−1 of CuPT2 or 10 µg l−1 of ZnPT2 were dead within 12 h, respectively, and at 24 h the survival rate of the fish exposed to 1 µg l−1 of ZnPT2 was 50%. All fish exposed to 10 µg l−1 of ZnPT2 showed morphological abnormalities in the form of vertebral deformity. None of the fish exposed to six of the degradation products of PTs, TBT, and TPT died during a 48-h exposure period, but various biological effects were observed in the fish exposed to these chemicals: abnormalities of respiration and swimming behavior, and decreased hatchability. Our findings suggest that O. latipes has a higher ecological risk of CuPT2 and ZnPT2 exposure than of TBT and TPT exposure during their life history. Because these antifouling biocides have been used in both freshwater and marine environments, our results highlight these biocides’ deleterious effects on the freshwater fish as well as marine fish, and they indicate freshwater and marine pollution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Armbrust KL (2000) Pesticide hydroxyl radical rate constants: measurements and estimates of their importance in aquatic environments. Environ Toxicol Chem 19:2175–2180

    Article  CAS  Google Scholar 

  • Bellas J, Granmo Ã…, Beiras R (2005) Embryotoxicity of the antifouling biocide zinc pyrithione to sea urchin (Paracentrotus lividus) and mussel (Mytilus edulis). Mar Pollut Bull 50:1382–1385

    Article  CAS  Google Scholar 

  • Cho HS, Lam NH, Lee JS, Seol SW (2014) Organotins pollutions in seawater and sediment around a shipyard. Toxicol Environ Health Sci 6:33–40

    Article  Google Scholar 

  • Colton MD, Kwok KWH, Brandon JA, Warren IH, Ryde IT, Cooper EM, Hinton DE, Rittschof D, Meyer JN (2014) Developmental toxicity and DAN damage from exposure to parking lot runoff retention pond samples in the Japanese Medaka (Oryzias latipes). Mar Environ Res 99:117–124

    Article  CAS  Google Scholar 

  • Dong S, Kang M, Wu X, Ye T (2014) Development of a promising fish model (Oryzias melastigma) for assessing multiple responses to stresses in the marine environment. BioMed Res Int 2014:1–17

    Google Scholar 

  • Eriksson Wiklund AK, Börjesson T, Wiklund SJ (2006) Avoidance response of sediment living amphipods to zinc pyrithione as a measure of sediment toxicity. Mar Pollut Bull 52:96–99

    Article  CAS  Google Scholar 

  • Evans DH (1987) The fish gill: site of action and model for toxic effects of environmental pollutants. Environ Health Perspect 71:47–58

    Article  CAS  Google Scholar 

  • Gerhardt A, Ingram MK, Kang IJ, Ulitzur S (2006) In situ on-line biomonitoring in water: recent developments. Environ Toxicol Chem 25:2263–2271

    Article  CAS  Google Scholar 

  • Gibson WB, Jeffcoat AR, Turan TS, Wendt RH, Hughes PF, Twine ME (1982) Zinc pyridinethione: serum metabolites of zinc pyridinethione in rabbits, rats, monkeys, and dogs after oral dosing. Toxicol Appl Pharmacol 62:237–250

    Article  CAS  Google Scholar 

  • Girard JP, Szpunar J, Pedrotti ML, Pesando D (2000) Toxicity of tri-n-butyltin to sea urchin eggs and larvae: relation to bioaccumulation at the nanomolar level. Environ Toxicol Chem 19:1272–1277

    Article  CAS  Google Scholar 

  • Goka K (1999) Embryotoxicity of zinc pyrithione, an antidandruff chemical, in fish. Environ Res Sect A 81:81–83

    Article  CAS  Google Scholar 

  • Gormley KL, Teather KL (2003) Developmental, behavioral, and reproductive effects experienced by Japanese medaka (Oryzias latipes) in response to short-term exposure to endosulfan. Ecotoxicol Environ Saf 54:330–338

    Article  CAS  Google Scholar 

  • Grillitsch B, Vogl C, Wytek R (1999) Qualification of spontaneous undirected locomotor behavior of fish for sublethal toxicity testing. Part II. Variability of measurement parameters under toxicant-induced stress. Environ Toxicol Chem 18:2743–2750

    Article  CAS  Google Scholar 

  • Harino H, Mori Y, Yamaguchi Y, Shibata K, Senda T (2005) Monitoring of antifouling booster biocides in water and sediment from the Port of Osaka, Japan. Arch Environ Contam Toxicol 48:303–310

