Skip to main content

Advertisement

SpringerLink
Log in

Comparison of endoglucanase-1 (EG1) induction in the edible straw mushroom Volvariella volvacea by lactose and/or cellobiose with or without added sorbose

  • Applied Microbial and Cell Physiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

We have compared the induction of an endoglucanase (EG1) by α-lactose and/or cellobiose, with or without added l-sorbose, in submerged cultures of Volvariella volvacea, to better understand the mechanism whereby cellulase formation is triggered by these soluble disaccharides. EG1 levels induced by α-lactose and cellobiose were 28.6% and 6.7%, respectively of the highest levels recorded with crystalline cellulose. Sorbose did not induce EG1 and strongly repressed enzyme levels when added to α-lactose but not cellobiose-containing cultures. EG1 levels in cultures containing all three saccharides were similar to those recorded with sorbose and cellobiose although enzyme induction was delayed by 12 h. When V. volvacea was pre-grown for 24 h in medium containing sorbose as the sole carbon source, followed by addition of α-lactose or cellobiose or a mixture of the two, EG1 levels recorded in the α-lactose-supplemented cultures were again markedly lower compared with cultures containing only α-lactose. Maximal enzyme levels in cultures with added cellobiose or cellobiose and α-lactose were not affected although appearance of EG1 in culture supernatants was again delayed by 12 h. Semi-quantitative RT-PCR revealed that higher, more prolonged, levels of eg1 transcription occurred in V. volvacea cultures induced with α-lactose compared with cellobiose- or α-lactose + cellobiose-induced cultures. However, eg1 transcription levels in cultures induced with cellobiose or with cellobiose + lactose, and the corresponding cultures with added sorbose, were not markedly different.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Aro N, Pakula T, Penttilä M (2005) Transcriptional regulation of plant cell wall degradation by filamentous fungi. FEMS Microbiol Rev 29:719–739

    Article  CAS  Google Scholar 

  • Birch PR (1998) Targeted differential display of abundantly expressed sequences from the basidiomycete Phanerochaete chrysosporium which contain regions coding for fungal cellulose-binding domains. Curr Genet 33:70–76

    Article  CAS  Google Scholar 

  • Bisaria VS, Mishra S (1989) Regulatory aspects of cellulase biosynthesis and secretion. CRC Crit Rev Biotechnol 9:61–103

    Article  CAS  Google Scholar 

  • Broda P, Birch PR, Brooks PR, Sims PF (1995) PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families. Appl Environ Microbiol 61:2358–2364

    CAS  Google Scholar 

  • Cai YJ, Buswell JA, Chang ST (1998) β-Glucosidase components of the cellulolytic system of the edible straw mushroom, Volvariella volvacea. Enzyme Microb Technol 22:122–129

    Article  CAS  Google Scholar 

  • Chang ST (1999) World production of cultivated edible and medicinal mushrooms in 1997 with emphasis on Lentinus edodes (Berk.) Sing. in China. Int J Med Mushr 1:291–300

    Google Scholar 

  • Chow CM, Yague E, Raguz S, Wood DA, Thurston CF (1994) The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source. Appl Environ Microbiol 60:2779–2785

    CAS  Google Scholar 

  • De Groot PWJ, Visser J, Van Griensven LJD, Schaap PJ (1998) Biochemical and molecular aspects of growth and fruiting of the edible mushroom Agaricus bisporus. Mycol Res 102:1297–1308

    Article  Google Scholar 

  • Ding SJ, Buswell JA, Ge W (2001) Endoglucanase I from the edible straw mushroom, Volvariella volvacea: purification, characterization, cloning and expression. Eur J Biochem 268:5687–5695

    Article  CAS  Google Scholar 

  • Ding SJ, Buswell JA, Ge W (2002) Secretion, purification and characterization of a recombinant Volvariella volvacea endoglucanase expressed in the yeast Pichia pastoris. Enzyme Microb Technol 31:621–626

    Article  CAS  Google Scholar 

  • Ding SJ, Ge W, Buswell JA (2006) Cloning of multiple cellulase cDNAs from Volvariella volvacea and their differential expression during substrate colonization and fruiting. FEMS Microbiol Lett 263:207–213

    Article  CAS  Google Scholar 

  • Ding SJ, Buswell JA, Ge W (2007) Molecular cloning and transcriptional expression analysis of an intracellular β-glucosidase, a family 3 glycosyl hydrolase, from the edible straw mushroom, Volvariella volvacea. FEMS Microbiol Lett 267:221–229

    Article  CAS  Google Scholar 

  • Fang X, Yano S, Inoue H, Sawayama S (2008) Lactose enhances cellulase production by the filamentous fungus Acremonium cellulolyticus. J Biosci Bioeng 106:115–120

    Article  CAS  Google Scholar 

  • Fekete E, Karaffa L, Kubicek CP, Szentirmai A, Seiboth B (2007) Induction of extracellular β-galactosidase (Bga1) formation by d-galactose in Hypocrea jecorina is mediated by galactitol. Microbiology 153:507–512

    Article  CAS  Google Scholar 

  • Gielkens MM, Dekkers E, Visser J, de Graaff LH (1999) Two cellobiohydrolase-encoding genes from Aspergillus niger require d-xylose and the xylanolytic transcriptional activator XlnR for their expression. Appl Environ Microbiol 65:4340–4345

    CAS  Google Scholar 

  • Hartl L, Kubicek CP, Seiboth B (2007) Induction of the gal pathway and cellulase genes involves no transcriptional inducer function of the galactokinase in Hypocrea jecorina. J Biol Chem 282:18654–18659

    Article  CAS  Google Scholar 

  • Ilmen M, Saloheimo A, Onnela ML, Penttilä ME (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63:1298–1306

