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Differential regulation of manganese peroxidases and characterization of two variable MnP encoding genes in the white-rot fungus Physisporinus rivulosus

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Abstract

Manganese peroxidase (MnP) production in the white-rot basidiomycete Physisporinus rivulosus T241i was studied. Separate MnP isoforms were produced in carbon-limited liquid media supplemented with Mn2+, veratryl alcohol, or sawdust. The isoforms had different pH ranges for the oxidation of Mn2+ and 2,6-dimethoxyphenol. Although lignin degradation by white-rot fungi is often triggered by nitrogen depletion, MnPs of P. rivulosus were efficiently produced also in the presence of high-nutrient nitrogen, especially in cultures supplemented with veratryl alcohol. Two MnP encoding genes, mnpA and mnpB, were identified, and their corresponding cDNAs were characterized. Structurally, the genes showed marked dissimilarity, and the expression of the two genes implicated quantitative variation and differential regulation in response to manganese, veratryl alcohol, or sawdust. The variability in regulation and properties of the isoforms may widen the operating range for efficient lignin degradation by P. rivulosus.

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Abbreviations

aa:

amino acid

cDNA:

complementary DNA

gDNA:

genomic DNA

HC:

high-nutrient carbon

HN:

high-nutrient nitrogen

LC:

low-nutrient carbon

LiP:

lignin peroxidase (EC 1.11.1.14)

LN:

low-nutrient nitrogen

MnP:

manganese peroxidase (EC 1.11.1.13)

RACE:

rapid amplification of cDNA ends

RT:

reverse transcription

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Acknowledgements

This work was supported by the Academy of Finland (Center of Excellence “Microbial Resources Research Unit” grant no. 53305 and research grant no. 205027 to K.H.). Terhi K. Hakala was supported by Viikki Graduate School of Biosciences, University of Helsinki.

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Author notes

  1. Terhi K. Hakala

    Present address: KCL Science and Consulting, Espoo, Finland

Authors and Affiliations

  1. Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, Biocenter 1, 00014, Helsinki, Finland

    Terhi K. Hakala, Kristiina Hildén, Pekka Maijala & Annele Hatakka

  2. Program of Developmental and Reproductive Biology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland

    Cia Olsson

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  1. Terhi K. Hakala
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  2. Kristiina Hildén
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Correspondence to Kristiina Hildén.

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Hakala, T.K., Hildén, K., Maijala, P. et al. Differential regulation of manganese peroxidases and characterization of two variable MnP encoding genes in the white-rot fungus Physisporinus rivulosus . Appl Microbiol Biotechnol 73, 839–849 (2006). https://doi.org/10.1007/s00253-006-0541-0

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