Abstract
Mycobacterium sp. VKM Ac-1815D and its derivatives with altered resistance to antibacterial agents were able to produce androst-4-ene-3,17-dione (AD) as a major product from sitosterol. In this study, those strains were subjected to subsequent mutagenization by chemical agents and UV irradiation in combination with sitosterol selection pressure. The mutant Mycobacterium sp. 2-4 M was selected, being capable of producing 9α-hydroxyandrost-4-ene-3,17-dione (9-OH-AD) as a major product from sitosterol, with a 50% molar yield. Along with 9-OH-AD, both AD and 9α-hydroxylated metabolites with a partially degraded side-chain were formed from sitosterol by the mutant strain. The strain was unable to degrade 9-OH-AD, but degraded androsta-1,4-diene-3,17-dione (ADD), thus indicating a deficiency in steroid 1(2)-dehydrogenase and the presence of 9α-hydroxylase activity.
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Donova, M.V., Gulevskaya, S.A., Dovbnya, D.V. et al. Mycobacterium sp. mutant strain producing 9α-hydroxyandrostenedione from sitosterol. Appl Microbiol Biotechnol 67, 671–678 (2005). https://doi.org/10.1007/s00253-004-1808-y
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DOI: https://doi.org/10.1007/s00253-004-1808-y