    Article  CAS  Google Scholar 

  • Harino H, Midorikawa S, Arai T, Ohji M, Cu ND, Miyazaki N (2006) Concentrations of booster biocides in sediment and clams from Vietnam. Mar Biol Assoc UK 86:1163–1170

    Article  CAS  Google Scholar 

  • Heath AG, Cech JJ Jr, Brink L, Moberg P, Zink G (1997) Physiological responses of fathead minnow larvae to rice pesticides. Ecotoxicol Environ Saf 37:280–288

    Article  CAS  Google Scholar 

  • Hofer R, Jeney Z, Bucher F (1995) Chronic effects of linear alkylbenzene sulfonate (LAS) and ammonia on rainbow trout (Oncorhynchus mykiss) fry at water criteria limits. Water Res 29:2725–2729

    Article  CAS  Google Scholar 

  • Johansen K, Møhlenberg F (1987) Impairment of egg production in Acartia tonsa exposed to tributyltin oxide. Ophelia 27:137–141

    Article  Google Scholar 

  • Karlsson J, Eklund B (2004) New biocide-free anti-fouling paints are toxic. Mar Pollut Bull 49:456–464

    Article  CAS  Google Scholar 

  • Kim NS, Shim WJ, Yim UH, Hong SH, Ha SY, Han GM, Shin KH (2014) Assessment of TBT and organic booster biocide contamination in seawater from coastal areas of South Korea. Mar Pollut Bull 78:201–208

    Article  CAS  Google Scholar 

  • Koutoku T, Zhang R, Tachibana T, Oshima Y, Furuse M (2003) Effect of acute l-tryptophan exposure on the brain serotonergic system and behavior in the male medaka. Zool Sci 20:121–124

    Article  CAS  Google Scholar 

  • Krümmel EM, MacDonald RW, Kimpe LE, Gregory-Eaves I, Demers MJ, Smol JP, Finney B, Blais JM (2003) Delivery of pollutants by spawning salmon. Nature 425:255–256

    Article  Google Scholar 

  • Luckenbach T, Triebskorn R, Müller E, Oberemm A (2001) Toxicity of waters from two streams to early life stages of brown trout (Salmo trutta f. fario L.), tested under semi-field conditions. Chemosphere 45:571–579

    Article  CAS  Google Scholar 

  • Madsen T, Samsøe-Petersen L, Gustavson K, Rasmussen D (2000) Ecotoxicological assessment of antifouling biocides and nonbiocidal antifouling paints. Environmental project report 531. Danish Environmental Protection Agency, Copenhagen, Denmark

  • Maraldo K, Dahllöf I (2004) Indirect estimation of degradation time for zinc pyrithione and copper pyrithione in seawater. Mar Pollut Bull 48:894–901

    Article  CAS  Google Scholar 

  • Maria AS, Pozuelo JM, Lopez A, Sanz F (1996) Toxicity of potential irritants in mammalian cells in vitro. Ecotoxicol Environ Saf 34:56–58

    Article  Google Scholar 

  • Mochida K, Ito K, Harino H, Kakuno A, Fujii K (2006) Acute toxicity of antifouling biocides and joint toxicity with copper to red sea bream (Pagrus major) and toy shrimp (Heptacarpus futilirostris). Environ Toxicol Chem 25:3058–3064

    Article  Google Scholar 

  • Mochida K, Ito K, Harino H, Onduka T, Kakuno A, Fujii K (2008) Early life-stage toxicity test for copper pyrithione and induction of skeletal anomaly in a teleost, the mummichog (Fundulus heteroclitus). Environ Toxicol Chem 27:367–374

    Article  CAS  Google Scholar 

  • Mochida K, Ito K, Harino H, Tanaka H, Onduka T, Kakuno A, Fujii K (2009) Inhibition of acetylcholinesterase by metabolites of copper pyrithione (CuPT) and its possible involvement in vertebral deformity of a CuPT-exposed marine teleostean fish. Comp Biochem Physiol C 149:624–630

    Google Scholar 

  • Nakajima K, Yasuda T (1990) High-performance liquid chromatographic determination of zinc pyrithione in antidandruff preparations based on copper chelate formation. J Chromatogr 502:379–384

    Article  CAS  Google Scholar 

  • Nakayama K, Oshima Y (2008) Adverse effects of tributyltin on reproduction of Japanese medaka, Oryzias latipes. Coast Mar Sci 32:67–76

