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Fluorescence photobleaching analysis for the study of cellular dynamics

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Abstract

The wide availability of the confocal microscope and the emergence of green fluorescent protein (GFP) transfection technology has led to the increasing use of photobleaching studies to examine aspects of cellular dynamics in living cells. In this review, we examine the theory and practice of performing photobleaching studies using a confocal microscope. We illustrate the application of photobleaching protocols using our own measurements of fluorescently labelled red blood cells and of malaria parasite-infected erythrocytes expressing GFP fusions and examine other examples from the literature.

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Authors and Affiliations

  1. Department of Biochemistry, La Trobe University, Bundoora, 3083 Victoria, Australia, Australia

    Nectarios Klonis, Melanie Rug & Leann Tilley

  2. Department of Biological Sciences, Monash University, Clayton, 3800 Victoria, Australia, Australia

    Ian Harper

  3. Infection and Immunity, Walter and Eliza Hall Institute of Medical Research, PO RMH, 3052 Victoria, Australia, Australia

    Mark Wickham & Alan Cowman

Authors
  1. Nectarios Klonis
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  2. Melanie Rug
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  3. Ian Harper
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  4. Mark Wickham
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  5. Alan Cowman
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  6. Leann Tilley
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Klonis, N., Rug, M., Harper, I. et al. Fluorescence photobleaching analysis for the study of cellular dynamics. Eur Biophys J 31, 36–51 (2002). https://doi.org/10.1007/s00249-001-0202-2

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  • DOI: https://doi.org/10.1007/s00249-001-0202-2

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