Skip to main content
SpringerLink
Log in

Cloning and characterization of two α-glucosidases from Bifidobacterium adolescentis DSM20083

  • Original Paper
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract.

Two α-glucosidase encoding genes (aglA and aglB) from Bifidobacterium adolescentis DSM 20083 were isolated and characterized. Both α-glucosidases belong to family 13 of the glycosyl hydrolases. Recombinant AglA (EC 3.2.1.10) and AglB (EC 3.2.1.20), expressed in Escherichia coli, showed high hydrolytic activity towards isomaltose and pnp-α-glucoside. The K m for pnp-α-glucoside was 1.05 and 0.47 mM and the V max was 228 and 113 U mg–1 for AglA and AglB, respectively. Using pnp-α-glucoside as substrate, the pH optimum for AglA was 6.6 and the temperature optimum was 37°C. For AglB, values of pH 6.8 and 47°C were found. AglA also showed high hydrolytic activity towards isomaltotriose and, to a lesser extent, towards trehalose. AglB has a high preference for maltose and less activity towards sucrose; minor activity was observed towards melizitose, low molecular weight dextrin, maltitol, and maltotriose. The recombinant α-glucosidases were tested for their transglucosylation activity. AglA was able to synthesize oligosaccharides from trehalose and sucrose. AglB formed oligosaccharides from sucrose, maltose, and melizitose.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Laboratory of Food Chemistry, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands, , , , ,

    L. van den Broek,  K. Struijs,  G. Beldman &  A. Voragen

  2. Laboratory of Industrial Microbiology, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands, , , , ,

    J. Verdoes

Authors
  1. L. van den Broek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Struijs
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Verdoes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Beldman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Voragen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Cite this article

van den Broek, .L., Struijs, .K., Verdoes, .J. et al. Cloning and characterization of two α-glucosidases from Bifidobacterium adolescentis DSM20083. Appl Microbiol Biotechnol 61, 55–60 (2003). https://doi.org/10.1007/s00253-002-1179-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-002-1179-1

Keywords

Search

Navigation