    Google Scholar 

  • Nakayama K, Oshima Y, Tachibana T, Furuse M, Honjo T (2007) Alternation of monoamine concentrations in the brain of medaka, Oryzias latipes. Environ Toxicol 22:53–57

    Article  CAS  Google Scholar 

  • Ohji M (2009) Biological effects of tributyltin on the Caprellidea (Crustacea: Amphipoda). In: Arai T, Harino H, Ohji M, Langston WJ (eds) Ecotoxicology of antifouling biocides. Springer, New York, pp 161–193

    Chapter  Google Scholar 

  • Ohji M, Arai T, Miyazaki N (2002) Effects of tributyltin exposure in the embryonic stage on sex ratio and survival rate in the caprellid amphipod Caprella danilevskii. Mar Ecol Prog Ser 235:171–176

    Article  CAS  Google Scholar 

  • Ohji M, Arai T, Miyazaki N (2007) Comparison of organotin accumulation in the masu salmon Oncorhynchus masou accompanying migratory histories. Estuar Coast Shelf Sci 72:721–731

    Article  CAS  Google Scholar 

  • Organization for Economic Cooperation and Development (1992) Fish, acute toxicity test (adopted 17 July 1992). Guideline 203. Paris, France, pp 1–9

  • Ososkov I, Weis JS (1996) Development of social behavior in larval mummichogs after embryonic exposure to methylmercury. Trans Am Fish Soc 125:983–987

    Article  CAS  Google Scholar 

  • Pfeifer K, Weber L (1979) The effect of carbon tetrachloride on the total plasma protein concentration of rainbow trout. Comp Biochem Physiol C 64:37–42

    Article  Google Scholar 

  • Rumampuk NDC, Grevo GS, Rumengan IFM, Ohji M, Arai T, Miyazaki N (2004) Effects of triphenyltin exposure on the red alga Eucheuma denticulatum. Coast Mar Sci 29:81–84

    Google Scholar 

  • Sakkas VA, Shibata K, Yamaguchi Y, Sugaswa S, Albanis T (2007) Aqueous phototransformation of zinc pyrithione degradation kinetics and byproduct identification by liquid chromatography—atmospheric pressure chemical ionization mass spectrometry. J Chromatogr A 1144:175–182

    Article  CAS  Google Scholar 

  • Sancho E, Ferrando MD, Andreu E (1997) Sublethal effects of an organophosphate insecticide on the European eel, Anguilla anguilla. Ecotoxicol Environ Saf 36:57–65

    Article  CAS  Google Scholar 

  • Triebskorn R, Kohler HR, Flemming J, Braunbeck T, Negele RD, Rahmann H (1994) Evaluation of bis (tri-n-butyltin) oxide (TBTO) neurotoxicity in rainbow trout (Oncorhynchus mykiss). I. Behaviour, weight increase, and tin content. Aquat Toxicol 30:189–197

    Article  CAS  Google Scholar 

  • Turley PA, Fenn RJ, Ritter JC (2000) Pyrithiones as antifoulants: environmental chemistry and preliminary risk assessment. Biofouling 15:175–182

    Article  CAS  Google Scholar 

  • Wang DY, Huang BQ (1998) Chronic effects of tributyltin on the locomotor of juvenile thornfish (Terapon jabua ForsskÃ¥i). J Fish Soc Taiwan 25:281–288

    CAS  Google Scholar 

  • Yamada H, Kakuno A (2002) Present status of the development of alternative tributyltin-free antifouling paints and toxicity of new biocides to aquatic organisms. Bull Fish Res Agency 6:56–72

    Google Scholar 

  • Yamamoto T (1975) Stage in development. In: Medaka (Killifish): biology and strains. Yugaku-sha, Tokyo, pp 30–63

Download references

Acknowledgements

The authors thank the staff of Kobe College for their sample collection and laboratory experiment support. This study was partially supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (Nos. 19681005, 22651008, and 26701008).

Author information

Authors and Affiliations

  1. Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Madoka Ohji

  2. School of Human Sciences, Kobe College, 4-1 Okadayama, Nishinomiya, Hyogo, Iwate, 662-8505, Japan

    Hiroya Harino

Authors
  1. Madoka Ohji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroya Harino
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Madoka Ohji.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ohji, M., Harino, H. Comparison of Toxicities of Metal Pyrithiones Including Their Degradation Compounds and Organotin Antifouling Biocides to the Japanese Killifish Oryzias latipes . Arch Environ Contam Toxicol 73, 285–293 (2017). https://doi.org/10.1007/s00244-017-0367-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00244-017-0367-z

Keywords

Search

Navigation