    CAS  Google Scholar 

  • Karaffa L, Fekete E, Gamauf C, Szentirmai A, Kubicek CP, Seiboth B (2006) D-Galactose induces cellulase gene expression in Hypocrea jecorina at low growth rates. Microbiology 152:1507–1514

    Article  CAS  Google Scholar 

  • Kubicek CP, Messner R, Gruber F, Mach RL, Kubicek-Pranz EM (1993) The Trichoderma cellulase regulatory puzzle: from the interior life of a secretory fungus. Enzyme Microb Technol 15:90–99

    Article  CAS  Google Scholar 

  • Kües Y, Lui Y (2000) Fruiting body production in Basidiomycetes. Appl Microbiol Biotechnol 54:141–152

    Article  Google Scholar 

  • Kurasawa T, Yachi M, Suto M, Kamagata Y, Takao S, Tomita F (1992) Induction of cellulase by gentiobiose and its sulfur-containing analog in Penicillium purpurogenum. Appl Environ Microbiol 58:106–110

    CAS  Google Scholar 

  • Mach RL, Zeilinger S (2003) Regulation of gene expression in industrial fungi: Trichoderma. Appl Microbiol Biotechnol 60:515–522

    CAS  Google Scholar 

  • Morikawa Y, Ohashi T, Mantani O, Okada H (1995) Cellulase induction by lactose in Trichoderma reesei PC-3-7. Appl Microbiol Biotechnol 44:106–111

    Article  CAS  Google Scholar 

  • Nogawa M, Goto M, Okada H, Morikawa Y (2001) L-Sorbose induces cellulase gene transcription in the cellulolytic fungus Trichoderma reesei. Curr Genet 38:329–334

    Article  CAS  Google Scholar 

  • Penttilä ME (1998) Heterologous protein production in Trichoderma. In: Harman GE, Kubicek CP (eds) Trichoderma and Gliocladium: enzymes, biological control and commercial applications. Taylor and Francis, London, pp 365–382

    Google Scholar 

  • Sachslehner A, Nidetzky B, Kulbe KD, Haltrich D (1998) Induction of mannanase, xylanase, and endoglucanase activities in Sclerotium rolfsii. Appl Environ Microbiol 64:594–600

    CAS  Google Scholar 

  • Sehnem NT, de Bittencourt LR, Camassola M, Dillon AJ (2006) Cellulase production by Penicillium echinulatum on lactose. Appl Microbiol Biotechnol 72:163–167

    Article  CAS  Google Scholar 

  • Seiboth B, Hofmann G, Kubicek CP (2002) Lactose metabolism and cellulase production in Hypocrea jecorina: the gal7 gene, encoding galactose-1-phosphate uridylyltransferase, is essential for growth on galactose but not for cellulase induction. Mol Genet Genomics 267:124–132

    Article  CAS  Google Scholar 

  • Seiboth B, Hartl L, Pail M, Fekete E, Karaffa L, Kubicek CP (2004) The galactokinase of Hypocrea jecorina is essential for cellulase induction by lactose but dispensable for growth on d-galactose. Mol Microbiol 51:1015–1025

    Article  CAS  Google Scholar 

  • Seiboth B, Hartl L, Salovuori N, Lanthaler K, Robson GD, Vehmaanpera J, Penttila ME, Kubicek CP (2005) Role of the bga1-encoded extracellular β-galactosidase of Hypocrea jecorina in cellulase induction by lactose. Appl Environ Microbiol 71:851–857

    Article  CAS  Google Scholar 

  • Stills HF, Bailey MQ (1991) The use of Freund’s complete adjuvant. Lab Anim 20:25–31

    Google Scholar 

  • Stricker AR, Steiger MG, Mach RL (2007) Xyr1 receives the lactose induction signal and regulates lactose metabolism in Hypocrea jecorina. FEBS Lett 581:3915–3920

    Article  CAS  Google Scholar 

  • Suto M, Tomita F (2001) Induction and catabolite repression mechanisms of cellulase in fungi. J Biosci Bioeng 92:305–311

    Article  CAS  Google Scholar 

  • Teeri TT, Penttilä M, Keränen S, Nevalainen H, Knowles JK (1992) Structure, function, and genetics of cellulases. Biotechnology 21:417–445

    CAS  Google Scholar 

  • Wang J, Guo L, Zhang K, Wu Q, Lin J (2008) Highly efficient Agrobacterium-mediated transformation of Volvariella volvacea. Bioresour Technol 99:8524–8527

    Article  CAS  Google Scholar 

  • Yague E, Mehak-Zunic M, Morgan L, Wood DA, Thurston CF (1997) Expression of CEL2 and CEL4, two proteins from Agaricus bisporus with similarity to fungal cellobiohydrolase I and β-mannanase, respectively, is regulated by the carbon source. Microbiology 143:239–244

    Article  CAS  Google Scholar 

  • Zhang YH, Lynd LR (2005) Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum. J Bacteriol 187:99–106

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was supported by a research grant (No. 30400301) from the National Natural Science Foundation of China.

Author information

Authors and Affiliations

  1. Department of Biological Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, People’s Republic of China

    Liang Zhang, Yun Pei & Shaojun Ding

  2. Institute of Agri-food Standards and Technology Testing, Shanghai, China

    Zengtao Xing

  3. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China

    John A. Buswell

Authors
  1. Liang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yun Pei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zengtao Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shaojun Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. John A. Buswell
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shaojun Ding.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, L., Pei, Y., Xing, Z. et al. Comparison of endoglucanase-1 (EG1) induction in the edible straw mushroom Volvariella volvacea by lactose and/or cellobiose with or without added sorbose. Appl Microbiol Biotechnol 89, 1939–1946 (2011). https://doi.org/10.1007/s00253-010-2995-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-010-2995-3

Keywords

Search

Navigation