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Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport

  • Applied Genetics and Molecular Biotechnology
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Abstract

Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport.

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Acknowledgements

We thank Marieke Escher for technical assistance. This work was supported by grants from the European Union (EUROFUNG), contract numbers BIO4CT96-0535 and QTRK3-199-00729 to J.V.

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Author notes

  1. Peter J. I. van de Vondervoort

    Present address: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands

  2. Marco J. L. de Groot

    Present address: Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA, Utrecht, The Netherlands

  3. George J. G. Ruijter

    Present address: Department Clinical Genetics, Eramus Medical Centre, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

  4. Jaap Visser

    Present address: FGT Consultancy, P.O. Box 396, 6700 AJ, Wageningen, The Netherlands

Authors and Affiliations

  1. Section Molecular Genetics of Industrial Microorganisms, Wageningen University, Dreijenlaan 2, 6703 HA, Wageningen, The Netherlands

    Peter J. I. van de Vondervoort, Marco J. L. de Groot, George J. G. Ruijter & Jaap Visser

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  1. Peter J. I. van de Vondervoort
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  2. Marco J. L. de Groot
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  3. George J. G. Ruijter
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Corresponding author

Correspondence to Peter J. I. van de Vondervoort.

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The first two authors have contributed equally to this work.

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van de Vondervoort, P.J.I., de Groot, M.J.L., Ruijter, G.J.G. et al. Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport. Appl Microbiol Biotechnol 73, 881–886 (2006). https://doi.org/10.1007/s00253-006-0527-y

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  • DOI: https://doi.org/10.1007/s00253-006-0527-y

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